Q6MML0 (SYT_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Bd1617

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 656	656	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100943
Regions
	Region	251 – 542	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	342	1	Zinc; catalytic By similarity
	Metal binding	393	1	Zinc; catalytic By similarity
	Metal binding	519	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MML0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 50E4C983EC1268BC	FASTA	656	75,228
	10 20 30 40 50 60 MLMSQITIIL PDNSTKVFDH EPTALEVAMS IGPRLAKETL GAKLNGSTEI SDLRTVLKDQ 70 80 90 100 110 120 TKVALVTTKS PESVEVVRHS CAHIMAQAIQ DIWPEVKVTI GPVIDNGFYY DFDSPFAFTE 130 140 150 160 170 180 EHFEKIEKKM SEIVSKDLPI HREDWPIQKA IETFKGMNER FKVELIEDLA KKGETTVGIY 190 200 210 220 230 240 FNGTSWFDLC RGPHIQSTGQ IKAFKLLSVA GAYWRGDEKN AQLQRVYATA FNDKKDLETY 250 260 270 280 290 300 LHNIEEAKKR DHRKLGKELG MFYFNELAPG SPFFTGKGAT VYNSLQTYLR ELYFETGYQE 310 320 330 340 350 360 VITPQIFDVN LFHTSGHYQN YKENMFFTKV DERDFASKPM NCPSHCLLYN SEKYSYRDLP 370 380 390 400 410 420 IKMADFGRLH RYEKSGAMHG LTRVRTFCQD DAHIFCRMDQ LQEEIAKFMN LLNRVYDKLG 430 440 450 460 470 480 MGNYKIFLST RPDNRMGSEE YWDMAEGALA EALKSLNLPF ELNPGDGAFY GPKLDIMFVD 490 500 510 520 530 540 ALNRPWQLGT LQVDPNLPQA FDLKYTGEDN KEHRPVMLHR AILGSLERFI GVYLEHTAGH 550 560 570 580 590 600 LPPWMMPVQV AILNVTDRVN SFCEELQNSL KGHSVRVEFD RRNEKLNYKI REAQLQKIPY 610 620 630 640 650 MIIVGDKEAE SRTVSLRLRD GSEHKGLTVD QVMNLITTDI KTRSLQSSLA KSATTN

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842650 Genomic DNA. Translation: CAE79494.1.
RefSeq	NP_968501.1. NC_005363.1.
3D structure databases
ProteinModelPortal	Q6MML0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2735370.
GenomeReviews	Gene locus Bd1617 in contig BX842601_GR.
KEGG	bba:Bd1617.
NMPDR	fig|264462.1.peg.1471.
PATRIC	21077782. VBIBdeBac73187_1467.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	VGSDALW.
PhylomeDB	Q6MML0.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1617-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BDEBA

Accession

Primary (citable) accession number: Q6MML0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program