Q6MT00 (NTPA_MYCMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

MSC_0616

Organism

Mycoplasma mycoides subsp. mycoides SC (strain PG1)

Taxonomic identifier

272632 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178194
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	41	1	Magnesium or manganese By similarity
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MT00 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EADD20A8FF455E50	FASTA	200	23,262
	10 20 30 40 50 60 MDKKVIYLAT TNKNKVKEFS EILKDYQIKS LLDIPEYVEI EENKKTFKQN ALLKAKHLAK 70 80 90 100 110 120 YINGVAIGDD TGICVKALND FPGIYSKRWA YPLTNHYDIC NKLLDKLKHI NQLNKRKAYM 130 140 150 160 170 180 TTAIALYDAI NKKQFVYQAR VNGYIDFQVN ESEFGFGYDF IFIPKGYDKA YSLMNSELKN 190 200 QISARKKAID KLIEYIDNVK

References

[1]

"The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)." Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M. Genome Res. 14:221-227(2004) [PubMed: 14762060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PG1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX293980 Genomic DNA. Translation: CAE77238.1.
RefSeq	NP_975596.1. NC_005364.2.
3D structure databases
ProteinModelPortal	Q6MT00.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2744394.
GenomeReviews	Gene locus MSC_0616 in contig BX293980_GR.
KEGG	mmy:MSC_0616.
PATRIC	20018041. VBIMycMyc4973_0663.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	YSKRYDQ.
PhylomeDB	Q6MT00.
ProtClustDB	CLSK745853.
Enzyme and pathway databases
BioCyc	MMYC272632:MSC_0616-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_MYCMS

Accession

Primary (citable) accession number: Q6MT00

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program