Reviewed, UniProtKB/Swiss-Prot Q6MN06 (PURA_BDEBA)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Bd1460

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000224259
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	141	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MN06-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 94529BF30BE46BCB	FASTA	432	46,897
	10 20 30 40 50 60 MSGVVVVGAQ WGDEGKGKLI DVFAEKADMV VRYQGGANAG HTLVVNGQKT VLHLVPSGIL 70 80 90 100 110 120 RPETTCVIAS GVVIDVFSIR DEIKKLKDTG FLQNPKQLLI SDTATLILPY HKALDAAREA 130 140 150 160 170 180 ALSDGKIGTT GKGIGPAYED RASRRAILFG DLFDKDNLKK KLELALTEKN FMLENYYKGS 190 200 210 220 230 240 TFKADDLIKD LLAVAEELAP YRTKDTSLFI SKSLKSGKRV LFEGAQGTML DILHGTYPFV 250 260 270 280 290 300 TSSSTLASNA CASAGIGPAS VQKVIGVFKA YTTRVGSGPF PTELNDEIGK KIQADGHEFG 310 320 330 340 350 360 STTGRSRRCG WLDLVALKYA IRVNGITNLA MMKLDVLTGH DRIGVCTAYK LNGEIITDLP 370 380 390 400 410 420 TSPYELEKVE PVIEWIPGWT QDLTKVKTLS DLPRPTTNYI DYLGSQLGTP IDVISVGPGR 430 EQTLWVKPLF NN

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842649 Genomic DNA. Translation: CAE79346.1.
RefSeq	NP_968353.1.
3D structure databases
SMR	Q6MN06. Positions 3-431.
ModBase	Search...
Genome annotation databases
GeneID	2735591.
KEGG	bba:Bd1460.
NMPDR	fig|264462.1.peg.1323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG658237.
OMA	IPVCVAY.
PhylomeDB	Q6MN06.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1460-MONOMER.
BRENDA	6.3.4.4. 3459.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. Divergent sequence. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BDEBA

Accession

Primary (citable) accession number: Q6MN06

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)