Reviewed, UniProtKB/Swiss-Prot Q6MNP6 (NRDJ_BDEBA)

Names and origin

Protein names

Recommended name:
    Vitamin B12-dependent ribonucleotide reductase
    EC=1.17.4.1
Alternative name(s):
    Ribonucleoside-diphosphate reductase nrdJ

Gene names

Name:	nrdJ
Ordered Locus Names:	Bd1196

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	class II ribonucleotide reductase activity Inferred from electronic annotation. Source: InterPro cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231656
Sites
	Active site	172	1	By similarity
	Active site	378	1	By similarity
	Active site	389	1	By similarity
	Active site	757	1	By similarity
	Active site	763	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MNP6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B9B5369BEE85DB1E	FASTA	764	84,408
	10 20 30 40 50 60 MKKQTLHSPS YFVSGGKNPE SLFAWKNVNC EIRNRKGEVF FEMKNVEAPE GWSQLAIDIA 70 80 90 100 110 120 ASKYFRKVGV PKTGHETSVR QMVDRVVKAI VASAIRQGGY FATRKEADIF AKELKFILLS 130 140 150 160 170 180 QRGAFNSPVW FNAGLWEAYK AQSPSEHFAW DEKKKSIQAT KNAYERPQCS ACFIQSVDDS 190 200 210 220 230 240 IESIFDLAKT EAKLFKYGSG TGSNFSKIRS KYEATGSGGK SSGLISFLEV LDKGAGAIKS 250 260 270 280 290 300 GGTTRRAAKM VVVDIDHPEV LDFIDWKMRE EQKAHLLIAA GLSADFEGEA YRTVSGQNAN 310 320 330 340 350 360 NSVRVSDAFM KAVEQERPWK LKARLTGKVL REIPAGEVWN RITRAAWMCA DPGIQFHDTI 370 380 390 400 410 420 NKWHTCPETD IIHSSNPCSE YMFLDDSACN LASINLVKFL NDTGDFDFES FIHTARTLFV 430 440 450 460 470 480 AQEILVDYSS YPTQRVAQNS HDYRPLGLGF ANLGSLLMRK GVAYDSDEGR AWAGALTSLM 490 500 510 520 530 540 SGVAYLTSSE MARAKGPFAG YRKNSKSMLK VMKMHERALN QVAWKMLPAG IDKAVRNLWK 550 560 570 580 590 600 GVLYNGEKYG YRNAQATVIA PTGTIGLLMD CDTTGIEPDF SLIKFKKLVG GGEIQIVNQS 610 620 630 640 650 660 VDAALASLQY FEDERQAILK YVEENNSVVG APQMHPEDLP VFDTATAMPG QRVLSPESHV 670 680 690 700 710 720 KMMAAVQPFI SGAISKTVNM PSTATEEDIS RIYFLAWKLG VKAVAVYRDG SKQSQPLNLK 730 740 750 760 EKPKVVEEVG VPNFTMKCPE CGSDTVLTSG CYRCPNCGTT VGCS

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842649 Genomic DNA. Translation: CAE79105.1.
RefSeq	NP_968112.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2733233.
GenomeReviews	Gene locus Bd1196 in contig BX842601_GR.
KEGG	bba:Bd1196.
NMPDR	fig|264462.1.peg.1082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MNP6.
OMA	KASINSE.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1196-MON.
BRENDA	1.17.4.1. 3459.
Family and domain databases
InterPro	IPR013344. RDP_Rdtase_AdoCbl-dep. IPR000788. Ribncl_red_lg_C. IPR013678. Ribonuc_red_2_N. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_BDEBA

Accession

Primary (citable) accession number: Q6MNP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)