Reviewed, UniProtKB/Swiss-Prot Q6N234 (MASZ_RHOPA)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	RPA4216

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Malate synthase G HAMAP MF_00641		PRO_1000056924
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	628	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6N234-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 66D835480AFC2C43	FASTA	724	78,627
	10 20 30 40 50 60 MNRIDAHGLK IAPVLFDFIA KEAAPKTGIA PDVFWAGLAA IVRDLAPKTR ALLKTRDDLQ 70 80 90 100 110 120 AKIDAWHLAN KGKKQDMAAY TTFLKEIGYL LPEPPTVPVE TTNIDEEIGK LCGPQLVVPL 130 140 150 160 170 180 SNARYALNAA NARWGSLYDA FYGTDAIPQE ATQAKGYDKA RGDKVIAKAK AFLDQAAPLA 190 200 210 220 230 240 TGSHSDVTGY SVIAGQLSAK LKSGNATGLK KPAQFAGFRG DAANPSAVLL VNNGLHIEIK 250 260 270 280 290 300 IDRANTIGKD DPAGVADLVI ESAVSTILDM EDSVAAVDAD DKVLIYRNTL GLMDGTLSES 310 320 330 340 350 360 FEKGGKTVTR ALNGDRTYTA PDGKEISLHG RSLLLMRNVG HHMWTDAVLD SDGQEIPEGF 370 380 390 400 410 420 LDAAVSGLIA IHDLKHLGKT RNSRTGSVYI VKPKMHGPDE VALTVELFGR VETMLGLTAN 430 440 450 460 470 480 TLKVGIMDEE RRTTVNLKAC IQNASKRIVF INTGFLDRTG DEIHTSMEAG PMIRKNEMKA 490 500 510 520 530 540 QPWIKAYEDW NVDTGLVDGL PGHAQIGKGM WAAPDKMADM LAQKIGHPQA GATTAWVPSP 550 560 570 580 590 600 TAATLHALHY HQVDVIARQQ ELAKGGPRAK LEDILTIPVS NSNWAPDDVR QEIDNNCQGI 610 620 630 640 650 660 LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTK EQVLDSLKRM 670 680 690 700 710 720 AVIVDKQNEG DALYRPMAPD FDGVAFEAAC DLIFKGRAQP NGYTEYILHE RRREAKAAHL ESAR

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572606 Genomic DNA. Translation: CAE29657.1.
RefSeq	NP_949552.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB P0A5J4.
SMR	Q6N234. Positions 9-721.
ModBase	Search...
Genome annotation databases
GeneID	2692500.
KEGG	rpa:RPA4216.
NMPDR	fig|258594.1.peg.4195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA4216-MONOMER.
BRENDA	2.3.3.9. 373.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHOPA

Accession

Primary (citable) accession number: Q6N234

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)