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Protein

Calpain-13

Gene

CAPN13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable non-lysosomal thiol-protease.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93PROSITE-ProRule annotation1
Active sitei249PROSITE-ProRule annotation1
Active sitei273PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-13 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 13
Short name:
CANP 13
Gene namesi
Name:CAPN13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:16663. CAPN13.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi92291.
OpenTargetsiENSG00000162949.
PharmGKBiPA134931170.

Polymorphism and mutation databases

BioMutaiCAPN13.
DMDMi117949375.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002595821 – 669Calpain-13Add BLAST669

Proteomic databases

EPDiQ6MZZ7.
MaxQBiQ6MZZ7.
PaxDbiQ6MZZ7.
PeptideAtlasiQ6MZZ7.
PRIDEiQ6MZZ7.

PTM databases

iPTMnetiQ6MZZ7.
PhosphoSitePlusiQ6MZZ7.

Expressioni

Tissue specificityi

Weakly expressed in lung and testis. Weakly or not expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSG00000162949.
CleanExiHS_CAPN13.
ExpressionAtlasiQ6MZZ7. baseline and differential.
GenevisibleiQ6MZZ7. HS.

Organism-specific databases

HPAiHPA029497.
HPA030034.

Interactioni

Protein-protein interaction databases

BioGridi124928. 12 interactors.
STRINGi9606.ENSP00000295055.

Structurei

Secondary structure

1669
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi518 – 529Combined sources12
Helixi540 – 550Combined sources11
Beta strandi555 – 558Combined sources4
Helixi560 – 580Combined sources21
Beta strandi586 – 588Combined sources3
Helixi589 – 591Combined sources3
Helixi592 – 597Combined sources6
Helixi600 – 602Combined sources3
Helixi609 – 619Combined sources11
Beta strandi624 – 626Combined sources3
Helixi628 – 648Combined sources21
Beta strandi650 – 653Combined sources4
Helixi659 – 667Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I7AX-ray1.80A515-669[»]
ProteinModelPortaliQ6MZZ7.
SMRiQ6MZZ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6MZZ7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 333Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini538 – 573EF-hand 1Add BLAST36
Domaini636 – 669EF-hand 2Add BLAST34

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
KOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ6MZZ7.
KOiK08581.
OMAiLTEMEWM.
OrthoDBiEOG091G03KK.
PhylomeDBiQ6MZZ7.
TreeFamiTF314748.

Family and domain databases

CDDicd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR029544. CANP13/Calpain-C.
IPR011992. EF-hand-dom_pair.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF282. PTHR10183:SF282. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6MZZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK
60 70 80 90 100
LLQEKRLSNV IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG
110 120 130 140 150
SLTQNPQYRQ KILMVQSFSH QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD
160 170 180 190 200
KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY SDLHYGFLED ALVDLTGGVI
210 220 230 240 250
TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA MENGLVSLHA
260 270 280 290 300
YTVTGAEQIQ YRRGWEEIIS LWNPWGWGEA EWRGRWSDGS QEWEETCDPR
310 320 330 340 350
KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI
360 370 380 390 400
MFRKQVILGN TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE
410 420 430 440 450
DAKFPLDFQV ILAGSQRFRE KFPPVFFSSF RNTVQSSNNK FRRNFTMTYH
460 470 480 490 500
LSPGNYVVVA QTRRKSAEFL LRIFLKMPDS DRHLSSHFNL RMKGSPSEHG
510 520 530 540 550
SQQSIFNRYA QQRLDIDATQ LQGLLNQELL TGPPGDMFSL DECRSLVALM
560 570 580 590 600
ELKVNGRLDQ EEFARLWKRL VHYQHVFQKV QTSPGVLLSS DLWKAIENTD
610 620 630 640 650
FLRGIFISRE LLHLVTLRYS DSVGRVSFPS LVCFLMRLEA MAKTFRNLSK
660
DGKGLYLTEM EWMSLVMYN
Length:669
Mass (Da):76,696
Last modified:October 31, 2006 - v2
Checksum:iBB24CF09D55CF790
GO
Isoform 2 (identifier: Q6MZZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     417-423: RFREKFP → KHCPKLK
     424-669: Missing.

