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Q6N234 (MASZ_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G

EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:RPA4216
OrganismRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]
Taxonomic identifier258594 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity. HAMAP-Rule MF_00641

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 724724Malate synthase G HAMAP-Rule MF_00641
PRO_1000056924

Regions

Region124 – 1252Acetyl-CoA binding By similarity
Region454 – 4574Glyoxylate binding By similarity

Sites

Active site3371Proton acceptor By similarity
Active site6281Proton donor By similarity
Metal binding4291Magnesium By similarity
Metal binding4571Magnesium By similarity
Binding site1171Acetyl-CoA; via carbonyl oxygen By similarity
Binding site2731Acetyl-CoA By similarity
Binding site3101Acetyl-CoA By similarity
Binding site3371Glyoxylate By similarity
Binding site4291Glyoxylate By similarity
Binding site5381Acetyl-CoA; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue6141Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6N234 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 66D835480AFC2C43

FASTA72478,627
        10         20         30         40         50         60 
MNRIDAHGLK IAPVLFDFIA KEAAPKTGIA PDVFWAGLAA IVRDLAPKTR ALLKTRDDLQ 

        70         80         90        100        110        120 
AKIDAWHLAN KGKKQDMAAY TTFLKEIGYL LPEPPTVPVE TTNIDEEIGK LCGPQLVVPL 

       130        140        150        160        170        180 
SNARYALNAA NARWGSLYDA FYGTDAIPQE ATQAKGYDKA RGDKVIAKAK AFLDQAAPLA 

       190        200        210        220        230        240 
TGSHSDVTGY SVIAGQLSAK LKSGNATGLK KPAQFAGFRG DAANPSAVLL VNNGLHIEIK 

       250        260        270        280        290        300 
IDRANTIGKD DPAGVADLVI ESAVSTILDM EDSVAAVDAD DKVLIYRNTL GLMDGTLSES 

       310        320        330        340        350        360 
FEKGGKTVTR ALNGDRTYTA PDGKEISLHG RSLLLMRNVG HHMWTDAVLD SDGQEIPEGF 

       370        380        390        400        410        420 
LDAAVSGLIA IHDLKHLGKT RNSRTGSVYI VKPKMHGPDE VALTVELFGR VETMLGLTAN 

       430        440        450        460        470        480 
TLKVGIMDEE RRTTVNLKAC IQNASKRIVF INTGFLDRTG DEIHTSMEAG PMIRKNEMKA 

       490        500        510        520        530        540 
QPWIKAYEDW NVDTGLVDGL PGHAQIGKGM WAAPDKMADM LAQKIGHPQA GATTAWVPSP 

       550        560        570        580        590        600 
TAATLHALHY HQVDVIARQQ ELAKGGPRAK LEDILTIPVS NSNWAPDDVR QEIDNNCQGI 

       610        620        630        640        650        660 
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTK EQVLDSLKRM 

       670        680        690        700        710        720 
AVIVDKQNEG DALYRPMAPD FDGVAFEAAC DLIFKGRAQP NGYTEYILHE RRREAKAAHL 


ESAR 

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References

[1]"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris."
Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S.
Nat. Biotechnol. 22:55-61(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX572606 Genomic DNA. Translation: CAE29657.1.
RefSeqNP_949552.1. NC_005296.1.

3D structure databases

ProteinModelPortalQ6N234.
SMRQ6N234. Positions 9-721.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING258594.RPA4216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE29657; CAE29657; RPA4216.
GeneID2692500.
KEGGrpa:RPA4216.
PATRIC23293025. VBIRhoPal84835_4426.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000220740.
KOK01638.
OMASQFIENE.
OrthoDBEOG6HJ286.
PhylomeDBQ6N234.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_RHOPA
AccessionPrimary (citable) accession number: Q6N234
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways