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Q6MU75 (TRMB_MYCMS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MSC_0166
OrganismMycoplasma mycoides subsp. mycoides SC (strain PG1) [Reference proteome] [HAMAP]
Taxonomic identifier272632 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 221221tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171356

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1231 By similarity
Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site1231S-adenosyl-L-methionine By similarity
Binding site1271Substrate By similarity
Binding site1591Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MU75 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: D5F997239233E2D1

FASTA22126,250
        10         20         30         40         50         60 
MRLRKKNWTD DFLNQHSFYL INYNNKKIDL KQIFLNNNPT CLEIGSGKGQ FITTLALKNL 

        70         80         90        100        110        120 
NTNYIGMEKS STITGVALKK SLKEFENQLK DMTNLKYFNN FAEDLSQMFS SDSFNKIYLN 

       130        140        150        160        170        180 
FSDPWPKTRH YKKRLTYVKF LDIYSDILIK NGYLEFKTDN DSLYNFTIEQ LNLTNKWEIV 

       190        200        210        220 
INATDLYNNT EFLKDNIPTE YETKFHLANK NIYKIVIKNL K 

« Hide

References

[1]"The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)."
Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M.
Genome Res. 14:221-227(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PG1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX293980 Genomic DNA. Translation: CAE76811.1.
RefSeqNP_975169.1. NC_005364.2.

3D structure databases

ProteinModelPortalQ6MU75.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272632.MSC_0166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE76811; CAE76811; MSC_0166.
GeneID2744317.
KEGGmmy:MSC_0166.
PATRIC20017040. VBIMycMyc4973_0176.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKGRFLMD.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK745688.

Enzyme and pathway databases

BioCycMMYC272632:GI1G-164-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCMS
AccessionPrimary (citable) accession number: Q6MU75
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways