Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6MUA6 (SYE_MYCMS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MSC_0131
OrganismMycoplasma mycoides subsp. mycoides SC (strain PG1) [Reference proteome] [HAMAP]
Taxonomic identifier272632 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119605

Regions

Motif9 – 1911"HIGH" region HAMAP-Rule MF_00022
Motif253 – 2575"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2561ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MUA6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: F6F4E94038375B34

FASTA48356,387
        10         20         30         40         50         60 
MSFRLRYAPS PTGFLHIGNT RTALMNYLFA KHYNGSFIVR IEDTDLARNV DGAIESQFEN 

        70         80         90        100        110        120 
LNWLGIFPDE SIFNVGDQKY GKYMQSQKFD RYKQLAEQLV NQNKAYRCFC SSEELEKDYE 

       130        140        150        160        170        180 
EQTSKEIIAT KYSQKCLFLT QDQISQNLKD KKEYSIRFKV PQNKVWTIND IVRGDVSFDS 

       190        200        210        220        230        240 
KDLGDFVILK SNGVATYNFA VVIDDYDMQI THVLRGEEHI SNTPRQMMIY DAFNWNYPKF 

       250        260        270        280        290        300 
GHLTLIVDNT GKKLSKRSGN ALFFIEQYKK QGYLSQAIFN YIALLGWSPP GEQEILSQNE 

       310        320        330        340        350        360 
LIKIFDEKRF SKSPSTFDMV KMKWINSVYM KKLEDNEYLE FVKSFINTNK FDITSKSEAW 

       370        380        390        400        410        420 
LNHLLLLYKK ELEYAEQIND HLDLFFNKNT LDNNTIDVLN NLTNYKNVVE IFKNQINDLK 

       430        440        450        460        470        480 
DWTIENIKQI IKDTSTLANV KGKDLFMPIR IFATKSEHGP SLADVIYLLG KTTVLNNINS 


LER 

« Hide

References

[1]"The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)."
Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M.
Genome Res. 14:221-227(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PG1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX293980 Genomic DNA. Translation: CAE76778.1.
RefSeqNP_975136.1. NC_005364.2.

3D structure databases

ProteinModelPortalQ6MUA6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272632.MSC_0131.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE76778; CAE76778; MSC_0131.
GeneID2745203.
KEGGmmy:MSC_0131.
PATRIC20016966. VBIMycMyc4973_0139.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAAFRCFCT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycMMYC272632:GI1G-131-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYCMS
AccessionPrimary (citable) accession number: Q6MUA6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries