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Protein

Malate synthase G

Gene

glcB

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei273Acetyl-CoAUniRule annotation1
Binding sitei310Acetyl-CoAUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei628Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciRPAL258594:G1GT2-4336-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:RPA4216
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001426 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569241 – 724Malate synthase GAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi258594.RPA4216

Structurei

3D structure databases

ProteinModelPortaliQ6N234
SMRiQ6N234
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0
PhylomeDBiQ6N234

Family and domain databases

Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q6N234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIDAHGLK IAPVLFDFIA KEAAPKTGIA PDVFWAGLAA IVRDLAPKTR
60 70 80 90 100
ALLKTRDDLQ AKIDAWHLAN KGKKQDMAAY TTFLKEIGYL LPEPPTVPVE
110 120 130 140 150
TTNIDEEIGK LCGPQLVVPL SNARYALNAA NARWGSLYDA FYGTDAIPQE
160 170 180 190 200
ATQAKGYDKA RGDKVIAKAK AFLDQAAPLA TGSHSDVTGY SVIAGQLSAK
210 220 230 240 250
LKSGNATGLK KPAQFAGFRG DAANPSAVLL VNNGLHIEIK IDRANTIGKD
260 270 280 290 300
DPAGVADLVI ESAVSTILDM EDSVAAVDAD DKVLIYRNTL GLMDGTLSES
310 320 330 340 350
FEKGGKTVTR ALNGDRTYTA PDGKEISLHG RSLLLMRNVG HHMWTDAVLD
360 370 380 390 400
SDGQEIPEGF LDAAVSGLIA IHDLKHLGKT RNSRTGSVYI VKPKMHGPDE
410 420 430 440 450
VALTVELFGR VETMLGLTAN TLKVGIMDEE RRTTVNLKAC IQNASKRIVF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKNEMKA QPWIKAYEDW NVDTGLVDGL
510 520 530 540 550
PGHAQIGKGM WAAPDKMADM LAQKIGHPQA GATTAWVPSP TAATLHALHY
560 570 580 590 600
HQVDVIARQQ ELAKGGPRAK LEDILTIPVS NSNWAPDDVR QEIDNNCQGI
610 620 630 640 650
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTK
660 670 680 690 700
EQVLDSLKRM AVIVDKQNEG DALYRPMAPD FDGVAFEAAC DLIFKGRAQP
710 720
NGYTEYILHE RRREAKAAHL ESAR
Length:724
Mass (Da):78,627
Last modified:July 5, 2004 - v1
Checksum:i66D835480AFC2C43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572606 Genomic DNA Translation: CAE29657.1
RefSeqiWP_011159751.1, NC_005296.1

Genome annotation databases

EnsemblBacteriaiCAE29657; CAE29657; RPA4216
KEGGirpa:RPA4216

Similar proteinsi

Entry informationi

Entry nameiMASZ_RHOPA
AccessioniPrimary (citable) accession number: Q6N234
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health