Reviewed, UniProtKB/Swiss-Prot Q6MMJ2 (SYFA_BDEBA)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Bd1637

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126668
Sites
	Metal binding	254	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MMJ2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4377438402A486DE	FASTA	341	38,244
	10 20 30 40 50 60 MSTTKLDSIK DNALAAFKAA PSSKDLYDLK VQYLGKSGSL TEIMKEMASL PKEEKPLFGK 70 80 90 100 110 120 KVNEVKQLLE AAYTEAEDAL KKKEISAKMA AEEIDMTLPA FSQPKGTAHP VNIVVEEIFT 130 140 150 160 170 180 VMSRLGYSIR TGPMIEKDYY NFEALNIPAD HPARDMADTF FVDKTHVLRT HTSPIQIHSL 190 200 210 220 230 240 ENEELPLRVI GTGPVFRCDS DISHLPNFHQ IEALCVDEKI SMADLKGTIS FFVREFFGPG 250 260 270 280 290 300 LKTRFRPSYF PFTEPSAEVD CSCPICKGKG CSLCKQSGWI EIGGCGLVNP KVFQAAKIEY 310 320 330 340 PKWQGFAFGF GVERMAIIKY GIEDIRLFPE NDVRFLRQFV K

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842650 Genomic DNA. Translation: CAE79512.1.
RefSeq	NP_968519.1.
3D structure databases
HSSP	HSSP built from PDB template 1B7Y based on UniProtKB P27001.
ModBase	Search...
Genome annotation databases
GeneID	2735390.
GenomeReviews	Gene locus Bd1637 in contig BX842601_GR.
KEGG	bba:Bd1637.
NMPDR	fig|264462.1.peg.1489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MMJ2.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1637-MON.
BRENDA	6.1.1.20. 3459.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BDEBA

Accession

Primary (citable) accession number: Q6MMJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)