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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma mycoides subsp. mycoides SC (strain PG1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMYC272632:GI1G-696-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MSC_0709
OrganismiMycoplasma mycoides subsp. mycoides SC (strain PG1)
Taxonomic identifieri272632 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001016: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_0000152047Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272632.MSC_0709.

Structurei

3D structure databases

ProteinModelPortaliQ6MSR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi580 – 5845"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MSR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSHKAIEK KWQKYWKENN IYKTTNNSEK KAYILDMFPY PSGAGLHVGH
60 70 80 90 100
IKGYTATDVY SRFKRMQGYD VLHPIGWDAF GLPAEQYALK TGNDPREFTL
110 120 130 140 150
QNIENFKIQL NKMGFSYDYD KEINTADPNY YKTTQWIFKQ LYKKGLAENR
160 170 180 190 200
DIDVNWCQEL GTVLANDEII EKDGLMVSER GEYPVVKKKM RQWVLKITDY
210 220 230 240 250
ADKLLDGLDN LDWPNSVKEL QRNWIGKSEG CEINFKSNDI NIPVFTTRAD
260 270 280 290 300
TIFGVTYIVL ASENELVLKL TAPEKLEEVK KYIELTANKS EIERKDESRT
310 320 330 340 350
KTGVFIGSYA TNPLTKEQIQ IWISDYVLND YGSGAIMAVP AHDKRDWDFA
360 370 380 390 400
AKFNLPIKFV IQTKDQSKAF VGEGIHINSE FLNDLDRIQA LQVIHDYVDK
410 420 430 440 450
NNLGKRKINY KLRDWLFSRQ RFYGEPFPVL YDKDNNIVLI EDNNLPITLP
460 470 480 490 500
ITDYIKPTNT GESPLANVKN WVNVKIGDKE YKRETNTMPQ SAASSWYFIA
510 520 530 540 550
YILANSKNNL IDLTSDEAKK RLEKWLPVDL YIGGQEHAVG HLLYARFWTH
560 570 580 590 600
FLYDLGLLPT NEPFKRLFNQ GMILGPDNRK MSKSWGNVIN PDDVIDTHGA
610 620 630 640 650
DALRLYEMFM GPLDASLPWS FDGLDASLKW LNRCYRMINK IEFSNTNNHK
660 670 680 690 700
LDYVYNDVVK KVTQMIQELK FNTAISQLMV LVNAIYKEEL NTVYKPYIEG
710 720 730 740 750
FVKMLSLFAP HLSEELWEKL GNNSSVTLQT WPEFDETKII KNAVVIALQV
760 770 780 790 800
NGKLRSTIEV EKGTDKETLI KLAQENENII RFIKDHKNLK YIAVVDRIVN

IVIE
Length:804
Mass (Da):93,178
Last modified:March 1, 2005 - v2
Checksum:i8BD0473253647145
GO

Sequence cautioni

The sequence CAE77328.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX293980 Genomic DNA. Translation: CAE77328.1. Different initiation.
RefSeqiNP_975686.1. NC_005364.2.

Genome annotation databases

EnsemblBacteriaiCAE77328; CAE77328; MSC_0709.
GeneIDi2744315.
KEGGimmy:MSC_0709.
PATRICi20018249. VBIMycMyc4973_0763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX293980 Genomic DNA. Translation: CAE77328.1. Different initiation.
RefSeqiNP_975686.1. NC_005364.2.

3D structure databases

ProteinModelPortaliQ6MSR0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272632.MSC_0709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE77328; CAE77328; MSC_0709.
GeneIDi2744315.
KEGGimmy:MSC_0709.
PATRICi20018249. VBIMycMyc4973_0763.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMMYC272632:GI1G-696-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)."
    Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.-E., Pettersson B., Uhlen M.
    Genome Res. 14:221-227(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PG1.

Entry informationi

Entry nameiSYL_MYCMS
AccessioniPrimary (citable) accession number: Q6MSR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: January 7, 2015
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.