Reviewed, UniProtKB/Swiss-Prot Q6N3G0 (ISPH1_RHOPA)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1
    EC=1.17.1.2

Gene names

Name:	ispH1
Synonyms:	lytB1
Ordered Locus Names:	RPA3734

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 HAMAP MF_00191		PRO_0000128865

Sequences

	Sequence		Length	Mass (Da)
	Q6N3G0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8F33416B324564EA	FASTA	306	32,905
	10 20 30 40 50 60 MFLAQPRGFC AGVVRAIEIV ERALEKYGPP VYVRHEIVHN KYVVESLKAK GAIFVEELSE 70 80 90 100 110 120 VPPKAVTVFS AHGVARSIEE EAAARDLPVL NATCPLVTKV HNQGKRYTSK GRTLILIGHA 130 140 150 160 170 180 GHPEVEGTMG QVPGPVLLVQ NLDDVAALTL PPETPVAYIT QTTLSVDDTK DIIVALSKKF 190 200 210 220 230 240 HDIEGPDTRD ICYATQNRQS AVRQMSKLVD LILVVGANNS SNSNRLREIG TEAGIPSYLI 250 260 270 280 290 300 ADGSELDPAW LDGKKAVGIT AGASAPEVLV DDVIEALRRI VPVTVSVLPG REENIEFVLP AELATA

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572605 Genomic DNA. Translation: CAE29175.1.
RefSeq	NP_949071.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2690489.
KEGG	rpa:RPA3734.
NMPDR	fig|258594.1.peg.3714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	REENIEF.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA3734-MONOMER.
BRENDA	1.17.1.2. 373.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH1_RHOPA

Accession

Primary (citable) accession number: Q6N3G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)