Note: No experimental confirmation available.
Show »
Length:423
Mass (Da):48,002
Checksum:i54122B6EFF505BCC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti562E → G in CAE45878 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028964249H → Y.Corresponds to variant rs17010210dbSNPEnsembl.1
Natural variantiVAR_028965280A → T.2 PublicationsCorresponds to variant rs508405dbSNPEnsembl.1
Natural variantiVAR_028966596I → T.Corresponds to variant rs2276568dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021476417 – 423RFREKFP → KHCPKLK in isoform 2. 1 Publication7
Alternative sequenceiVSP_021477424 – 669Missing in isoform 2. 1 PublicationAdd BLAST246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074418 mRNA. Translation: BAB85075.1.
BX640793 mRNA. Translation: CAE45878.1.
AC092569 Genomic DNA. Translation: AAX82015.1.
BC117347 mRNA. Translation: AAI17348.1.
BC117345 mRNA. Translation: AAI17346.1.
CCDSiCCDS46252.1. [Q6MZZ7-1]
RefSeqiNP_653176.2. NM_144575.2. [Q6MZZ7-1]
XP_011531461.1. XM_011533159.2. [Q6MZZ7-1]
XP_011531462.1. XM_011533160.2. [Q6MZZ7-1]
XP_011531463.1. XM_011533161.2. [Q6MZZ7-1]
XP_016860753.1. XM_017005264.1. [Q6MZZ7-1]
XP_016860754.1. XM_017005265.1. [Q6MZZ7-1]
UniGeneiHs.660911.

Genome annotation databases

EnsembliENST00000295055; ENSP00000295055; ENSG00000162949. [Q6MZZ7-1]
ENST00000458085; ENSP00000416191; ENSG00000162949. [Q6MZZ7-2]
GeneIDi92291.
KEGGihsa:92291.
UCSCiuc061hvv.1. human. [Q6MZZ7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074418 mRNA. Translation: BAB85075.1.
BX640793 mRNA. Translation: CAE45878.1.
AC092569 Genomic DNA. Translation: AAX82015.1.
BC117347 mRNA. Translation: AAI17348.1.
BC117345 mRNA. Translation: AAI17346.1.
CCDSiCCDS46252.1. [Q6MZZ7-1]
RefSeqiNP_653176.2. NM_144575.2. [Q6MZZ7-1]
XP_011531461.1. XM_011533159.2. [Q6MZZ7-1]
XP_011531462.1. XM_011533160.2. [Q6MZZ7-1]
XP_011531463.1. XM_011533161.2. [Q6MZZ7-1]
XP_016860753.1. XM_017005264.1. [Q6MZZ7-1]
XP_016860754.1. XM_017005265.1. [Q6MZZ7-1]
UniGeneiHs.660911.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I7AX-ray1.80A515-669[»]
ProteinModelPortaliQ6MZZ7.
SMRiQ6MZZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124928. 12 interactors.
STRINGi9606.ENSP00000295055.

Protein family/group databases

MEROPSiC02.020.

PTM databases

iPTMnetiQ6MZZ7.
PhosphoSitePlusiQ6MZZ7.

Polymorphism and mutation databases

BioMutaiCAPN13.
DMDMi117949375.

Proteomic databases

EPDiQ6MZZ7.
MaxQBiQ6MZZ7.
PaxDbiQ6MZZ7.
PeptideAtlasiQ6MZZ7.
PRIDEiQ6MZZ7.

Protocols and materials databases

DNASUi92291.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295055; ENSP00000295055; ENSG00000162949. [Q6MZZ7-1]
ENST00000458085; ENSP00000416191; ENSG00000162949. [Q6MZZ7-2]
GeneIDi92291.
KEGGihsa:92291.
UCSCiuc061hvv.1. human. [Q6MZZ7-1]

Organism-specific databases

CTDi92291.
DisGeNETi92291.
GeneCardsiCAPN13.
H-InvDBHIX0018641.
HGNCiHGNC:16663. CAPN13.
HPAiHPA029497.
HPA030034.
MIMi610228. gene.
neXtProtiNX_Q6MZZ7.
OpenTargetsiENSG00000162949.
PharmGKBiPA134931170.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
KOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ6MZZ7.
KOiK08581.
OMAiLTEMEWM.
OrthoDBiEOG091G03KK.
PhylomeDBiQ6MZZ7.
TreeFamiTF314748.

Enzyme and pathway databases

ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Miscellaneous databases

ChiTaRSiCAPN13. human.
EvolutionaryTraceiQ6MZZ7.
GenomeRNAii92291.
PROiQ6MZZ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162949.
CleanExiHS_CAPN13.
ExpressionAtlasiQ6MZZ7. baseline and differential.
GenevisibleiQ6MZZ7. HS.

Family and domain databases

CDDicd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR029544. CANP13/Calpain-C.
IPR011992. EF-hand-dom_pair.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF282. PTHR10183:SF282. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN13_HUMAN
AccessioniPrimary (citable) accession number: Q6MZZ7
Secondary accession number(s): Q17RF0, Q580X1, Q8TE80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is unlikely that this protein binds calcium as none of the 2 EF-hand domains seem to contain a canonical calcium-binding site.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.