Reviewed, UniProtKB/Swiss-Prot Q6N4T2 (RS12_RHOPA)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S12
Alternative name(s):
    RRP-S12

Gene names

Name:	rpsL
Ordered Locus Names:	RPA3255

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	With S4 and S5 plays an important role in translational accuracy By similarity. Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit By similarity.
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex By similarity.
Post-translational modification	Both N-terminus methionine truncation and retention have been observed for this protein. HAMAP MF_00403
Sequence similarities	Belongs to the ribosomal protein S12P family.
Caution	Both N-terminus methionine truncation and retention have been observed for this protein by 2 different experiments.
Mass spectrometry	Molecular mass is 13875.2 Da from positions 1 - 123. Determined by ESI. This is the mass for the protein without N-terminus methionine removal. Ref.2

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	30S ribosomal protein S12 HAMAP MF_00403		PRO_0000146297
Amino acid modifications
	Modified residue	89	1	3-methylthioaspartic acid HAMAP MF_00403

Sequences

	Sequence		Length	Mass (Da)
	Q6N4T2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 26E3A2843874F549	FASTA	123	13,875
	10 20 30 40 50 60 MPTINQLIAN PRVVQKSRKK VPALQQSPQK RGVCTRVYTT TPKKPNSALR KVAKVRLTNG 70 80 90 100 110 120 FEVIGYIPGE GHNLQEHSVV MIRGGRVKDL PGVRYHILRG VLDTQGVKNR KQRRSKYGAK RPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.
[2]	"Characterization of the 70S ribosome from Rhodopseudomonas palustris using an integrated 'top-down' and 'bottom-up' mass spectrometric approach." Strader M.B., VerBerkmoes N.C., Tabb D.L., Connelly H.M., Barton J.W., Bruce B.D., Pelletier D.A., Davison B.H., Hettich R.L., Larimer F.W., Hurst G.B. J. Proteome Res. 3:965-978(2004) [PubMed: 15473684] [Abstract] Cited for: BETA-METHYLTHIOLATION AT ASP-89, MASS SPECTROMETRY. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572603 Genomic DNA. Translation: CAE28696.1.
RefSeq	NP_948594.1.
3D structure databases
SMR	Q6N4T2. Positions 3-123.
ModBase	Search...
Genome annotation databases
GeneID	2691958.
GenomeReviews	Gene locus RPA3255 in contig BX571963_GR.
KEGG	rpa:RPA3255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6N4T2.
OMA	SPALKSC.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA3255-MON.
Family and domain databases
HAMAP	MF_00403. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR006032. Ribosomal_S12/S23. IPR005679. Ribosomal_S12_bac-type. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR11652:SF1. Ribosom_S12_bac. 1 hit. PTHR11652. Ribosomal_S12_23. 1 hit.
Pfam	PF00164. Ribosomal_S12. 1 hit. [Graphical view]
PIRSF	PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTS	PR01034. RIBOSOMALS12.
TIGRFAMs	TIGR00981. rpsL_bact. 1 hit.
PROSITE	PS00055. RIBOSOMAL_S12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS12_RHOPA

Accession

Primary (citable) accession number: Q6N4T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)