Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein lin-54 homolog

Gene

LIN54

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-32117-MONOMER.
ReactomeiR-HSA-1538133. G0 and Early G1.
R-HSA-156711. Polo-like kinase mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-54 homolog
Alternative name(s):
CXC domain-containing protein 1
Gene namesi
Name:LIN54
Synonyms:CXCDC1, KIAA2037
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:25397. LIN54.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi525C → Y: Abolishes DNA-binding to the CDK1 promoter; when associated with Y-527. 1 Publication1
Mutagenesisi527C → Y: Abolishes DNA-binding to the CDK1 promoter; when associated with Y-525. 1 Publication1

Organism-specific databases

DisGeNETi132660.
OpenTargetsiENSG00000189308.
PharmGKBiPA162394056.

Polymorphism and mutation databases

DMDMi313104222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003413891 – 749Protein lin-54 homologAdd BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244N6-acetyllysineCombined sources1
Modified residuei249N6-acetyllysineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei282PhosphoserineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei635PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6MZP7.
MaxQBiQ6MZP7.
PaxDbiQ6MZP7.
PeptideAtlasiQ6MZP7.
PRIDEiQ6MZP7.

PTM databases

iPTMnetiQ6MZP7.
PhosphoSitePlusiQ6MZP7.

Expressioni

Gene expression databases

BgeeiENSG00000189308.
CleanExiHS_LIN54.
ExpressionAtlasiQ6MZP7. baseline and differential.
GenevisibleiQ6MZP7. HS.

Organism-specific databases

HPAiHPA056606.

Interactioni

Subunit structurei

Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYBL2P102444EBI-1389411,EBI-1389468
RBL2Q0899910EBI-1389411,EBI-971439

Protein-protein interaction databases

BioGridi126328. 25 interactors.
IntActiQ6MZP7. 41 interactors.
MINTiMINT-7239317.
STRINGi9606.ENSP00000341947.

Structurei

Secondary structure

1749
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi528 – 530Combined sources3
Helixi538 – 541Combined sources4
Helixi558 – 560Combined sources3
Helixi561 – 574Combined sources14
Turni576 – 579Combined sources4
Beta strandi602 – 604Combined sources3
Helixi612 – 615Combined sources4
Helixi636 – 642Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FD3X-ray2.42A/B515-646[»]
ProteinModelPortaliQ6MZP7.
SMRiQ6MZP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini521 – 634CRCPROSITE-ProRule annotationAdd BLAST114

Domaini

The CXC domains mediate DNA-binding.1 Publication

Sequence similaritiesi

Belongs to the lin-54 family.Curated
Contains 1 CRC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1171. Eukaryota.
ENOG4110UR5. LUCA.
GeneTreeiENSGT00390000013974.
HOGENOMiHOG000082518.
HOVERGENiHBG108088.
InParanoidiQ6MZP7.
OMAiTPGIQTQ.
OrthoDBiEOG091G09E2.
PhylomeDBiQ6MZP7.
TreeFamiTF313189.

Family and domain databases

InterProiIPR005172. CRC.
IPR028307. Lin-54_fam.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR12446. PTHR12446. 1 hit.
PfamiPF03638. TCR. 2 hits.
[Graphical view]
SMARTiSM01114. CXC. 2 hits.
[Graphical view]
PROSITEiPS51634. CRC. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6MZP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVVPAEVNS LLPEEIMDTG ITLVDDDSIE AVIVSSPIPM ETELEEIVNI
60 70 80 90 100
NSTGDSTATP ISTEPITVYS NHTNQVAVNT TITKADSNTT VKPAFPSGLQ
110 120 130 140 150
KLGAQTPVTI SANQIILNKV SQTSDLKLGN QTLKPDGQKL ILTTLGKSGS
160 170 180 190 200
PIVLALPHSQ LPQAQKVTTQ AQSGDAKLPP QQIKVVTIGG RPEVKPVIGV
210 220 230 240 250
SALTPGSQLI NTTTQPSVLQ TQQLKTVQIA KKPRTPTSGP VITKLIFAKP
260 270 280 290 300
INSKAVTGQT TQVSPPVIAG RVLSQSTPGT PSKTITISES GVIGSTLNST
310 320 330 340 350
TQTPNKIAIS PLKSPNKAVK STVQTITVGG VSTSQFKTII PLATAPNVQQ
360 370 380 390 400
IQVPGSKFHY VRLVTATSAS SSTQPVSQNP STNTQPLQQA KPVVVNTTPV
410 420 430 440 450
RMSVPIVSAQ AVKQVVPKPI NPTSQIVTTS QPQQRLIMPA TPLPQIQPNL
460 470 480 490 500
TNLPPGTVLA PAPGTGNVGY AVLPAQYVTQ LQQSSYVSIA SNSTFTGTSG
510 520 530 540 550
IQTQARLPFN GIIPSESASR PRKPCNCTKS LCLKLYCDCF ANGEFCNNCN
560 570 580 590 600
CTNCYNNLEH ENERQKAIKA CLDRNPEAFK PKIGKGKEGE SDRRHSKGCN
610 620 630 640 650
CKRSGCLKNY CECYEAKIMC SSICKCIGCK NFEESPERKT LMHLADAAEV
660 670 680 690 700
RVQQQTAAKT KLSSQISDLL TRPTPALNSG GGKLPFTFVT KEVAEATCNC
710 720 730 740
LLAQAEQADK KGKSKAAAER MILEEFGRCL MSVINSAGKA KSDPCAMNC
Length:749
Mass (Da):79,494
Last modified:November 30, 2010 - v3
Checksum:iA3E4513CB0118B78
GO
Isoform 2 (identifier: Q6MZP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-317: Missing.

