Q6N063 (OGFD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
EC=1.14.11.-

Gene names

Name:

OGFOD2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Cofactor	Binds 1 Fe2+ ion per subunit By similarity. Ascorbate By similarity.
Sequence similarities	Belongs to the OGFOD2 family. Contains 1 Fe2OG dioxygenase domain.
Sequence caution	The sequence CAE45849.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame. The sequence CAE45849.1 differs from that shown. Reason: Frameshift at position 104.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6N063-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6N063-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.
     61-62: LA → MV

Isoform 3 (identifier: Q6N063-3)

The sequence of this isoform differs from the canonical sequence as follows:
     64-64: L → WPQEDGRPHLVTTLCHPSLSRRWSGGSGWGRSQQLGKPSSRVPTTRHGLRSTTHCRMQLWPPSSWP
     65-350: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q6N063-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-164: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2		PRO_0000288978
Regions
	Domain	215 – 309	95	Fe2OG dioxygenase
Sites
	Metal binding	235	1	Iron By similarity
	Metal binding	237	1	Iron By similarity
	Metal binding	290	1	Iron By similarity
	Binding site	300	1	2-oxoglutarate Potential
Natural variations
	Alternative sequence	1 – 164	164	Missing in isoform 4.		VSP_025855
	Alternative sequence	1 – 60	60	Missing in isoform 2.		VSP_025856
	Alternative sequence	61 – 62	2	LA → MV in isoform 2.		VSP_025857
	Alternative sequence	64	1	L → WPQEDGRPHLVTTLCHPSLS RRWSGGSGWGRSQQLGKPSS RVPTTRHGLRSTTHCRMQLW PPSSWP in isoform 3.		VSP_025858
	Alternative sequence	65 – 350	286	Missing in isoform 3.		VSP_025859
Experimental info
	Sequence conflict	244	1	N → S in CAE45807. Ref.2
	Sequence conflict	311	1	A → T in AAH98119. Ref.5
	Sequence conflict	311	1	A → T in AAI01941. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 651D0F07A32EBB66	FASTA	350	38,996
	10 20 30 40 50 60 MATVGAPRHF CRCACFCTDN LYVARYGLHV RFRGEQQLRR DYGPILRSRG CVSAKDFQQL 70 80 90 100 110 120 LAELEQEVER RQRLGQESAA RKALIASSYH PARPEVYDSL QDAALAPEFL AVTEYSVSPD 130 140 150 160 170 180 ADLKGLLQRL ETVSEEKRIY RVPVFTAPFC QALLEELEHF EQSDMPKGRP NTMNNYGVLL 190 200 210 220 230 240 HELGLDEPLM TPLRERFLQP LMALLYPDCG GGRLDSHRAF VVKYAPGQDL ELGCHYDNAE 250 260 270 280 290 300 LTLNVALGKV FTGGALYFGG LFQAPTALTE PLEVEHVVGQ GVLHRGGQLH GARPLGTGER 310 320 330 340 350 WNLVVWLRAS AVRNSLCPMC CREPDLVDDE GFGDGFTREE PATVDVCALT
	Isoform 2 [UniParc]. Checksum: 5B5A06579ED4EDAD	FASTA	290	32,121
	10 20 30 40 50 60 MVELEQEVER RQRLGQESAA RKALIASSYH PARPEVYDSL QDAALAPEFL AVTEYSVSPD 70 80 90 100 110 120 ADLKGLLQRL ETVSEEKRIY RVPVFTAPFC QALLEELEHF EQSDMPKGRP NTMNNYGVLL 130 140 150 160 170 180 HELGLDEPLM TPLRERFLQP LMALLYPDCG GGRLDSHRAF VVKYAPGQDL ELGCHYDNAE 190 200 210 220 230 240 LTLNVALGKV FTGGALYFGG LFQAPTALTE PLEVEHVVGQ GVLHRGGQLH GARPLGTGER 250 260 270 280 290 WNLVVWLRAS AVRNSLCPMC CREPDLVDDE GFGDGFTREE PATVDVCALT
	Isoform 3 [UniParc]. Checksum: C94B1F86CA7B53CD	FASTA	129	14,735
	10 20 30 40 50 60 MATVGAPRHF CRCACFCTDN LYVARYGLHV RFRGEQQLRR DYGPILRSRG CVSAKDFQQL 70 80 90 100 110 120 LAEWPQEDGR PHLVTTLCHP SLSRRWSGGS GWGRSQQLGK PSSRVPTTRH GLRSTTHCRM QLWPPSSWP
	Isoform 4 [UniParc]. Checksum: 5D2FAFD3CEB790F8	FASTA	186	20,288
	10 20 30 40 50 60 MPKGRPNTMN NYGVLLHELG LDEPLMTPLR ERFLQPLMAL LYPDCGGGRL DSHRAFVVKY 70 80 90 100 110 120 APGQDLELGC HYDNAELTLN VALGKVFTGG ALYFGGLFQA PTALTEPLEV EHVVGQGVLH 130 140 150 160 170 180 RGGQLHGARP LGTGERWNLV VWLRASAVRN SLCPMCCREP DLVDDEGFGD GFTREEPATV DVCALT

