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Q6MNP6 (NRDJ_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin B12-dependent ribonucleotide reductase

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene names
Name:nrdJ
Ordered Locus Names:Bd1196
OrganismBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]
Taxonomic identifier264462 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase class-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Vitamin B12-dependent ribonucleotide reductase
PRO_0000231656

Regions

Region171 – 1722Substrate binding By similarity
Region376 – 3805Substrate binding By similarity
Region561 – 5655Substrate binding By similarity

Sites

Active site3761Proton acceptor By similarity
Active site3781Cysteine radical intermediate By similarity
Active site3801Proton acceptor By similarity
Binding site1271Substrate By similarity
Binding site2001Substrate; via amide nitrogen By similarity

Amino acid modifications

Disulfide bond172 ↔ 389Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MNP6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B9B5369BEE85DB1E

FASTA76484,408
        10         20         30         40         50         60 
MKKQTLHSPS YFVSGGKNPE SLFAWKNVNC EIRNRKGEVF FEMKNVEAPE GWSQLAIDIA 

        70         80         90        100        110        120 
ASKYFRKVGV PKTGHETSVR QMVDRVVKAI VASAIRQGGY FATRKEADIF AKELKFILLS 

       130        140        150        160        170        180 
QRGAFNSPVW FNAGLWEAYK AQSPSEHFAW DEKKKSIQAT KNAYERPQCS ACFIQSVDDS 

       190        200        210        220        230        240 
IESIFDLAKT EAKLFKYGSG TGSNFSKIRS KYEATGSGGK SSGLISFLEV LDKGAGAIKS 

       250        260        270        280        290        300 
GGTTRRAAKM VVVDIDHPEV LDFIDWKMRE EQKAHLLIAA GLSADFEGEA YRTVSGQNAN 

       310        320        330        340        350        360 
NSVRVSDAFM KAVEQERPWK LKARLTGKVL REIPAGEVWN RITRAAWMCA DPGIQFHDTI 

       370        380        390        400        410        420 
NKWHTCPETD IIHSSNPCSE YMFLDDSACN LASINLVKFL NDTGDFDFES FIHTARTLFV 

       430        440        450        460        470        480 
AQEILVDYSS YPTQRVAQNS HDYRPLGLGF ANLGSLLMRK GVAYDSDEGR AWAGALTSLM 

       490        500        510        520        530        540 
SGVAYLTSSE MARAKGPFAG YRKNSKSMLK VMKMHERALN QVAWKMLPAG IDKAVRNLWK 

       550        560        570        580        590        600 
GVLYNGEKYG YRNAQATVIA PTGTIGLLMD CDTTGIEPDF SLIKFKKLVG GGEIQIVNQS 

       610        620        630        640        650        660 
VDAALASLQY FEDERQAILK YVEENNSVVG APQMHPEDLP VFDTATAMPG QRVLSPESHV 

       670        680        690        700        710        720 
KMMAAVQPFI SGAISKTVNM PSTATEEDIS RIYFLAWKLG VKAVAVYRDG SKQSQPLNLK 

       730        740        750        760 
EKPKVVEEVG VPNFTMKCPE CGSDTVLTSG CYRCPNCGTT VGCS 

« Hide

References

[1]"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX842649 Genomic DNA. Translation: CAE79105.1.
RefSeqNP_968112.1. NC_005363.1.

3D structure databases

ProteinModelPortalQ6MNP6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264462.Bd1196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE79105; CAE79105; Bd1196.
GeneID2733233.
KEGGbba:Bd1196.
PATRIC21077002. VBIBdeBac73187_1082.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0209.
HOGENOMHOG000021771.
KOK00525.
OMAPRIEDIC.
OrthoDBEOG6H4K5F.

Family and domain databases

InterProIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
[Graphical view]
PfamPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02504. NrdJ_Z. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNRDJ_BDEBA
AccessionPrimary (citable) accession number: Q6MNP6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families