Q6MZZ7 (CAN13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-13
EC=3.4.22.-

Alternative name(s):
Calcium-activated neutral proteinase 13
Short name=CANP 13

Gene names

Name:

CAPN13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable non-lysosomal thiol-protease By similarity.
Tissue specificity	Weakly expressed in lung and testis. Weakly or not expressed in other tissues. Ref.5
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 2 EF-hand domains.
Caution	It is unlikely that this protein binds calcium as none of the 2 EF-hand domains seem to contain a canonical calcium-binding site.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6MZZ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6MZZ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     417-423: RFREKFP → KHCPKLK
     424-669: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 669

669

Calpain-13

PRO_0000259582

Regions

Domain

34 – 333

300

Calpain catalytic

Domain

538 – 573

36

EF-hand 1

Domain

636 – 669

34

EF-hand 2

Sites

Active site

93

1

By similarity

Active site

249

1

By similarity

Active site

273

1

By similarity

Natural variations

Alternative sequence

417 – 423

7

RFREKFP → KHCPKLK in isoform 2.

VSP_021476

Alternative sequence

424 – 669

246

Missing in isoform 2.

VSP_021477

Natural variant

249

1

H → Y.
Corresponds to variant rs17010210 [ dbSNP | Ensembl ].

VAR_028964

Natural variant

280

1

A → T. Ref.1 Ref.4
Corresponds to variant rs508405 [ dbSNP | Ensembl ].

VAR_028965

Natural variant

596

1

I → T.
Corresponds to variant rs2276568 [ dbSNP | Ensembl ].

VAR_028966

Experimental info

Sequence conflict

562

1

E → G in CAE45878. Ref.2

Secondary structure

1

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669

Helix Strand Turn

Details...

Helix

518 – 529

12

Helix

540 – 550

11

Beta strand

555 – 558

4

Helix

560 – 580

21

Beta strand

586 – 588

3

Helix

589 – 591

3

Helix

592 – 597

6

Helix

600 – 602

3

Helix

609 – 619

11

Beta strand

624 – 626

3

Helix

628 – 648

21

Beta strand

650 – 653

4

Helix

659 – 667

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: BB24CF09D55CF790	FASTA	669	76,696
	10 20 30 40 50 60 MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK LLQEKRLSNV 70 80 90 100 110 120 IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG SLTQNPQYRQ KILMVQSFSH 130 140 150 160 170 180 QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY 190 200 210 220 230 240 SDLHYGFLED ALVDLTGGVI TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA 250 260 270 280 290 300 MENGLVSLHA YTVTGAEQIQ YRRGWEEIIS LWNPWGWGEA EWRGRWSDGS QEWEETCDPR 310 320 330 340 350 360 KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI MFRKQVILGN 370 380 390 400 410 420 TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE DAKFPLDFQV ILAGSQRFRE 430 440 450 460 470 480 KFPPVFFSSF RNTVQSSNNK FRRNFTMTYH LSPGNYVVVA QTRRKSAEFL LRIFLKMPDS 490 500 510 520 530 540 DRHLSSHFNL RMKGSPSEHG SQQSIFNRYA QQRLDIDATQ LQGLLNQELL TGPPGDMFSL 550 560 570 580 590 600 DECRSLVALM ELKVNGRLDQ EEFARLWKRL VHYQHVFQKV QTSPGVLLSS DLWKAIENTD 610 620 630 640 650 660 FLRGIFISRE LLHLVTLRYS DSVGRVSFPS LVCFLMRLEA MAKTFRNLSK DGKGLYLTEM EWMSLVMYN
	Isoform 2 [UniParc]. Checksum: 54122B6EFF505BCC	FASTA	423	48,002
	10 20 30 40 50 60 MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK LLQEKRLSNV 70 80 90 100 110 120 IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG SLTQNPQYRQ KILMVQSFSH 130 140 150 160 170 180 QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY 190 200 210 220 230 240 SDLHYGFLED ALVDLTGGVI TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA 250 260 270 280 290 300 MENGLVSLHA YTVTGAEQIQ YRRGWEEIIS LWNPWGWGEA EWRGRWSDGS QEWEETCDPR 310 320 330 340 350 360 KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI MFRKQVILGN 370 380 390 400 410 420 TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE DAKFPLDFQV ILAGSQKHCP KLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-280.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Esophageal carcinoma.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-280. Tissue: Colon.
[5]	"Identification and characterization of two novel calpain large subunit genes." Dear T.N., Boehm T. Gene 274:245-252(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Structure of human calpain 13." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 515-669.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK074418 mRNA. Translation: BAB85075.1.
BX640793 mRNA. Translation: CAE45878.1.
AC092569 Genomic DNA. Translation: AAX82015.1.
BC117347 mRNA. Translation: AAI17348.1.
BC117345 mRNA. Translation: AAI17346.1.

IPI

IPI00418334.
IPI00479445.

RefSeq

NP_653176.2. NM_144575.2.

UniGene

Hs.660911.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I7A	X-ray	1.80	A	515-669	[»]

ProteinModelPortal

Q6MZZ7.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000295055.

Protein family/group databases

MEROPS

C02.020.

PTM databases

PhosphoSite

Q6MZZ7.

Polymorphism databases

DMDM

117949375.

Proteomic databases

PaxDb

Q6MZZ7.

PRIDE

Q6MZZ7.

Protocols and materials databases

DNASU

92291.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000295055; ENSP00000295055; ENSG00000162949.
ENST00000458085; ENSP00000416191; ENSG00000162949.

GeneID

92291.

KEGG

hsa:92291.

UCSC

uc002rnp.1. human.

Organism-specific databases

CTD

92291.

GeneCards

GC02M030945.

H-InvDB

HIX0018641.

HGNC

HGNC:16663. CAPN13.

HPA

HPA030034.

MIM

610228. gene.

neXtProt

NX_Q6MZZ7.

PharmGKB

PA134931170.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG257157.

HOGENOM

HOG000232035.

HOVERGEN

HBG012645.

KO

K08581.

OMA

YLTEMEW.

Gene expression databases

ArrayExpress

Q6MZZ7.

Bgee

Q6MZZ7.

CleanEx

HS_CAPN13.

Genevestigator

Q6MZZ7.

GermOnline

ENSG00000162949. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR011992. EF-hand-like_dom.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]

Pfam

PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]

PRINTS

PR00704. CALPAIN.

SMART

SM00230. CysPc. 1 hit.
[Graphical view]

SUPFAM

SSF49758. Peptidase_C2. 1 hit.

PROSITE

PS50203. CALPAIN_CAT. 1 hit.
PS50222. EF_HAND_2. False negative.
PS00640. THIOL_PROTEASE_ASN. False negative.
PS00139. THIOL_PROTEASE_CYS. False negative.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CAPN13. human.

EvolutionaryTrace

Q6MZZ7.

GenomeRNAi

92291.

NextBio

77670.

SOURCE

Search...

Entry information

Entry name

CAN13_HUMAN

Accession

Primary (citable) accession number: Q6MZZ7
Secondary accession number(s): Q17RF0, Q580X1, Q8TE80

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.