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Q6MZZ7 (CAN13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calpain-13

EC=3.4.22.-
Alternative name(s):
Calcium-activated neutral proteinase 13
Short name=CANP 13
Gene names
Name:CAPN13
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable non-lysosomal thiol-protease By similarity.

Tissue specificity

Weakly expressed in lung and testis. Weakly or not expressed in other tissues. Ref.5

Sequence similarities

Belongs to the peptidase C2 family.

Contains 1 calpain catalytic domain.

Contains 2 EF-hand domains.

Caution

It is unlikely that this protein binds calcium as none of the 2 EF-hand domains seem to contain a canonical calcium-binding site.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   Molecular functionHydrolase
Protease
Thiol protease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

calcium-dependent cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6MZZ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6MZZ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     417-423: RFREKFP → KHCPKLK
     424-669: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 669669Calpain-13
PRO_0000259582

Regions

Domain34 – 333300Calpain catalytic
Domain538 – 57336EF-hand 1
Domain636 – 66934EF-hand 2

Sites

Active site931 By similarity
Active site2491 By similarity
Active site2731 By similarity

Natural variations

Alternative sequence417 – 4237RFREKFP → KHCPKLK in isoform 2.
VSP_021476
Alternative sequence424 – 669246Missing in isoform 2.
VSP_021477
Natural variant2491H → Y.
Corresponds to variant rs17010210 [ dbSNP | Ensembl ].
VAR_028964
Natural variant2801A → T. Ref.1 Ref.4
Corresponds to variant rs508405 [ dbSNP | Ensembl ].
VAR_028965
Natural variant5961I → T.
Corresponds to variant rs2276568 [ dbSNP | Ensembl ].
VAR_028966

Experimental info

Sequence conflict5621E → G in CAE45878. Ref.2

Secondary structure

......................... 669
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: BB24CF09D55CF790

FASTA66976,696
        10         20         30         40         50         60 
MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK LLQEKRLSNV 

        70         80         90        100        110        120 
IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG SLTQNPQYRQ KILMVQSFSH 

       130        140        150        160        170        180 
QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY 

       190        200        210        220        230        240 
SDLHYGFLED ALVDLTGGVI TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA 

       250        260        270        280        290        300 
MENGLVSLHA YTVTGAEQIQ YRRGWEEIIS LWNPWGWGEA EWRGRWSDGS QEWEETCDPR 

       310        320        330        340        350        360 
KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI MFRKQVILGN 

       370        380        390        400        410        420 
TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE DAKFPLDFQV ILAGSQRFRE 

       430        440        450        460        470        480 
KFPPVFFSSF RNTVQSSNNK FRRNFTMTYH LSPGNYVVVA QTRRKSAEFL LRIFLKMPDS 

       490        500        510        520        530        540 
DRHLSSHFNL RMKGSPSEHG SQQSIFNRYA QQRLDIDATQ LQGLLNQELL TGPPGDMFSL 

       550        560        570        580        590        600 
DECRSLVALM ELKVNGRLDQ EEFARLWKRL VHYQHVFQKV QTSPGVLLSS DLWKAIENTD 

       610        620        630        640        650        660 
FLRGIFISRE LLHLVTLRYS DSVGRVSFPS LVCFLMRLEA MAKTFRNLSK DGKGLYLTEM 


EWMSLVMYN 

« Hide

Isoform 2 [UniParc].

Checksum: 54122B6EFF505BCC
Show »

FASTA42348,002

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-280.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Esophageal carcinoma.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-280.
Tissue: Colon.
[5]"Identification and characterization of two novel calpain large subunit genes."
Dear T.N., Boehm T.
Gene 274:245-252(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Structure of human calpain 13."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 515-669.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK074418 mRNA. Translation: BAB85075.1.
BX640793 mRNA. Translation: CAE45878.1.
AC092569 Genomic DNA. Translation: AAX82015.1.
BC117347 mRNA. Translation: AAI17348.1.
BC117345 mRNA. Translation: AAI17346.1.
RefSeqNP_653176.2. NM_144575.2.
UniGeneHs.660911.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2I7AX-ray1.80A515-669[»]
ProteinModelPortalQ6MZZ7.
SMRQ6MZZ7. Positions 14-669.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000295055.

Protein family/group databases

MEROPSC02.020.

PTM databases

PhosphoSiteQ6MZZ7.

Polymorphism databases

DMDM117949375.

Proteomic databases

PaxDbQ6MZZ7.
PRIDEQ6MZZ7.

Protocols and materials databases

DNASU92291.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000295055; ENSP00000295055; ENSG00000162949. [Q6MZZ7-1]
ENST00000458085; ENSP00000416191; ENSG00000162949. [Q6MZZ7-2]
GeneID92291.
KEGGhsa:92291.
UCSCuc002rnp.1. human. [Q6MZZ7-1]

Organism-specific databases

CTD92291.
GeneCardsGC02M030945.
H-InvDBHIX0018641.
HGNCHGNC:16663. CAPN13.
HPAHPA029497.
HPA030034.
MIM610228. gene.
neXtProtNX_Q6MZZ7.
PharmGKBPA134931170.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG257157.
HOGENOMHOG000232035.
HOVERGENHBG012645.
KOK08581.
OMAYLTEMEW.
OrthoDBEOG7RV9FM.
PhylomeDBQ6MZZ7.
TreeFamTF314748.

Gene expression databases

ArrayExpressQ6MZZ7.
BgeeQ6MZZ7.
CleanExHS_CAPN13.
GenevestigatorQ6MZZ7.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR011992. EF-hand-dom_pair.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSPR00704. CALPAIN.
SMARTSM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMSSF49758. SSF49758. 1 hit.
PROSITEPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCAPN13. human.
EvolutionaryTraceQ6MZZ7.
GenomeRNAi92291.
NextBio77670.
PROQ6MZZ7.
SOURCESearch...

Entry information

Entry nameCAN13_HUMAN
AccessionPrimary (citable) accession number: Q6MZZ7
Secondary accession number(s): Q17RF0, Q580X1, Q8TE80
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM