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Q6N1M8

- SYI_RHOPA

UniProt

Q6N1M8 - SYI_RHOPA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei629 – 6291Aminoacyl-adenylateUniRule annotation
Binding sitei673 – 6731ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RPA4377
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10051005Isoleucine--tRNA ligasePRO_0000098454Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi258594.RPA4377.

Structurei

3D structure databases

ProteinModelPortaliQ6N1M8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8011"HIGH" regionAdd
BLAST
Motifi670 – 6745"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.
PhylomeDBiQ6N1M8.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6N1M8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDKPKTDAA QSGNDYSKTL FLPQTEFPMR AGLPQREPEL LKRWEEMDLY
60 70 80 90 100
GKLRESARGR AKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD
110 120 130 140 150
SNYVPGWDCH GLPIEWKIEE ENYRSKGKPK PNFKDSAAII AFRKECRAYA
160 170 180 190 200
DKWLNVQREE FKRLGVIGDW DHPYATMNYA AEAQIARELM KFAANGTLYR
210 220 230 240 250
GSKPVMWSVV EKTALAEAEV EYEDYTSDTV WVKFPVKTGD ASTKDASVVI
260 270 280 290 300
WTTTPWTLPG NRAISFSSKI AYGLYKVTDA PADNWAKTGD LLILADGLAE
310 320 330 340 350
SVFKQARVVA YEKVSDVGAD VLKASECAHP LQGLAGGYEF TVPLLDGDHV
360 370 380 390 400
TDDTGTGFVH TAPGHGREDF DIWMHNARAL EARGISSVIP YTVDENGALT
410 420 430 440 450
EQAPGFTGKR VINDKGEKGD ANEAVIQALI ARGALLARGK LKHQYPHSWR
460 470 480 490 500
SKKPVIFRNT PQWFIAMDKD IVDDGVAKPG DTLRARALQA ISVTQWVPPA
510 520 530 540 550
GQNRINGMIS GRPDWVISRQ RAWGVPIAVF IKDKGDGSVE ILQDEIVNQR
560 570 580 590 600
IAEAFMQEGA DAWYAEGAAE RFLGDRAAEG WRKVDDILDV WFDSGSTHAF
610 620 630 640 650
VLEDAQNFPG LAGIRRKVDG GADTVMYLEG SDQHRGWFHS SLLESCGTRG
660 670 680 690 700
RAPYDVVLTH GFTLDEQGRK MSKSLGNTTD PAKVIASSGA DILRLWVCAT
710 720 730 740 750
DYADDQRIGP EILKNVVETY RKLRNSIRWM LGTLHHYHRD EAVAFADMPE
760 770 780 790 800
LERLMLHQLA EQSATVRAAY AEFDYKTVVA SLAAFMNTEL SAFYFDIRKD
810 820 830 840 850
TLYCDPPSSL ARKAALTTID IICDAILKWL APVLSFTADE AWSMYRPDAE
860 870 880 890 900
PSVHLTLFPL DLGEYRDDAL AKKWTLIRAV RRVVTGALEV ERAAKRIGSS
910 920 930 940 950
LEASPMIYLP EAFMGDIFDV DWAEICITSN AMVEILRGND TPPADAFRLP
960 970 980 990 1000
ELADVAVVVE RAQGTKCARS WKILSSVGSD AEYPDVSPRD AQALREWKAL

GGAAA
Length:1,005
Mass (Da):111,555
Last modified:July 5, 2004 - v1
Checksum:iD69677F6600805C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572607 Genomic DNA. Translation: CAE29818.1.
RefSeqiNP_949713.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE29818; CAE29818; RPA4377.
GeneIDi2692544.
KEGGirpa:RPA4377.
PATRICi23293357. VBIRhoPal84835_4592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572607 Genomic DNA. Translation: CAE29818.1 .
RefSeqi NP_949713.1. NC_005296.1.

3D structure databases

ProteinModelPortali Q6N1M8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 258594.RPA4377.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE29818 ; CAE29818 ; RPA4377 .
GeneIDi 2692544.
KEGGi rpa:RPA4377.
PATRICi 23293357. VBIRhoPal84835_4592.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.
PhylomeDBi Q6N1M8.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiSYI_RHOPA
AccessioniPrimary (citable) accession number: Q6N1M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3