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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycoplasma mycoides subsp. mycoides SC (strain PG1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MSC_0131
OrganismiMycoplasma mycoides subsp. mycoides SC (strain PG1)
Taxonomic identifieri272632 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001016 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196051 – 483Glutamate--tRNA ligaseAdd BLAST483

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272632.MSC_0131.

Structurei

3D structure databases

ProteinModelPortaliQ6MUA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionAdd BLAST11
Motifi253 – 257"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiLKTVEWT.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MUA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRLRYAPS PTGFLHIGNT RTALMNYLFA KHYNGSFIVR IEDTDLARNV
60 70 80 90 100
DGAIESQFEN LNWLGIFPDE SIFNVGDQKY GKYMQSQKFD RYKQLAEQLV
110 120 130 140 150
NQNKAYRCFC SSEELEKDYE EQTSKEIIAT KYSQKCLFLT QDQISQNLKD
160 170 180 190 200
KKEYSIRFKV PQNKVWTIND IVRGDVSFDS KDLGDFVILK SNGVATYNFA
210 220 230 240 250
VVIDDYDMQI THVLRGEEHI SNTPRQMMIY DAFNWNYPKF GHLTLIVDNT
260 270 280 290 300
GKKLSKRSGN ALFFIEQYKK QGYLSQAIFN YIALLGWSPP GEQEILSQNE
310 320 330 340 350
LIKIFDEKRF SKSPSTFDMV KMKWINSVYM KKLEDNEYLE FVKSFINTNK
360 370 380 390 400
FDITSKSEAW LNHLLLLYKK ELEYAEQIND HLDLFFNKNT LDNNTIDVLN
410 420 430 440 450
NLTNYKNVVE IFKNQINDLK DWTIENIKQI IKDTSTLANV KGKDLFMPIR
460 470 480
IFATKSEHGP SLADVIYLLG KTTVLNNINS LER
Length:483
Mass (Da):56,387
Last modified:July 5, 2004 - v1
Checksum:iF6F4E94038375B34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX293980 Genomic DNA. Translation: CAE76778.1.
RefSeqiNP_975136.1. NC_005364.2.
WP_011166335.1. NC_005364.2.

Genome annotation databases

EnsemblBacteriaiCAE76778; CAE76778; MSC_0131.
GeneIDi2745203.
KEGGimmy:MSC_0131.
PATRICi20016966. VBIMycMyc4973_0139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX293980 Genomic DNA. Translation: CAE76778.1.
RefSeqiNP_975136.1. NC_005364.2.
WP_011166335.1. NC_005364.2.

3D structure databases

ProteinModelPortaliQ6MUA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272632.MSC_0131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE76778; CAE76778; MSC_0131.
GeneIDi2745203.
KEGGimmy:MSC_0131.
PATRICi20016966. VBIMycMyc4973_0139.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiLKTVEWT.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_MYCMS
AccessioniPrimary (citable) accession number: Q6MUA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.