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Q6MT28

- SYI_MYCMS

UniProt

Q6MT28 - SYI_MYCMS

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma mycoides subsp. mycoides SC (strain PG1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi883 – 8831ZincUniRule annotation
Metal bindingi886 – 8861ZincUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMYC272632:GI1G-570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MSC_0585
OrganismiMycoplasma mycoides subsp. mycoides SC (strain PG1)
Taxonomic identifieri272632 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001016: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Isoleucine--tRNA ligasePRO_0000098422Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272632.MSC_0585.

Structurei

3D structure databases

ProteinModelPortaliQ6MT28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MT28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNKYKDTLL IGKTDFDMRA NLNQKEPKFE QFWQENQIYN KKLKLNENKK
60 70 80 90 100
VFVLHDGPPY ANGDLHIGHA LNKTLKDFII RFKNTTGYYA PFIMGWDTHG
110 120 130 140 150
LPIESAVTKM GVDRKSMSSV AFRELCYKYA LEQVSNQANQ FNRLGMFTDY
160 170 180 190 200
DTKYVTLTHD FEMSELRLFE KMYQKGLIYK DLKPIYWSPS SESALADSEI
210 220 230 240 250
IYKDISSPSI YVGCDVINSD EFKNTQLIIW TTTPWTLPSN QLIAIGSKIK
260 270 280 290 300
YSLIQVENSD KQFILASDLI NQVSQNIGWE NVKVIKEIDA NKLVGLNYVH
310 320 330 340 350
PLYDTKISKV VLGHHVTSES GSGLVHIASG FGEDDFLIAK QNNIKPFAPI
360 370 380 390 400
DNQGKFTTQI SDLDPQLVGM FYDDTNKIIT KRLEENKKLL KLKFLTHSYP
410 420 430 440 450
HDWRTKKPVI YRCTLQWFVN LAPVKNDILK NVDQINTHPK WAKKRLYQVL
460 470 480 490 500
DERTDWTISR QRLWGVPIIA FYDQNDDLVL NEQILNYAIN KIEEVGTNAW
510 520 530 540 550
FSLDADEFLP EQYKNKSLKK EKDILDVWFD SGSSAIALSQ KYPNLKLPYD
560 570 580 590 600
MYLEGNDQYR GWFNASMINS TIYSNTSPYK QLVSHGMTTD EKGNKMSKSL
610 620 630 640 650
GNGVDPIAFA NDLGADILRL WVASTDFTDD QKIGKEIIKQ ISESYRKIRN
660 670 680 690 700
TIRFILANLN DFNVKTDYQT KLSEVDMYSL FNLTSFKNKV IQAYQELNFS
710 720 730 740 750
LVYTLVMNYV TKNLSAFYLD FIKDILYINS KNDLRRRQVQ TVLYEQLYCL
760 770 780 790 800
IDVLRPILVH TIEEVYQNLN DNNKVESVHL LDNKEQNFVY DKEFINKWDQ
810 820 830 840 850
VMILRDDVNK ALEISRENKI INKGFEAVVN IKLDKEYDDL KNIKDLSQIF
860 870 880 890 900
IVNSINFVDN IDNSFIKTNI SSIKVEQKQG LKCQRCWQIF DNLIDDEICN
910
HCNNVVKSLL ETKCE
Length:915
Mass (Da):106,344
Last modified:July 5, 2004 - v1
Checksum:i55288E92EC9CE1BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX293980 Genomic DNA. Translation: CAE77208.1.
RefSeqiNP_975566.1. NC_005364.2.
WP_011166763.1. NC_005364.2.

Genome annotation databases

EnsemblBacteriaiCAE77208; CAE77208; MSC_0585.
GeneIDi2745219.
KEGGimmy:MSC_0585.
PATRICi20017969. VBIMycMyc4973_0630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX293980 Genomic DNA. Translation: CAE77208.1 .
RefSeqi NP_975566.1. NC_005364.2.
WP_011166763.1. NC_005364.2.

3D structure databases

ProteinModelPortali Q6MT28.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272632.MSC_0585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE77208 ; CAE77208 ; MSC_0585 .
GeneIDi 2745219.
KEGGi mmy:MSC_0585.
PATRICi 20017969. VBIMycMyc4973_0630.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MMYC272632:GI1G-570-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)."
    Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M.
    Genome Res. 14:221-227(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PG1.

Entry informationi

Entry nameiSYI_MYCMS
AccessioniPrimary (citable) accession number: Q6MT28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3