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Q6N1M8

- SYI_RHOPA

UniProt

Q6N1M8 - SYI_RHOPA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, RPA4377
Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei629 – 6291Aminoacyl-adenylate By similarity
Binding sitei673 – 6731ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RPA4377
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10051005Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098454Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi258594.RPA4377.

Structurei

3D structure databases

ProteinModelPortaliQ6N1M8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8011"HIGH" regionUniRule annotation
Add
BLAST
Motifi670 – 6745"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.
PhylomeDBiQ6N1M8.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6N1M8-1 [UniParc]FASTAAdd to Basket

« Hide

MSDKPKTDAA QSGNDYSKTL FLPQTEFPMR AGLPQREPEL LKRWEEMDLY     50
GKLRESARGR AKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD 100
SNYVPGWDCH GLPIEWKIEE ENYRSKGKPK PNFKDSAAII AFRKECRAYA 150
DKWLNVQREE FKRLGVIGDW DHPYATMNYA AEAQIARELM KFAANGTLYR 200
GSKPVMWSVV EKTALAEAEV EYEDYTSDTV WVKFPVKTGD ASTKDASVVI 250
WTTTPWTLPG NRAISFSSKI AYGLYKVTDA PADNWAKTGD LLILADGLAE 300
SVFKQARVVA YEKVSDVGAD VLKASECAHP LQGLAGGYEF TVPLLDGDHV 350
TDDTGTGFVH TAPGHGREDF DIWMHNARAL EARGISSVIP YTVDENGALT 400
EQAPGFTGKR VINDKGEKGD ANEAVIQALI ARGALLARGK LKHQYPHSWR 450
SKKPVIFRNT PQWFIAMDKD IVDDGVAKPG DTLRARALQA ISVTQWVPPA 500
GQNRINGMIS GRPDWVISRQ RAWGVPIAVF IKDKGDGSVE ILQDEIVNQR 550
IAEAFMQEGA DAWYAEGAAE RFLGDRAAEG WRKVDDILDV WFDSGSTHAF 600
VLEDAQNFPG LAGIRRKVDG GADTVMYLEG SDQHRGWFHS SLLESCGTRG 650
RAPYDVVLTH GFTLDEQGRK MSKSLGNTTD PAKVIASSGA DILRLWVCAT 700
DYADDQRIGP EILKNVVETY RKLRNSIRWM LGTLHHYHRD EAVAFADMPE 750
LERLMLHQLA EQSATVRAAY AEFDYKTVVA SLAAFMNTEL SAFYFDIRKD 800
TLYCDPPSSL ARKAALTTID IICDAILKWL APVLSFTADE AWSMYRPDAE 850
PSVHLTLFPL DLGEYRDDAL AKKWTLIRAV RRVVTGALEV ERAAKRIGSS 900
LEASPMIYLP EAFMGDIFDV DWAEICITSN AMVEILRGND TPPADAFRLP 950
ELADVAVVVE RAQGTKCARS WKILSSVGSD AEYPDVSPRD AQALREWKAL 1000
GGAAA 1005
Length:1,005
Mass (Da):111,555
Last modified:July 5, 2004 - v1
Checksum:iD69677F6600805C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX572607 Genomic DNA. Translation: CAE29818.1.
RefSeqiNP_949713.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE29818; CAE29818; RPA4377.
GeneIDi2692544.
KEGGirpa:RPA4377.
PATRICi23293357. VBIRhoPal84835_4592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX572607 Genomic DNA. Translation: CAE29818.1 .
RefSeqi NP_949713.1. NC_005296.1.

3D structure databases

ProteinModelPortali Q6N1M8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 258594.RPA4377.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE29818 ; CAE29818 ; RPA4377 .
GeneIDi 2692544.
KEGGi rpa:RPA4377.
PATRICi 23293357. VBIRhoPal84835_4592.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.
PhylomeDBi Q6N1M8.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiSYI_RHOPA
AccessioniPrimary (citable) accession number: Q6N1M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi