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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Bd0365
OrganismiBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Taxonomic identifieri264462 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio
ProteomesiUP000008080: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 796796Leucine--tRNA ligasePRO_0000151977Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264462.Bd0365.

Structurei

3D structure databases

ProteinModelPortaliQ6MQU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi569 – 5735"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHVKGYLA.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6MQU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLNFTEYEA KWQKKWADAK AFQAETTSSK PKYYALDMFP YPSASGLHVG
60 70 80 90 100
HMASYTPGDI ISRYKRVNGF NVLHPMGYDA FGLPAEQYAI QTGVHPAITT
110 120 130 140 150
KKAIDSFRKT LQTFGFSFDW SREISTCEPD YYKWTQFIFL KLYERGLAYQ
160 170 180 190 200
KEVPVNWCPA LKTVLANDEV VDGKSERGGH PVIRVPMKQW MLKITDYAER
210 220 230 240 250
LLNDLDKLDW PERTKEGQRN WIGKSEGARI TFKVHGEKDT FEVFTTRPDT
260 270 280 290 300
LFGVTFMVMA PEHPLVKKIT SQPQYTAVEN YIADTAKKSE VDRKASTEKT
310 320 330 340 350
GVFTGAHATH PITGDKIEIW ISDYVLMDYG TGAIMAVPGH DARDFEFATK
360 370 380 390 400
FNIPIKSVLE SDMLPFEGDS IMINSEFLDG LNKTEAISKM LKHLEENKLG
410 420 430 440 450
VREVQYKLRD WLFSRQRYWG EPFPIVHFAD GSKGVPVNEL PVILPEVADY
460 470 480 490 500
EPADTGEAPL ARNADWVKYM DGDKEGRRET DTMPGAAGSS WYFLRYIDPK
510 520 530 540 550
NTEAPFSPEA EKYWMPVDLY VGGPEHTVGH LLYARFWTKV LFDCGLVTHD
560 570 580 590 600
EPFQKLAHQG MILGPDGEKM SKSRGNVISP EDIARSHGAD ALRTFISFMG
610 620 630 640 650
PVDKDKPWAP TGIDGVKRFL DRITRLVVND DGQLVATSEA LTPEIEKLVH
660 670 680 690 700
KTIKKVTEDI ESMSFNTAIS AMMILVNELY RAECRSVLAV KPLVQILAPF
710 720 730 740 750
APHLAEELWE KMNGEGLCAL APWPKYDNTL CADDTVTIGV QVNGKMRGTI
760 770 780 790
EIGVAASEQE AVAAAKAVQQ VAAVLGDKNP DKVIYKAGKI LNLIVK
Length:796
Mass (Da):89,437
Last modified:July 5, 2004 - v1
Checksum:i01B10754DBB8D8A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842646 Genomic DNA. Translation: CAE78015.1.
RefSeqiNP_967361.1. NC_005363.1.

Genome annotation databases

EnsemblBacteriaiCAE78015; CAE78015; Bd0365.
GeneIDi2734277.
KEGGibba:Bd0365.
PATRICi21075494. VBIBdeBac73187_0336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842646 Genomic DNA. Translation: CAE78015.1.
RefSeqiNP_967361.1. NC_005363.1.

3D structure databases

ProteinModelPortaliQ6MQU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264462.Bd0365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE78015; CAE78015; Bd0365.
GeneIDi2734277.
KEGGibba:Bd0365.
PATRICi21075494. VBIBdeBac73187_0336.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHVKGYLA.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
    Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
    Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Entry informationi

Entry nameiSYL_BDEBA
AccessioniPrimary (citable) accession number: Q6MQU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.