Q6MNF1 (ACSA_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Bd1306

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065272
Sites
	Active site	514	1	By similarity
Amino acid modifications
	Modified residue	606	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MNF1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D2A8EF1D01D9FF67	FASTA	645	72,415
	10 20 30 40 50 60 MHKELYPVNS EVAKKAWIDE AKYKAMYERS IQDNEAFWAE QAERLDWIKK WDKVKEVSFK 70 80 90 100 110 120 KPVSIKWYLG GKMNVSYNCV DRHLKTRGDK TALLWEADNP STPSRKITYK ELHLEVCRFA 130 140 150 160 170 180 NVLKKMGVKK GDVVTIYMPM IPDTAVAMLA CARIGAVHSV VFAGFSPDSI SDRILDGQCR 190 200 210 220 230 240 FVITGDAGFR GSKVVALKEN IDKALVKTPD VQKVLVVKYA GTTVDMKPGR DLWYHEEVKT 250 260 270 280 290 300 VSDQCEPEPM DAEDPLFILY TSGSTGKPKG VMHTTGGYLV YASMTHQYVF DYHEDDIYWC 310 320 330 340 350 360 SADVGWVTGH SYIVYGPLAN GATSLFFEGV PNYPTPSRFW EVVDKHKVTI FYTSPTAIRS 370 380 390 400 410 420 LMREGDAAVK TTSRKTLRLL GSVGEPINPE AWAWYHDVVG EGRCPVVDTW WQTETGGILI 430 440 450 460 470 480 TPLPGAIAQK PGSATLPFFG VQPKLLTNEG QEIHGPGEGV LVIADSWPGQ MRTVYRNHER 490 500 510 520 530 540 FEDTYFSNYP GYYFTGDGCR RDQDGYYWIT GRVDDVINVS GHRLGTAEIE SALVAHHKVA 550 560 570 580 590 600 EAAVVGYPHD IKGQGIYAFV TLKSGETASE ELRKELIQTV RKEIGPIATP DLIQWAPRLP 610 620 630 640 KTRSGKIMRR ILRKIAENHP DQLGDTTTLS EPAVVQELVD NRMNR

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842649 Genomic DNA. Translation: CAE79201.1.
RefSeq	NP_968208.1. NC_005363.1.
3D structure databases
HSSP	HSSP built from PDB template 1PG4 based on UniProtKB Q8ZKF6.
ProteinModelPortal	Q6MNF1.
SMR	Q6MNF1. Positions 2-642.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2734497.
GenomeReviews	Gene locus Bd1306 in contig BX842601_GR.
KEGG	bba:Bd1306.
NMPDR	fig|264462.1.peg.1178.
PATRIC	21077202. VBIBdeBac73187_1181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	KGKPYML.
PhylomeDB	Q6MNF1.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1306-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BDEBA

Accession

Primary (citable) accession number: Q6MNF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program