Note: No experimental confirmation available.
Show »
Length:660
Mass (Da):70,378
Checksum:iF34917B51B87B370
GO
Isoform 3 (identifier: Q6MZP7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     8-228: Missing.

Show »
Length:528
Mass (Da):56,284
Checksum:i7EEAFDD538973450
GO
Isoform 4 (identifier: Q6MZP7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-401: Missing.

Note: No experimental confirmation available.
Show »
Length:348
Mass (Da):37,613
Checksum:iB65DCCA3258768FB
GO
Isoform 5 (identifier: Q6MZP7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-243: IAKKPRTPTSGPVIT → VGFFHSLLPELHQRP
     244-749: Missing.

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):25,743
Checksum:i6ECC642FE3E55419
GO

Sequence cautioni

The sequence BAC98377 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9N → S in CAE45981 (PubMed:17974005).Curated1
Sequence conflicti743D → A in CAE45981 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0342721 – 401Missing in isoform 4. 1 PublicationAdd BLAST401
Alternative sequenceiVSP_0342738 – 228Missing in isoform 3. 2 PublicationsAdd BLAST221
Alternative sequenceiVSP_034274229 – 317Missing in isoform 2. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_034275229 – 243IAKKP…GPVIT → VGFFHSLLPELHQRP in isoform 5. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_034276244 – 749Missing in isoform 5. 1 PublicationAdd BLAST506

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB111889 mRNA. Translation: BAC98377.1. Different initiation.
AK292769 mRNA. Translation: BAF85458.1.
BX640657 mRNA. Translation: CAE45799.1.
BX640966 mRNA. Translation: CAE45981.1.
AC021105 Genomic DNA. No translation available.
BC109277 mRNA. Translation: AAI09278.1.
BC109278 mRNA. Translation: AAI09279.1.
CCDSiCCDS3599.1. [Q6MZP7-1]
CCDS47089.1. [Q6MZP7-3]
CCDS75157.1. [Q6MZP7-2]
RefSeqiNP_001108479.1. NM_001115007.2. [Q6MZP7-3]
NP_001108480.1. NM_001115008.2. [Q6MZP7-3]
NP_001275925.1. NM_001288996.1. [Q6MZP7-2]
NP_001275926.1. NM_001288997.1. [Q6MZP7-3]
NP_919258.2. NM_194282.3. [Q6MZP7-1]
XP_005262807.1. XM_005262750.4. [Q6MZP7-1]
XP_006714144.1. XM_006714081.3. [Q6MZP7-1]
XP_016863217.1. XM_017007728.1. [Q6MZP7-2]
XP_016863218.1. XM_017007729.1. [Q6MZP7-4]
XP_016863219.1. XM_017007730.1. [Q6MZP7-4]
UniGeneiHs.96952.

