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Protein

Molybdate-anion transporter

Gene

MFSD5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates high-affinity intracellular uptake of the rare oligo-element molybdenum.1 Publication

Kineticsi

  1. KM=546 nM for molybdate (when expressed in yeast)1 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Molybdate-anion transporter
    Alternative name(s):
    Major facilitator superfamily domain-containing protein 5
    Molybdate transporter 2 homolog
    Short name:
    hsMOT2
    Gene namesi
    Name:MFSD5
    ORF Names:UNQ832/PRO1759
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:28156. MFSD5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei43 – 6321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei79 – 9921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei128 – 14821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei249 – 26921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei409 – 42921HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: UniProtKB
    • plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671466.

    Polymorphism and mutation databases

    BioMutaiMFSD5.
    DMDMi124015161.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 450450Molybdate-anion transporterPRO_0000273401Add
    BLAST

    Proteomic databases

    MaxQBiQ6N075.
    PaxDbiQ6N075.
    PRIDEiQ6N075.

    PTM databases

    PhosphoSiteiQ6N075.

    Expressioni

    Tissue specificityi

    Expressed ubiquitously but at relatively higher levels in the olfactory bulb and the skeletal muscle.1 Publication

    Gene expression databases

    BgeeiQ6N075.
    CleanExiHS_MFSD5.
    ExpressionAtlasiQ6N075. baseline and differential.
    GenevisibleiQ6N075. HS.

    Organism-specific databases

    HPAiHPA039773.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCDC155Q8N6L03EBI-3920969,EBI-749265
    CREB3L1Q96BA83EBI-3920969,EBI-6942903

    Protein-protein interaction databases

    BioGridi124404. 6 interactions.
    IntActiQ6N075. 5 interactions.
    MINTiMINT-4724121.
    STRINGi9606.ENSP00000442688.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6N075.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the major facilitator superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG309381.
    GeneTreeiENSGT00390000012629.
    HOGENOMiHOG000239662.
    HOVERGENiHBG081970.
    InParanoidiQ6N075.
    OMAiFESVLYI.
    OrthoDBiEOG71ZP22.
    PhylomeDBiQ6N075.
    TreeFamiTF328562.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR030163. MFSD5.
    IPR008509. MOT2.
    [Graphical view]
    PANTHERiPTHR24003:SF500. PTHR24003:SF500. 1 hit.
    PfamiPF05631. DUF791. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q6N075-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MLVTAYLAFV GLLASCLGLE LSRCRAKPPG RACSNPSFLR FQLDFYQVYF
    60 70 80 90 100
    LALAADWLQA PYLYKLYQHY YFLEGQIAIL YVCGLASTVL FGLVASSLVD
    110 120 130 140 150
    WLGRKNSCVL FSLTYSLCCL TKLSQDYFVL LVGRALGGLS TALLFSAFEA
    160 170 180 190 200
    WYIHEHVERH DFPAEWIPAT FARAAFWNHV LAVVAGVAAE AVASWIGLGP
    210 220 230 240 250
    VAPFVAAIPL LALAGALALR NWGENYDRQR AFSRTCAGGL RCLLSDRRVL
    260 270 280 290 300
    LLGTIQALFE SVIFIFVFLW TPVLDPHGAP LGIIFSSFMA ASLLGSSLYR
    310 320 330 340 350
    IATSKRYHLQ PMHLLSLAVL IVVFSLFMLT FSTSPGQESP VESFIAFLLI
    360 370 380 390 400
    ELACGLYFPS MSFLRRKVIP ETEQAGVLNW FRVPLHSLAC LGLLVLHDSD
    410 420 430 440 450
    RKTGTRNMFS ICSAVMVMAL LAVVGLFTVV RHDAELRVPS PTEEPYAPEL
    Length:450
    Mass (Da):49,765
    Last modified:January 23, 2007 - v2
    Checksum:iA15D005755941BA7
    GO
    Isoform 2 (identifier: Q6N075-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MEVTAVGEEA...RARVVRGPTM

    Note: No experimental confirmation available.
    Show »
    Length:557
    Mass (Da):61,504
    Checksum:i85CD96E4BF023226
    GO

    Sequence cautioni

    The sequence BAC05108.1 differs from that shown.Probable cloning artifact.Curated
    The sequence CAE45795.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti130 – 1301L → M in AAH67795 (PubMed:16541075).Curated
    Sequence conflicti157 – 1571V → A in BAC11137 (PubMed:16303743).Curated
    Sequence conflicti425 – 4251G → E in CAE45795 (PubMed:17974005).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MEVTAVGEEANQGDLLGFGQ RCCSLGSSNPWLYRTAGIFP EHCTEEKKIGTYFRVAPQAS KSQRSRCHGMAASEARPGEA TAASYGRPFPTHPRRQEFRA RVVRGPTM in isoform 2. CuratedVSP_046647

