Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6MNP6

- NRDJ_BDEBA

UniProt

Q6MNP6 - NRDJ_BDEBA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

adenosylcob(III)alaminBy similarityNote: 5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271SubstrateBy similarity
Binding sitei200 – 2001Substrate; via amide nitrogenBy similarity
Active sitei376 – 3761Proton acceptorBy similarity
Active sitei378 – 3781Cysteine radical intermediateBy similarity
Active sitei380 – 3801Proton acceptorBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:Bd1196
OrganismiBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Taxonomic identifieri264462 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio
ProteomesiUP000008080: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 764764Vitamin B12-dependent ribonucleotide reductasePRO_0000231656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi172 ↔ 389Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi264462.Bd1196.

Structurei

3D structure databases

ProteinModelPortaliQ6MNP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 1722Substrate bindingBy similarity
Regioni376 – 3805Substrate bindingBy similarity
Regioni561 – 5655Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021771.
KOiK00525.
OMAiAMDCGAT.
OrthoDBiEOG6H4K5F.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6MNP6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKQTLHSPS YFVSGGKNPE SLFAWKNVNC EIRNRKGEVF FEMKNVEAPE
60 70 80 90 100
GWSQLAIDIA ASKYFRKVGV PKTGHETSVR QMVDRVVKAI VASAIRQGGY
110 120 130 140 150
FATRKEADIF AKELKFILLS QRGAFNSPVW FNAGLWEAYK AQSPSEHFAW
160 170 180 190 200
DEKKKSIQAT KNAYERPQCS ACFIQSVDDS IESIFDLAKT EAKLFKYGSG
210 220 230 240 250
TGSNFSKIRS KYEATGSGGK SSGLISFLEV LDKGAGAIKS GGTTRRAAKM
260 270 280 290 300
VVVDIDHPEV LDFIDWKMRE EQKAHLLIAA GLSADFEGEA YRTVSGQNAN
310 320 330 340 350
NSVRVSDAFM KAVEQERPWK LKARLTGKVL REIPAGEVWN RITRAAWMCA
360 370 380 390 400
DPGIQFHDTI NKWHTCPETD IIHSSNPCSE YMFLDDSACN LASINLVKFL
410 420 430 440 450
NDTGDFDFES FIHTARTLFV AQEILVDYSS YPTQRVAQNS HDYRPLGLGF
460 470 480 490 500
ANLGSLLMRK GVAYDSDEGR AWAGALTSLM SGVAYLTSSE MARAKGPFAG
510 520 530 540 550
YRKNSKSMLK VMKMHERALN QVAWKMLPAG IDKAVRNLWK GVLYNGEKYG
560 570 580 590 600
YRNAQATVIA PTGTIGLLMD CDTTGIEPDF SLIKFKKLVG GGEIQIVNQS
610 620 630 640 650
VDAALASLQY FEDERQAILK YVEENNSVVG APQMHPEDLP VFDTATAMPG
660 670 680 690 700
QRVLSPESHV KMMAAVQPFI SGAISKTVNM PSTATEEDIS RIYFLAWKLG
710 720 730 740 750
VKAVAVYRDG SKQSQPLNLK EKPKVVEEVG VPNFTMKCPE CGSDTVLTSG
760
CYRCPNCGTT VGCS
Length:764
Mass (Da):84,408
Last modified:July 5, 2004 - v1
Checksum:iB9B5369BEE85DB1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842649 Genomic DNA. Translation: CAE79105.1.
RefSeqiNP_968112.1. NC_005363.1.
WP_011163707.1. NC_005363.1.

Genome annotation databases

EnsemblBacteriaiCAE79105; CAE79105; Bd1196.
GeneIDi2733233.
KEGGibba:Bd1196.
PATRICi21077002. VBIBdeBac73187_1082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842649 Genomic DNA. Translation: CAE79105.1 .
RefSeqi NP_968112.1. NC_005363.1.
WP_011163707.1. NC_005363.1.

3D structure databases

ProteinModelPortali Q6MNP6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264462.Bd1196.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE79105 ; CAE79105 ; Bd1196 .
GeneIDi 2733233.
KEGGi bba:Bd1196.
PATRICi 21077002. VBIBdeBac73187_1082.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021771.
KOi K00525.
OMAi AMDCGAT.
OrthoDBi EOG6H4K5F.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
    Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
    Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Entry informationi

Entry nameiNRDJ_BDEBA
AccessioniPrimary (citable) accession number: Q6MNP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3