Genome annotation databases

EnsembliENST00000340417; ENSP00000341947; ENSG00000189308. [Q6MZP7-1]
ENST00000442461; ENSP00000398265; ENSG00000189308. [Q6MZP7-3]
ENST00000446851; ENSP00000407139; ENSG00000189308. [Q6MZP7-3]
ENST00000505397; ENSP00000425844; ENSG00000189308. [Q6MZP7-1]
ENST00000506560; ENSP00000423475; ENSG00000189308. [Q6MZP7-2]
ENST00000508171; ENSP00000427413; ENSG00000189308. [Q6MZP7-5]
ENST00000510557; ENSP00000421045; ENSG00000189308. [Q6MZP7-3]
GeneIDi132660.
KEGGihsa:132660.
UCSCiuc003hnx.5. human. [Q6MZP7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB111889 mRNA. Translation: BAC98377.1. Different initiation.
AK292769 mRNA. Translation: BAF85458.1.
BX640657 mRNA. Translation: CAE45799.1.
BX640966 mRNA. Translation: CAE45981.1.
AC021105 Genomic DNA. No translation available.
BC109277 mRNA. Translation: AAI09278.1.
BC109278 mRNA. Translation: AAI09279.1.
CCDSiCCDS3599.1. [Q6MZP7-1]
CCDS47089.1. [Q6MZP7-3]
CCDS75157.1. [Q6MZP7-2]
RefSeqiNP_001108479.1. NM_001115007.2. [Q6MZP7-3]
NP_001108480.1. NM_001115008.2. [Q6MZP7-3]
NP_001275925.1. NM_001288996.1. [Q6MZP7-2]
NP_001275926.1. NM_001288997.1. [Q6MZP7-3]
NP_919258.2. NM_194282.3. [Q6MZP7-1]
XP_005262807.1. XM_005262750.4. [Q6MZP7-1]
XP_006714144.1. XM_006714081.3. [Q6MZP7-1]
XP_016863217.1. XM_017007728.1. [Q6MZP7-2]
XP_016863218.1. XM_017007729.1. [Q6MZP7-4]
XP_016863219.1. XM_017007730.1. [Q6MZP7-4]
UniGeneiHs.96952.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FD3X-ray2.42A/B515-646[»]
ProteinModelPortaliQ6MZP7.
SMRiQ6MZP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126328. 25 interactors.
IntActiQ6MZP7. 41 interactors.
MINTiMINT-7239317.
STRINGi9606.ENSP00000341947.

PTM databases

iPTMnetiQ6MZP7.
PhosphoSitePlusiQ6MZP7.

Polymorphism and mutation databases

DMDMi313104222.

Proteomic databases

EPDiQ6MZP7.
MaxQBiQ6MZP7.
PaxDbiQ6MZP7.
PeptideAtlasiQ6MZP7.
PRIDEiQ6MZP7.

Protocols and materials databases

DNASUi132660.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340417; ENSP00000341947; ENSG00000189308. [Q6MZP7-1]
ENST00000442461; ENSP00000398265; ENSG00000189308. [Q6MZP7-3]
ENST00000446851; ENSP00000407139; ENSG00000189308. [Q6MZP7-3]
ENST00000505397; ENSP00000425844; ENSG00000189308. [Q6MZP7-1]
ENST00000506560; ENSP00000423475; ENSG00000189308. [Q6MZP7-2]
ENST00000508171; ENSP00000427413; ENSG00000189308. [Q6MZP7-5]
ENST00000510557; ENSP00000421045; ENSG00000189308. [Q6MZP7-3]
GeneIDi132660.
KEGGihsa:132660.
UCSCiuc003hnx.5. human. [Q6MZP7-1]

Organism-specific databases

CTDi132660.
DisGeNETi132660.
GeneCardsiLIN54.
H-InvDBHIX0024607.
HGNCiHGNC:25397. LIN54.
HPAiHPA056606.
MIMi613367. gene.
neXtProtiNX_Q6MZP7.
OpenTargetsiENSG00000189308.
PharmGKBiPA162394056.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1171. Eukaryota.
ENOG4110UR5. LUCA.
GeneTreeiENSGT00390000013974.
HOGENOMiHOG000082518.
HOVERGENiHBG108088.
InParanoidiQ6MZP7.
OMAiTPGIQTQ.
OrthoDBiEOG091G09E2.
PhylomeDBiQ6MZP7.
TreeFamiTF313189.

Enzyme and pathway databases

BioCyciZFISH:G66-32117-MONOMER.
ReactomeiR-HSA-1538133. G0 and Early G1.
R-HSA-156711. Polo-like kinase mediated events.

Miscellaneous databases

ChiTaRSiLIN54. human.
GenomeRNAii132660.
PROiQ6MZP7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000189308.
CleanExiHS_LIN54.
ExpressionAtlasiQ6MZP7. baseline and differential.
GenevisibleiQ6MZP7. HS.

Family and domain databases

InterProiIPR005172. CRC.
IPR028307. Lin-54_fam.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR12446. PTHR12446. 1 hit.
PfamiPF03638. TCR. 2 hits.
[Graphical view]
SMARTiSM01114. CXC. 2 hits.
[Graphical view]
PROSITEiPS51634. CRC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIN54_HUMAN
AccessioniPrimary (citable) accession number: Q6MZP7
Secondary accession number(s): Q32M68
, Q32M69, Q6N071, Q76B60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.