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Q6N1M8

- SYI_RHOPA

UniProt

Q6N1M8 - SYI_RHOPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei629 – 6291Aminoacyl-adenylateUniRule annotation
    Binding sitei673 – 6731ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:RPA4377
    OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
    Taxonomic identifieri258594 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
    ProteomesiUP000001426: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10051005Isoleucine--tRNA ligasePRO_0000098454Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi258594.RPA4377.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6N1M8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi70 – 8011"HIGH" regionAdd
    BLAST
    Motifi670 – 6745"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ6N1M8.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6N1M8-1 [UniParc]FASTAAdd to Basket

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    MSDKPKTDAA QSGNDYSKTL FLPQTEFPMR AGLPQREPEL LKRWEEMDLY     50
    GKLRESARGR AKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD 100
    SNYVPGWDCH GLPIEWKIEE ENYRSKGKPK PNFKDSAAII AFRKECRAYA 150
    DKWLNVQREE FKRLGVIGDW DHPYATMNYA AEAQIARELM KFAANGTLYR 200
    GSKPVMWSVV EKTALAEAEV EYEDYTSDTV WVKFPVKTGD ASTKDASVVI 250
    WTTTPWTLPG NRAISFSSKI AYGLYKVTDA PADNWAKTGD LLILADGLAE 300
    SVFKQARVVA YEKVSDVGAD VLKASECAHP LQGLAGGYEF TVPLLDGDHV 350
    TDDTGTGFVH TAPGHGREDF DIWMHNARAL EARGISSVIP YTVDENGALT 400
    EQAPGFTGKR VINDKGEKGD ANEAVIQALI ARGALLARGK LKHQYPHSWR 450
    SKKPVIFRNT PQWFIAMDKD IVDDGVAKPG DTLRARALQA ISVTQWVPPA 500
    GQNRINGMIS GRPDWVISRQ RAWGVPIAVF IKDKGDGSVE ILQDEIVNQR 550
    IAEAFMQEGA DAWYAEGAAE RFLGDRAAEG WRKVDDILDV WFDSGSTHAF 600
    VLEDAQNFPG LAGIRRKVDG GADTVMYLEG SDQHRGWFHS SLLESCGTRG 650
    RAPYDVVLTH GFTLDEQGRK MSKSLGNTTD PAKVIASSGA DILRLWVCAT 700
    DYADDQRIGP EILKNVVETY RKLRNSIRWM LGTLHHYHRD EAVAFADMPE 750
    LERLMLHQLA EQSATVRAAY AEFDYKTVVA SLAAFMNTEL SAFYFDIRKD 800
    TLYCDPPSSL ARKAALTTID IICDAILKWL APVLSFTADE AWSMYRPDAE 850
    PSVHLTLFPL DLGEYRDDAL AKKWTLIRAV RRVVTGALEV ERAAKRIGSS 900
    LEASPMIYLP EAFMGDIFDV DWAEICITSN AMVEILRGND TPPADAFRLP 950
    ELADVAVVVE RAQGTKCARS WKILSSVGSD AEYPDVSPRD AQALREWKAL 1000
    GGAAA 1005
    Length:1,005
    Mass (Da):111,555
    Last modified:July 5, 2004 - v1
    Checksum:iD69677F6600805C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX572607 Genomic DNA. Translation: CAE29818.1.
    RefSeqiNP_949713.1. NC_005296.1.

    Genome annotation databases

    EnsemblBacteriaiCAE29818; CAE29818; RPA4377.
    GeneIDi2692544.
    KEGGirpa:RPA4377.
    PATRICi23293357. VBIRhoPal84835_4592.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX572607 Genomic DNA. Translation: CAE29818.1 .
    RefSeqi NP_949713.1. NC_005296.1.

    3D structure databases

    ProteinModelPortali Q6N1M8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 258594.RPA4377.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE29818 ; CAE29818 ; RPA4377 .
    GeneIDi 2692544.
    KEGGi rpa:RPA4377.
    PATRICi 23293357. VBIRhoPal84835_4592.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q6N1M8.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-98 / CGA009.

    Entry informationi

    Entry nameiSYI_RHOPA
    AccessioniPrimary (citable) accession number: Q6N1M8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3