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Protein

Proline--tRNA ligase

Gene

proS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine. The misacylated Cys-tRNA(Pro) is not edited by ProRS.

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

Kineticsi

  1. KM=0.28 mM for proline1 Publication
  2. KM=0.17 mM for cysteine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.1.1.15. 5412.
    SABIO-RKQ6N5P6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase (EC:6.1.1.15)
    Alternative name(s):
    Prolyl-tRNA synthetase
    Short name:
    ProRS
    Gene namesi
    Name:proS
    Ordered Locus Names:RPA2928
    OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
    Taxonomic identifieri258594 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
    Proteomesi
    • UP000001426 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002482271 – 438Proline--tRNA ligaseAdd BLAST438

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    DIPiDIP-29058N.
    STRINGi258594.RPA2928.

    Structurei

    Secondary structure

    1438
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 5Combined sources3
    Helixi21 – 28Combined sources8
    Beta strandi32 – 36Combined sources5
    Beta strandi39 – 42Combined sources4
    Helixi44 – 63Combined sources20
    Beta strandi73 – 76Combined sources4
    Helixi77 – 82Combined sources6
    Helixi85 – 88Combined sources4
    Helixi90 – 92Combined sources3
    Beta strandi93 – 97Combined sources5
    Beta strandi103 – 106Combined sources4
    Helixi111 – 121Combined sources11
    Helixi125 – 127Combined sources3
    Beta strandi129 – 139Combined sources11
    Helixi148 – 150Combined sources3
    Beta strandi153 – 166Combined sources14
    Helixi167 – 186Combined sources20
    Turni187 – 189Combined sources3
    Beta strandi192 – 196Combined sources5
    Helixi200 – 202Combined sources3
    Beta strandi207 – 213Combined sources7
    Beta strandi218 – 224Combined sources7
    Helixi225 – 229Combined sources5
    Beta strandi239 – 241Combined sources3
    Helixi244 – 252Combined sources9
    Beta strandi255 – 257Combined sources3
    Turni258 – 260Combined sources3
    Helixi265 – 269Combined sources5
    Turni272 – 274Combined sources3
    Beta strandi275 – 290Combined sources16
    Helixi292 – 296Combined sources5
    Beta strandi300 – 302Combined sources3
    Beta strandi308 – 310Combined sources3
    Beta strandi312 – 319Combined sources8
    Helixi320 – 330Combined sources11
    Beta strandi331 – 333Combined sources3
    Turni341 – 343Combined sources3
    Beta strandi347 – 354Combined sources8
    Helixi358 – 373Combined sources16
    Beta strandi378 – 381Combined sources4
    Helixi387 – 397Combined sources11
    Beta strandi400 – 405Combined sources6
    Helixi407 – 410Combined sources4
    Turni411 – 413Combined sources3
    Beta strandi414 – 419Combined sources6
    Turni420 – 422Combined sources3
    Beta strandi425 – 429Combined sources5
    Turni430 – 434Combined sources5
    Turni435 – 437Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2I4LX-ray2.00A/B/C1-438[»]
    2I4MX-ray2.80A/B/C1-438[»]
    2I4NX-ray2.85A/B/C1-438[»]
    2I4OX-ray2.40A/B/C1-438[»]
    ProteinModelPortaliQ6N5P6.
    SMRiQ6N5P6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6N5P6.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C90. Bacteria.
    COG0442. LUCA.
    HOGENOMiHOG000076894.
    KOiK01881.
    OMAiIQPAELW.
    OrthoDBiPOG091H0290.
    PhylomeDBiQ6N5P6.

    Family and domain databases

    CDDicd00779. ProRS_core_prok. 1 hit.
    Gene3Di3.40.50.800. 1 hit.
    HAMAPiMF_01570. Pro_tRNA_synth_type2. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023716. Prolyl-tRNA_ligase_IIa_type2.
    IPR033730. ProRS_core_prok.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6N5P6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLSRFFLPI LKENPKEAEI VSHRLMLRAG MLRQEAAGIY AWLPLGHRVL
    60 70 80 90 100
    KKIEQIVREE QNRAGAIELL MPTLQLADLW RESGRYDAYG PEMLRIADRH
    110 120 130 140 150
    KRELLYGPTN EEMITEIFRA YIKSYKSLPL NLYHIQWKFR DEQRPRFGVM
    160 170 180 190 200
    RGREFLMKDA YSFDVDEAGA RKSYNKMFVA YLRTFARMGL KAIPMRAETG
    210 220 230 240 250
    PIGGDLSHEF IVLAETGESG VYIDRDVLNL PVPDENVDYD GDLTPIIKQW
    260 270 280 290 300
    TSVYAATEDV HEPARYESEV PEANRLNTRG IEVGQIFYFG TKYSDSMKAN
    310 320 330 340 350
    VTGPDGTDAP IHGGSYGVGV SRLLGAIIEA CHDDNGIIWP EAVAPFRVTI
    360 370 380 390 400
    LNLKQGDAAT DAACDQLYRE LSAKGVDVLY DDTDQRAGAK FATADLIGIP
    410 420 430
    WQIHVGPRGL AEGKVELKRR SDGARENLAL ADVVARLT
    Length:438
    Mass (Da):49,309
    Last modified:July 5, 2004 - v1
    Checksum:i36D07FB76904F42A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX572602 Genomic DNA. Translation: CAE28369.1.
    RefSeqiWP_011158477.1. NC_005296.1.

    Genome annotation databases

    EnsemblBacteriaiCAE28369; CAE28369; RPA2928.
    KEGGirpa:RPA2928.
    PATRICi23290299. VBIRhoPal84835_3076.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX572602 Genomic DNA. Translation: CAE28369.1.
    RefSeqiWP_011158477.1. NC_005296.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2I4LX-ray2.00A/B/C1-438[»]
    2I4MX-ray2.80A/B/C1-438[»]
    2I4NX-ray2.85A/B/C1-438[»]
    2I4OX-ray2.40A/B/C1-438[»]
    ProteinModelPortaliQ6N5P6.
    SMRiQ6N5P6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-29058N.
    STRINGi258594.RPA2928.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAE28369; CAE28369; RPA2928.
    KEGGirpa:RPA2928.
    PATRICi23290299. VBIRhoPal84835_3076.

    Phylogenomic databases

    eggNOGiENOG4105C90. Bacteria.
    COG0442. LUCA.
    HOGENOMiHOG000076894.
    KOiK01881.
    OMAiIQPAELW.
    OrthoDBiPOG091H0290.
    PhylomeDBiQ6N5P6.

    Enzyme and pathway databases

    BRENDAi6.1.1.15. 5412.
    SABIO-RKQ6N5P6.

    Miscellaneous databases

    EvolutionaryTraceiQ6N5P6.

    Family and domain databases

    CDDicd00779. ProRS_core_prok. 1 hit.
    Gene3Di3.40.50.800. 1 hit.
    HAMAPiMF_01570. Pro_tRNA_synth_type2. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023716. Prolyl-tRNA_ligase_IIa_type2.
    IPR033730. ProRS_core_prok.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSYP_RHOPA
    AccessioniPrimary (citable) accession number: Q6N5P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: July 5, 2004
    Last modified: November 2, 2016
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.