Reviewed, UniProtKB/Swiss-Prot Q6MQS6 (DNAE2_BDEBA)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Bd0384

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	1061 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1061	1061	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103366

Sequences

	Sequence		Length	Mass (Da)
	Q6MQS6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C0C592AE67AB3C83	FASTA	1,061	121,567
	10 20 30 40 50 60 MTNKALIPSQ NSRLPAKPRP RAKGFVELLG RSNFSFLQGA SSPEEMVSQA VEFGYDGMAL 70 80 90 100 110 120 CDLNGLYGVV RGFQTIQSPS LFTASPQVKE NFHYLIGSEL TLTDESSVVL IPMNKQGYSH 130 140 150 160 170 180 LCEILTLGKR QAAKGFSKLS LEQIEKYNQG LLCLALPPWT DERYEKLEKI FDNRLYLPLW 190 200 210 220 230 240 RDLTWESQEF CRQGFALEEK YKAQLFVTQR PFMHSPERKP LFDVLTCTLH HTTLAEAKNK 250 260 270 280 290 300 LIQNSERCLK SLEDISWLWQ DRLDLVEKTV EISARVTFSL NEIRYRYPAS SLPSGMTPTE 310 320 330 340 350 360 YMRHLTLKGA ESRYPEGIPD KALKQIEHEL ELIKDLQYED YFLTLKEICD FASERKILYQ 370 380 390 400 410 420 GRGSAANSVV CFCLGLTAID PVKMDLLFER FLSRERREPP DIDIDFEHSR REEVIQHIYE 430 440 450 460 470 480 KYNERHAAMV CTVIRYRSRM AIRETAKVFG MPLAKINAMI KFMGRDGMKR LLDPAVAADF 490 500 510 520 530 540 GMEPGPWKMF MALAQQLHGF PRHLGIHTGG FLITQDPITE MVPVEKATMN GRYVIQWNKD 550 560 570 580 590 600 DVDFLRLMKI DVLSLGMLTC LRKCFDLLRD VKGRDYSLAT LPADDKPTYD MICRAETVGV 610 620 630 640 650 660 FQIESRAQMN TLPRMQPRNF YDLVIEIALI RPGPLQGGMV HPFLKLRQNP PKEIKYAHPA 670 680 690 700 710 720 LEPILKKTMG IPIFQEQVMK MVVAVADFTP GEADELRRVM SSAWKRKATM GGIEQRLRTG 730 740 750 760 770 780 FARHGISSEY ADQIFKTIEG FANYGFPESH SASFALLTYA SCYLKCHYPD VFACGLLNSQ 790 800 810 820 830 840 PMGFYAPRTI IAEAQRNGVV VAPLDVQKSD YDYTIEKTAA GDLLRVGLRS IYGIPEVLIR 850 860 870 880 890 900 RIEDSRKEEG LYIDLADFIR RTQLPRSVLL KLAAAGAFES FNSNVRELIW NLESLSLDQQ 910 920 930 940 950 960 SFLWGHPKEQ FELGEDDDEP EHLPFESNWD RLRREYDSKG YSVESHPMSV LRTYLHSKSE 970 980 990 1000 1010 1020 ALREKRFVPY FSSEDLKRMK TKTKVRVAGL VAITQRPPTA KGMCFITLED EFGFVNIVIH 1030 1040 1050 1060 PEIYQKDRTT IYTRSLLEIH GQVEKVGAIT NIRAERILPL G

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842647 Genomic DNA. Translation: CAE78371.1.
RefSeq	NP_967378.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2733452.
GenomeReviews	Gene locus Bd0384 in contig BX842601_GR.
KEGG	bba:Bd0384.
NMPDR	fig|264462.1.peg.348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MQS6.
Enzyme and pathway databases
BioCyc	BBAC264462:BD0384-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BDEBA

Accession

Primary (citable) accession number: Q6MQS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)