Q6N5K3 (BIOD_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	RPA2971

Organism

Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]

Taxonomic identifier

258594 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302543
Regions
	Nucleotide binding	100 – 103	4	ATP By similarity
	Nucleotide binding	184 – 186	3	ATP By similarity
Sites
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Binding site	37	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6N5K3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C7AA968793795236	FASTA	212	22,562
	10 20 30 40 50 60 MSARIVVTGT DTGIGKTVFA AALAGALDAT YWKPVQSGLE DETDSGAVQR LSGLAADRIL 70 80 90 100 110 120 PERYRLQTPA SPHLAAEIDG VDIDVAALEL PSVSRPLVVE GAGGLMVPLT RETTYIDVFA 130 140 150 160 170 180 RWAAPLVLCA RTSLGTINHT LLSIEAIRAR DIPLLGVAFL GDENLDSEQI IVELGHTRRL 190 200 210 GRLPRLERLD AAALRAGFAA AFEPRDFLGD AP

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572602 Genomic DNA. Translation: CAE28412.1.
RefSeq	NP_948312.1. NC_005296.1.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ProteinModelPortal	Q6N5K3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2691795.
GenomeReviews	Gene locus RPA2971 in contig BX571963_GR.
KEGG	rpa:RPA2971.
NMPDR	fig|258594.1.peg.2955.
PATRIC	23290399. VBIRhoPal84835_3123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA2971-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_RHOPA

Accession

Primary (citable) accession number: Q6N5K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program