Q6MNQ2 (SYY_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	Bd1189

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Tyrosine--tRNA ligase HAMAP MF_02007		PRO_0000236695
Regions
	Domain	341 – 404	64	S4 RNA-binding
	Motif	46 – 55	10	"HIGH" region HAMAP MF_02007
	Motif	230 – 234	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	233	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MNQ2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 00B1E7BC51690858	FASTA	405	45,368
	10 20 30 40 50 60 MMSPQEQLER IKFGTADFIN DEDMLKKLKR SIETKKPLNI KLGADPTRPD IHLGHTVVIN 70 80 90 100 110 120 KLKTFQDLGH KVSFLIGDFT AMIGDPSGKN STRPMLTREE IEENGRSYAK QIFKILDPEK 130 140 150 160 170 180 TEIVYNSSWI MKMTPAEFIT MTSKYTVAQL LEREDFTKRY RSGTPIGIHE FIYPLTQGYD 190 200 210 220 230 240 SVALKTDVEL GGTDQKFNLL VGRAMQAAYG MEAQCVLTMP ILEGIDGVNK MSKSLDNYIS 250 260 270 280 290 300 VVDTPKDMFG KTMRISDELM YRWYELLTDV GAAGLNQLRA DVAEGRKHPR TVKVELAKFL 310 320 330 340 350 360 IKRFHSQAEA QAAEDEFNRI FVEKGLPDEV PDFEVEAETQ MGLAALMVKA QLAASNSEAG 370 380 390 400 RLIQGGGVQI DGEKVSDPRL KIDLKSGASF VLKAGKKKFV KIVVK

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842649 Genomic DNA. Translation: CAE79099.1.
RefSeq	NP_968106.1. NC_005363.1.
3D structure databases
ProteinModelPortal	Q6MNQ2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2733227.
GenomeReviews	Gene locus Bd1189 in contig BX842601_GR.
KEGG	bba:Bd1189.
NMPDR	fig|264462.1.peg.1076.
PATRIC	21076990. VBIBdeBac73187_1076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288125.
OMA	YVVQVGK.
PhylomeDB	Q6MNQ2.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1189-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BDEBA

Accession

Primary (citable) accession number: Q6MNQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program