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY358604 mRNA. Translation: AAQ88967.1.
    BX640649 mRNA. Translation: CAE45795.1. Different initiation.
    AK097576 mRNA. Translation: BAC05108.1. Sequence problems.
    AK074684 mRNA. Translation: BAC11137.1.
    AC021072 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW96679.1.
    BC007703 mRNA. Translation: AAH07703.1.
    BC067795 mRNA. Translation: AAH67795.1.
    CCDSiCCDS53796.1. [Q6N075-2]
    CCDS8851.1. [Q6N075-1]
    RefSeqiNP_001164261.1. NM_001170790.1. [Q6N075-2]
    NP_116278.3. NM_032889.4. [Q6N075-1]
    XP_005269254.1. XM_005269197.1. [Q6N075-2]
    XP_005269255.1. XM_005269198.3. [Q6N075-1]
    UniGeneiHs.654660.

    Genome annotation databases

    EnsembliENST00000329548; ENSP00000332624; ENSG00000182544.
    ENST00000534842; ENSP00000442688; ENSG00000182544. [Q6N075-2]
    GeneIDi84975.
    KEGGihsa:84975.
    UCSCiuc001sch.2. human.
    uc001sci.2. human. [Q6N075-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY358604 mRNA. Translation: AAQ88967.1.
    BX640649 mRNA. Translation: CAE45795.1. Different initiation.
    AK097576 mRNA. Translation: BAC05108.1. Sequence problems.
    AK074684 mRNA. Translation: BAC11137.1.
    AC021072 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW96679.1.
    BC007703 mRNA. Translation: AAH07703.1.
    BC067795 mRNA. Translation: AAH67795.1.
    CCDSiCCDS53796.1. [Q6N075-2]
    CCDS8851.1. [Q6N075-1]
    RefSeqiNP_001164261.1. NM_001170790.1. [Q6N075-2]
    NP_116278.3. NM_032889.4. [Q6N075-1]
    XP_005269254.1. XM_005269197.1. [Q6N075-2]
    XP_005269255.1. XM_005269198.3. [Q6N075-1]
    UniGeneiHs.654660.

    3D structure databases

    ProteinModelPortaliQ6N075.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi124404. 6 interactions.
    IntActiQ6N075. 5 interactions.
    MINTiMINT-4724121.
    STRINGi9606.ENSP00000442688.

    PTM databases

    PhosphoSiteiQ6N075.

    Polymorphism and mutation databases

    BioMutaiMFSD5.
    DMDMi124015161.

    Proteomic databases

    MaxQBiQ6N075.
    PaxDbiQ6N075.
    PRIDEiQ6N075.

    Protocols and materials databases

    DNASUi84975.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000329548; ENSP00000332624; ENSG00000182544.
    ENST00000534842; ENSP00000442688; ENSG00000182544. [Q6N075-2]
    GeneIDi84975.
    KEGGihsa:84975.
    UCSCiuc001sch.2. human.
    uc001sci.2. human. [Q6N075-1]

    Organism-specific databases

    CTDi84975.
    GeneCardsiGC12P053645.
    HGNCiHGNC:28156. MFSD5.
    HPAiHPA039773.
    neXtProtiNX_Q6N075.
    PharmGKBiPA142671466.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG309381.
    GeneTreeiENSGT00390000012629.
    HOGENOMiHOG000239662.
    HOVERGENiHBG081970.
    InParanoidiQ6N075.
    OMAiFESVLYI.
    OrthoDBiEOG71ZP22.
    PhylomeDBiQ6N075.
    TreeFamiTF328562.

    Miscellaneous databases

    GenomeRNAii84975.
    NextBioi75529.
    PROiQ6N075.

    Gene expression databases

    BgeeiQ6N075.
    CleanExiHS_MFSD5.
    ExpressionAtlasiQ6N075. baseline and differential.
    GenevisibleiQ6N075. HS.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR030163. MFSD5.
    IPR008509. MOT2.
    [Graphical view]
    PANTHERiPTHR24003:SF500. PTHR24003:SF500. 1 hit.
    PfamiPF05631. DUF791. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon endothelium.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Mammary gland.
    5. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain and Ovary.
    8. "Long evolutionary conservation and considerable tissue specificity of several atypical solute carrier transporters."
      Sreedharan S., Stephansson O., Schioth H.B., Fredriksson R.
      Gene 478:11-18(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiMFSD5_HUMAN
    AccessioniPrimary (citable) accession number: Q6N075
    Secondary accession number(s): G3V1N7
    , Q6NW04, Q8N7W8, Q8NCK0, Q96IA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: January 23, 2007
    Last modified: July 22, 2015
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.