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Placenta.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Colon endothelium and Fetal liver.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK023553 mRNA. Translation: BAB14610.1. AK094820 mRNA. Translation: BAG52936.1. CR457316 mRNA. Translation: CAG33597.1. BX640675 mRNA. Translation: CAE45807.1. BX640734 mRNA. Translation: CAE45849.1. Sequence problems. CH471054 Genomic DNA. Translation: EAW98364.1. BC098119 mRNA. Translation: AAH98119.1. BC098293 mRNA. Translation: AAH98293.1. BC101940 mRNA. Translation: AAI01941.1. BC105637 mRNA. Translation: AAI05638.1.
IPI	IPI00002458. IPI00794111. IPI00795704. IPI00847506.
RefSeq	NP_078899.1. NM_024623.1.
UniGene	Hs.524817.
3D structure databases
ProteinModelPortal	Q6N063.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6N063. 2 interactions.
MINT	MINT-1407968.
STRING	9606.ENSP00000380544.
PTM databases
PhosphoSite	Q6N063.
Polymorphism databases
DMDM	156633666.
Proteomic databases
PaxDb	Q6N063.
PRIDE	Q6N063.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000228922; ENSP00000228922; ENSG00000111325. ENST00000397389; ENSP00000380544; ENSG00000111325. ENST00000454694; ENSP00000394175; ENSG00000111325. ENST00000536150; ENSP00000438327; ENSG00000111325. ENST00000538628; ENSP00000444608; ENSG00000111325. ENST00000538755; ENSP00000442817; ENSG00000111325. ENST00000540324; ENSP00000439667; ENSG00000111325. ENST00000545317; ENSP00000438192; ENSG00000111325. ENST00000545612; ENSP00000444436; ENSG00000111325.
GeneID	79676.
KEGG	hsa:79676.
UCSC	uc001uds.1. human. uc001udz.1. human.
Organism-specific databases
CTD	79676.
GeneCards	GC12P123459.
H-InvDB	HIX0079485.
HGNC	HGNC:25823. OGFOD2.
HPA	HPA039448. HPA040306.
neXtProt	NX_Q6N063.
PharmGKB	PA143485569.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG248193.
HOGENOM	HOG000008117.
HOVERGEN	HBG108212.
InParanoid	Q6N063.
OMA	RFLQPLM.
OrthoDB	EOG4KKZ3D.
Gene expression databases
ArrayExpress	Q6N063.
Bgee	Q6N063.
CleanEx	HS_OGFOD2.
Genevestigator	Q6N063.
Family and domain databases
InterPro	IPR005123. Oxoglu/Fe-dep_dioxygenase. IPR006620. Pro_4_hyd_alph. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	OGFOD2. human.
DrugBank	DB00126. Vitamin C.
GenomeRNAi	79676.
NextBio	68918.

Entry information

Entry name

OGFD2_HUMAN

Accession

Primary (citable) accession number: Q6N063
Secondary accession number(s): B3KT24 , Q4KN13, Q6N023, Q9H8K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.