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Protein

Proline--tRNA ligase

Gene

proS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine. The misacylated Cys-tRNA(Pro) is not edited by ProRS.

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

Kineticsi

  1. KM=0.28 mM for proline1 Publication
  2. KM=0.17 mM for cysteine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRPAL258594:G1GT2-3009-MONOMER
    BRENDAi6.1.1.15 5412
    SABIO-RKiQ6N5P6

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase (EC:6.1.1.15)
    Alternative name(s):
    Prolyl-tRNA synthetase
    Short name:
    ProRS
    Gene namesi
    Name:proS
    Ordered Locus Names:RPA2928
    OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
    Taxonomic identifieri258594 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
    Proteomesi
    • UP000001426 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002482271 – 438Proline--tRNA ligaseAdd BLAST438

    Proteomic databases

    PRIDEiQ6N5P6

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    DIPiDIP-29058N
    STRINGi258594.RPA2928

    Structurei

    Secondary structure

    1438
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 5Combined sources3
    Helixi21 – 28Combined sources8
    Beta strandi32 – 36Combined sources5
    Beta strandi39 – 42Combined sources4
    Helixi44 – 63Combined sources20
    Beta strandi73 – 76Combined sources4
    Helixi77 – 82Combined sources6
    Helixi85 – 88Combined sources4
    Helixi90 – 92Combined sources3
    Beta strandi93 – 97Combined sources5
    Beta strandi103 – 106Combined sources4
    Helixi111 – 121Combined sources11
    Helixi125 – 127Combined sources3
    Beta strandi129 – 139Combined sources11
    Helixi148 – 150Combined sources3
    Beta strandi153 – 166Combined sources14
    Helixi167 – 186Combined sources20
    Turni187 – 189Combined sources3
    Beta strandi192 – 196Combined sources5
    Helixi200 – 202Combined sources3
    Beta strandi207 – 213Combined sources7
    Beta strandi218 – 224Combined sources7
    Helixi225 – 229Combined sources5
    Beta strandi239 – 241Combined sources3
    Helixi244 – 252Combined sources9
    Beta strandi255 – 257Combined sources3
    Turni258 – 260Combined sources3
    Helixi265 – 269Combined sources5
    Turni272 – 274Combined sources3
    Beta strandi275 – 290Combined sources16
    Helixi292 – 296Combined sources5
    Beta strandi300 – 302Combined sources3
    Beta strandi308 – 310Combined sources3
    Beta strandi312 – 319Combined sources8
    Helixi320 – 330Combined sources11
    Beta strandi331 – 333Combined sources3
    Turni341 – 343Combined sources3
    Beta strandi347 – 354Combined sources8
    Helixi358 – 373Combined sources16
    Beta strandi378 – 381Combined sources4
    Helixi387 – 397Combined sources11
    Beta strandi400 – 405Combined sources6
    Helixi407 – 410Combined sources4
    Turni411 – 413Combined sources3
    Beta strandi414 – 419Combined sources6
    Turni420 – 422Combined sources3
    Beta strandi425 – 429Combined sources5
    Turni430 – 434Combined sources5
    Turni435 – 437Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2I4LX-ray2.00A/B/C1-438[»]
    2I4MX-ray2.80A/B/C1-438[»]
    2I4NX-ray2.85A/B/C1-438[»]
    2I4OX-ray2.40A/B/C1-438[»]
    ProteinModelPortaliQ6N5P6
    SMRiQ6N5P6
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6N5P6

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C90 Bacteria
    COG0442 LUCA
    HOGENOMiHOG000076894
    KOiK01881
    OMAiIQPAELW
    OrthoDBiPOG091H0290
    PhylomeDBiQ6N5P6

    Family and domain databases

    CDDicd00779 ProRS_core_prok, 1 hit
    Gene3Di3.40.50.800, 1 hit
    HAMAPiMF_01570 Pro_tRNA_synth_type2, 1 hit
    InterProiView protein in InterPro
    IPR002314 aa-tRNA-synt_IIb
    IPR006195 aa-tRNA-synth_II
    IPR004154 Anticodon-bd
    IPR036621 Anticodon-bd_dom_sf
    IPR002316 Pro-tRNA-ligase_IIa
    IPR004500 Pro-tRNA-synth_IIa_bac-type
    IPR023716 Prolyl-tRNA_ligase_IIa_type2
    IPR033730 ProRS_core_prok
    PfamiView protein in Pfam
    PF03129 HGTP_anticodon, 1 hit
    PF00587 tRNA-synt_2b, 1 hit
    PRINTSiPR01046 TRNASYNTHPRO
    TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
    PROSITEiView protein in PROSITE
    PS50862 AA_TRNA_LIGASE_II, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q6N5P6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLSRFFLPI LKENPKEAEI VSHRLMLRAG MLRQEAAGIY AWLPLGHRVL
    60 70 80 90 100
    KKIEQIVREE QNRAGAIELL MPTLQLADLW RESGRYDAYG PEMLRIADRH
    110 120 130 140 150
    KRELLYGPTN EEMITEIFRA YIKSYKSLPL NLYHIQWKFR DEQRPRFGVM
    160 170 180 190 200
    RGREFLMKDA YSFDVDEAGA RKSYNKMFVA YLRTFARMGL KAIPMRAETG
    210 220 230 240 250
    PIGGDLSHEF IVLAETGESG VYIDRDVLNL PVPDENVDYD GDLTPIIKQW
    260 270 280 290 300
    TSVYAATEDV HEPARYESEV PEANRLNTRG IEVGQIFYFG TKYSDSMKAN
    310 320 330 340 350
    VTGPDGTDAP IHGGSYGVGV SRLLGAIIEA CHDDNGIIWP EAVAPFRVTI
    360 370 380 390 400
    LNLKQGDAAT DAACDQLYRE LSAKGVDVLY DDTDQRAGAK FATADLIGIP
    410 420 430
    WQIHVGPRGL AEGKVELKRR SDGARENLAL ADVVARLT
    Length:438
    Mass (Da):49,309
    Last modified:July 5, 2004 - v1
    Checksum:i36D07FB76904F42A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX572602 Genomic DNA Translation: CAE28369.1
    RefSeqiWP_011158477.1, NC_005296.1

    Genome annotation databases

    EnsemblBacteriaiCAE28369; CAE28369; RPA2928
    KEGGirpa:RPA2928

    Similar proteinsi

    Entry informationi

    Entry nameiSYP_RHOPA
    AccessioniPrimary (citable) accession number: Q6N5P6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: July 5, 2004
    Last modified: May 23, 2018
    This is version 100 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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