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Q6MSR0 (SYL_MYCMS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MSC_0709
OrganismMycoplasma mycoides subsp. mycoides SC (strain PG1) [Reference proteome] [HAMAP]
Taxonomic identifier272632 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence CAE77328.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152047

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MSR0 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 8BD0473253647145

FASTA80493,178
        10         20         30         40         50         60 
MDFSHKAIEK KWQKYWKENN IYKTTNNSEK KAYILDMFPY PSGAGLHVGH IKGYTATDVY 

        70         80         90        100        110        120 
SRFKRMQGYD VLHPIGWDAF GLPAEQYALK TGNDPREFTL QNIENFKIQL NKMGFSYDYD 

       130        140        150        160        170        180 
KEINTADPNY YKTTQWIFKQ LYKKGLAENR DIDVNWCQEL GTVLANDEII EKDGLMVSER 

       190        200        210        220        230        240 
GEYPVVKKKM RQWVLKITDY ADKLLDGLDN LDWPNSVKEL QRNWIGKSEG CEINFKSNDI 

       250        260        270        280        290        300 
NIPVFTTRAD TIFGVTYIVL ASENELVLKL TAPEKLEEVK KYIELTANKS EIERKDESRT 

       310        320        330        340        350        360 
KTGVFIGSYA TNPLTKEQIQ IWISDYVLND YGSGAIMAVP AHDKRDWDFA AKFNLPIKFV 

       370        380        390        400        410        420 
IQTKDQSKAF VGEGIHINSE FLNDLDRIQA LQVIHDYVDK NNLGKRKINY KLRDWLFSRQ 

       430        440        450        460        470        480 
RFYGEPFPVL YDKDNNIVLI EDNNLPITLP ITDYIKPTNT GESPLANVKN WVNVKIGDKE 

       490        500        510        520        530        540 
YKRETNTMPQ SAASSWYFIA YILANSKNNL IDLTSDEAKK RLEKWLPVDL YIGGQEHAVG 

       550        560        570        580        590        600 
HLLYARFWTH FLYDLGLLPT NEPFKRLFNQ GMILGPDNRK MSKSWGNVIN PDDVIDTHGA 

       610        620        630        640        650        660 
DALRLYEMFM GPLDASLPWS FDGLDASLKW LNRCYRMINK IEFSNTNNHK LDYVYNDVVK 

       670        680        690        700        710        720 
KVTQMIQELK FNTAISQLMV LVNAIYKEEL NTVYKPYIEG FVKMLSLFAP HLSEELWEKL 

       730        740        750        760        770        780 
GNNSSVTLQT WPEFDETKII KNAVVIALQV NGKLRSTIEV EKGTDKETLI KLAQENENII 

       790        800 
RFIKDHKNLK YIAVVDRIVN IVIE 

« Hide

References

[1]"The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)."
Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M.
Genome Res. 14:221-227(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PG1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX293980 Genomic DNA. Translation: CAE77328.1. Different initiation.
RefSeqNP_975686.1. NC_005364.2.

3D structure databases

ProteinModelPortalQ6MSR0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272632.MSC_0709.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE77328; CAE77328; MSC_0709.
GeneID2744315.
KEGGmmy:MSC_0709.
PATRIC20018249. VBIMycMyc4973_0763.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMMYC272632:GI1G-696-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCMS
AccessionPrimary (citable) accession number: Q6MSR0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries