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Q6N075 (MFSD5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Molybdate-anion transporter
Alternative name(s):
Major facilitator superfamily domain-containing protein 5
Molybdate transporter 2 homolog
Short name=hsMOT2
Gene names
Name:MFSD5
ORF Names:UNQ832/PRO1759
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates high-affinity intracellular uptake of the rare oligo-element molybdenum. Ref.9

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed ubiquitously but at relatively higher levels in the olfactory bulb and the skeletal muscle. Ref.8

Sequence similarities

Belongs to the major facilitator superfamily.

Biophysicochemical properties

Kinetic parameters:

KM=546 nM for molybdate (when expressed in yeast) Ref.9

Sequence caution

The sequence BAC05108.1 differs from that shown. Reason: Probable cloning artifact.

The sequence CAE45795.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_procession transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6N075-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6N075-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MEVTAVGEEA...RARVVRGPTM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Molybdate-anion transporter
PRO_0000273401

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane43 – 6321Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane195 – 21521Helical; Potential
Transmembrane249 – 26921Helical; Potential
Transmembrane278 – 29821Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane344 – 36421Helical; Potential
Transmembrane376 – 39621Helical; Potential
Transmembrane409 – 42921Helical; Potential

Natural variations

Alternative sequence11M → MEVTAVGEEANQGDLLGFGQ RCCSLGSSNPWLYRTAGIFP EHCTEEKKIGTYFRVAPQAS KSQRSRCHGMAASEARPGEA TAASYGRPFPTHPRRQEFRA RVVRGPTM in isoform 2.
VSP_046647

Experimental info

Sequence conflict1301L → M in AAH67795. Ref.5
Sequence conflict1571V → A in BAC11137. Ref.4
Sequence conflict4251G → E in CAE45795. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A15D005755941BA7

FASTA45049,765
        10         20         30         40         50         60 
MLVTAYLAFV GLLASCLGLE LSRCRAKPPG RACSNPSFLR FQLDFYQVYF LALAADWLQA 

        70         80         90        100        110        120 
PYLYKLYQHY YFLEGQIAIL YVCGLASTVL FGLVASSLVD WLGRKNSCVL FSLTYSLCCL 

       130        140        150        160        170        180 
TKLSQDYFVL LVGRALGGLS TALLFSAFEA WYIHEHVERH DFPAEWIPAT FARAAFWNHV 

       190        200        210        220        230        240 
LAVVAGVAAE AVASWIGLGP VAPFVAAIPL LALAGALALR NWGENYDRQR AFSRTCAGGL 

       250        260        270        280        290        300 
RCLLSDRRVL LLGTIQALFE SVIFIFVFLW TPVLDPHGAP LGIIFSSFMA ASLLGSSLYR 

       310        320        330        340        350        360 
IATSKRYHLQ PMHLLSLAVL IVVFSLFMLT FSTSPGQESP VESFIAFLLI ELACGLYFPS 

       370        380        390        400        410        420 
MSFLRRKVIP ETEQAGVLNW FRVPLHSLAC LGLLVLHDSD RKTGTRNMFS ICSAVMVMAL 

       430        440        450 
LAVVGLFTVV RHDAELRVPS PTEEPYAPEL 

« Hide

Isoform 2 [UniParc].

Checksum: 85CD96E4BF023226
Show »

FASTA55761,504

References

« Hide 'large scale' references
[1]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Colon endothelium.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary gland.
[5]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Ovary.
[8]"Long evolutionary conservation and considerable tissue specificity of several atypical solute carrier transporters."
Sreedharan S., Stephansson O., Schioth H.B., Fredriksson R.
Gene 478:11-18(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Algae and humans share a molybdate transporter."
Tejada-Jimenez M., Galvan A., Fernandez E.
Proc. Natl. Acad. Sci. U.S.A. 108:6420-6425(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY358604 mRNA. Translation: AAQ88967.1.
BX640649 mRNA. Translation: CAE45795.1. Different initiation.
AK097576 mRNA. Translation: BAC05108.1. Sequence problems.
AK074684 mRNA. Translation: BAC11137.1.
AC021072 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96679.1.
BC007703 mRNA. Translation: AAH07703.1.
BC067795 mRNA. Translation: AAH67795.1.
RefSeqNP_001164261.1. NM_001170790.1.
NP_116278.3. NM_032889.4.
XP_005269254.1. XM_005269197.1.
XP_005269255.1. XM_005269198.2.
UniGeneHs.654660.

3D structure databases

ProteinModelPortalQ6N075.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124404. 4 interactions.
IntActQ6N075. 3 interactions.
MINTMINT-4724121.
STRING9606.ENSP00000332624.

PTM databases

PhosphoSiteQ6N075.

Polymorphism databases

DMDM124015161.

Proteomic databases

PaxDbQ6N075.
PRIDEQ6N075.

Protocols and materials databases

DNASU84975.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000329548; ENSP00000332624; ENSG00000182544. [Q6N075-1]
ENST00000534842; ENSP00000442688; ENSG00000182544. [Q6N075-2]
GeneID84975.
KEGGhsa:84975.
UCSCuc001sch.2. human.
uc001sci.2. human. [Q6N075-1]

Organism-specific databases

CTD84975.
GeneCardsGC12P053645.
HGNCHGNC:28156. MFSD5.
HPAHPA039773.
neXtProtNX_Q6N075.
PharmGKBPA142671466.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309381.
HOGENOMHOG000239662.
HOVERGENHBG081970.
InParanoidQ6N075.
OMAFPKEWIP.
OrthoDBEOG71ZP22.
PhylomeDBQ6N075.
TreeFamTF328562.

Gene expression databases

ArrayExpressQ6N075.
BgeeQ6N075.
CleanExHS_MFSD5.
GenevestigatorQ6N075.

Family and domain databases

InterProIPR008509. DUF791.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF05631. DUF791. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi84975.
NextBio75529.
PROQ6N075.

Entry information

Entry nameMFSD5_HUMAN
AccessionPrimary (citable) accession number: Q6N075
Secondary accession number(s): G3V1N7 expand/collapse secondary AC list , Q6NW04, Q8N7W8, Q8NCK0, Q96IA5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM