Q6N5D6 (SYD_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	RPA3043

Organism

Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]

Taxonomic identifier

258594 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110931

Sequences

	Sequence		Length	Mass (Da)
	Q6N5D6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 39B34E2884F56ACE	FASTA	591	66,654
	10 20 30 40 50 60 MHRYRTHTCG ALRDSHIDQT VRLSGWCHRI RDHGGVLFID LRDHYGLTQC VADPDSPAFK 70 80 90 100 110 120 DAEKLRAEWV VRIDGKVRRR PEGTDNPDLP TGAVEVFVTE IEVLGPAGEL PLPVFGEQEY 130 140 150 160 170 180 PEDVRLRYRF LDLRREKLHQ NIMTRGAIVD SMRRRMKEQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFVTQDDIF AAMEPVITGV FEEFAKGKRV TKGWPRIAFA DSMRKYGTDK PDLRNPIEMQ 310 320 330 340 350 360 DVSEHFRGSG FKVFARMLEE QRNQVWAIPG PGGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGGEGAGPLA NNIGPERTEA IRAALGLKAG DAAFFVAGDP SKFVKFAGLA RTKVGEELNL 430 440 450 460 470 480 IDKDQFALAW VVDFPMYEYN EDDKKVDFSH NPFSMPQGGM EALTSQDPLT IKAFQYDITC 490 500 510 520 530 540 NGYEIASGGI RNHRPEAMVK AFEIAGYGEQ EVVDRFGGMY RAFQYGAPPH GGMAAGVDRI 550 560 570 580 590 VMLLCGTTNL REISLFPMNQ RAEDLLMGAP SEVSPKQLRE LHIRLNLPDT K

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572602 Genomic DNA. Translation: CAE28484.1.
RefSeq	NP_948382.1. NC_005296.1.
3D structure databases
ProteinModelPortal	Q6N5D6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2691254.
GenomeReviews	Gene locus RPA3043 in contig BX571963_GR.
KEGG	rpa:RPA3043.
NMPDR	fig|258594.1.peg.3025.
PATRIC	23290547. VBIRhoPal84835_3197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA3043-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_RHOPA

Accession

Primary (citable) accession number: Q6N5D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program