Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei629Aminoacyl-adenylateUniRule annotation1
Binding sitei673ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPAL258594:G1GT2-4502-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RPA4377
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001426 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984541 – 1005Isoleucine--tRNA ligaseAdd BLAST1005

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi258594.RPA4377

Structurei

3D structure databases

ProteinModelPortaliQ6N1M8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 80"HIGH" regionAdd BLAST11
Motifi670 – 674"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I
PhylomeDBiQ6N1M8

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q6N1M8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKPKTDAA QSGNDYSKTL FLPQTEFPMR AGLPQREPEL LKRWEEMDLY
60 70 80 90 100
GKLRESARGR AKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD
110 120 130 140 150
SNYVPGWDCH GLPIEWKIEE ENYRSKGKPK PNFKDSAAII AFRKECRAYA
160 170 180 190 200
DKWLNVQREE FKRLGVIGDW DHPYATMNYA AEAQIARELM KFAANGTLYR
210 220 230 240 250
GSKPVMWSVV EKTALAEAEV EYEDYTSDTV WVKFPVKTGD ASTKDASVVI
260 270 280 290 300
WTTTPWTLPG NRAISFSSKI AYGLYKVTDA PADNWAKTGD LLILADGLAE
310 320 330 340 350
SVFKQARVVA YEKVSDVGAD VLKASECAHP LQGLAGGYEF TVPLLDGDHV
360 370 380 390 400
TDDTGTGFVH TAPGHGREDF DIWMHNARAL EARGISSVIP YTVDENGALT
410 420 430 440 450
EQAPGFTGKR VINDKGEKGD ANEAVIQALI ARGALLARGK LKHQYPHSWR
460 470 480 490 500
SKKPVIFRNT PQWFIAMDKD IVDDGVAKPG DTLRARALQA ISVTQWVPPA
510 520 530 540 550
GQNRINGMIS GRPDWVISRQ RAWGVPIAVF IKDKGDGSVE ILQDEIVNQR
560 570 580 590 600
IAEAFMQEGA DAWYAEGAAE RFLGDRAAEG WRKVDDILDV WFDSGSTHAF
610 620 630 640 650
VLEDAQNFPG LAGIRRKVDG GADTVMYLEG SDQHRGWFHS SLLESCGTRG
660 670 680 690 700
RAPYDVVLTH GFTLDEQGRK MSKSLGNTTD PAKVIASSGA DILRLWVCAT
710 720 730 740 750
DYADDQRIGP EILKNVVETY RKLRNSIRWM LGTLHHYHRD EAVAFADMPE
760 770 780 790 800
LERLMLHQLA EQSATVRAAY AEFDYKTVVA SLAAFMNTEL SAFYFDIRKD
810 820 830 840 850
TLYCDPPSSL ARKAALTTID IICDAILKWL APVLSFTADE AWSMYRPDAE
860 870 880 890 900
PSVHLTLFPL DLGEYRDDAL AKKWTLIRAV RRVVTGALEV ERAAKRIGSS
910 920 930 940 950
LEASPMIYLP EAFMGDIFDV DWAEICITSN AMVEILRGND TPPADAFRLP
960 970 980 990 1000
ELADVAVVVE RAQGTKCARS WKILSSVGSD AEYPDVSPRD AQALREWKAL

GGAAA
Length:1,005
Mass (Da):111,555
Last modified:July 5, 2004 - v1
Checksum:iD69677F6600805C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572607 Genomic DNA Translation: CAE29818.1
RefSeqiWP_011159911.1, NC_005296.1

Genome annotation databases

EnsemblBacteriaiCAE29818; CAE29818; RPA4377
KEGGirpa:RPA4377

Similar proteinsi

Entry informationi

Entry nameiSYI_RHOPA
AccessioniPrimary (citable) accession number: Q6N1M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health