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Q6MQU3 (SYL_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bd0365
OrganismBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]
Taxonomic identifier264462 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151977

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif569 – 5735"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MQU3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 01B10754DBB8D8A5

FASTA79689,437
        10         20         30         40         50         60 
MSLNFTEYEA KWQKKWADAK AFQAETTSSK PKYYALDMFP YPSASGLHVG HMASYTPGDI 

        70         80         90        100        110        120 
ISRYKRVNGF NVLHPMGYDA FGLPAEQYAI QTGVHPAITT KKAIDSFRKT LQTFGFSFDW 

       130        140        150        160        170        180 
SREISTCEPD YYKWTQFIFL KLYERGLAYQ KEVPVNWCPA LKTVLANDEV VDGKSERGGH 

       190        200        210        220        230        240 
PVIRVPMKQW MLKITDYAER LLNDLDKLDW PERTKEGQRN WIGKSEGARI TFKVHGEKDT 

       250        260        270        280        290        300 
FEVFTTRPDT LFGVTFMVMA PEHPLVKKIT SQPQYTAVEN YIADTAKKSE VDRKASTEKT 

       310        320        330        340        350        360 
GVFTGAHATH PITGDKIEIW ISDYVLMDYG TGAIMAVPGH DARDFEFATK FNIPIKSVLE 

       370        380        390        400        410        420 
SDMLPFEGDS IMINSEFLDG LNKTEAISKM LKHLEENKLG VREVQYKLRD WLFSRQRYWG 

       430        440        450        460        470        480 
EPFPIVHFAD GSKGVPVNEL PVILPEVADY EPADTGEAPL ARNADWVKYM DGDKEGRRET 

       490        500        510        520        530        540 
DTMPGAAGSS WYFLRYIDPK NTEAPFSPEA EKYWMPVDLY VGGPEHTVGH LLYARFWTKV 

       550        560        570        580        590        600 
LFDCGLVTHD EPFQKLAHQG MILGPDGEKM SKSRGNVISP EDIARSHGAD ALRTFISFMG 

       610        620        630        640        650        660 
PVDKDKPWAP TGIDGVKRFL DRITRLVVND DGQLVATSEA LTPEIEKLVH KTIKKVTEDI 

       670        680        690        700        710        720 
ESMSFNTAIS AMMILVNELY RAECRSVLAV KPLVQILAPF APHLAEELWE KMNGEGLCAL 

       730        740        750        760        770        780 
APWPKYDNTL CADDTVTIGV QVNGKMRGTI EIGVAASEQE AVAAAKAVQQ VAAVLGDKNP 

       790 
DKVIYKAGKI LNLIVK 

« Hide

References

[1]"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX842646 Genomic DNA. Translation: CAE78015.1.
RefSeqNP_967361.1. NC_005363.1.

3D structure databases

ProteinModelPortalQ6MQU3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264462.Bd0365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE78015; CAE78015; Bd0365.
GeneID2734277.
KEGGbba:Bd0365.
PATRIC21075494. VBIBdeBac73187_0336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_BDEBA
AccessionPrimary (citable) accession number: Q6MQU3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries