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Q6MMA3 (SYM_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:Bd1733
OrganismBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]
Taxonomic identifier264462 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length687 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 687687Methionine--tRNA ligase HAMAP MF_00098
PRO_0000139104

Regions

Domain585 – 687103tRNA-binding
Motif14 – 2411"HIGH" region HAMAP MF_00098
Motif329 – 3335"KMSKS" region HAMAP MF_00098

Sites

Metal binding1451Zinc By similarity
Metal binding1481Zinc By similarity
Metal binding1581Zinc By similarity
Metal binding1611Zinc By similarity
Binding site3321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MMA3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7975C9CA2D918A74

FASTA68777,726
        10         20         30         40         50         60 
MNDKRKILIT CALPYANGYI HLGHLVEYLQ ADFWARFQNM RGNECVFICA DDTHGTPIMV 

        70         80         90        100        110        120 
KARELGITPE ALIAQSYKEH TQDFADFQVQ FSHFGSTNSE ENRLLCEYFY KKMQEGNHTR 

       130        140        150        160        170        180 
SQPIQQMYCN HDKMFLPDRF VKGTCPKCGA KEQYGDSCDV CASTYSPSDM KDVHCSLCGT 

       190        200        210        220        230        240 
APVMKDSESI FFKLNDFKQY LEEWIPKHCS PEISKKMLEW FNEDLKDLDI SRDEPYFGFA 

       250        260        270        280        290        300 
IPGTNNKKFF YVWVDAPMGY MSTTEQWAKS QGKTLKDIWQ DPSREIYHFI GKDIARFHTI 

       310        320        330        340        350        360 
FWPAFLKAAE FRSPNQVFVH GHLMVNGEKM SKSKGTFIAA RTYLNHLNPE YLRYYYSTKL 

       370        380        390        400        410        420 
SSSVDDIDLN LEDFTNRVNS ELVGKITNLG SRGGQMLKKK MDGKMSVPDA EGKKLIEHAQ 

       430        440        450        460        470        480 
KTAESIAAHY EARDFAKALG EIRGLADDAN KYFDEKAPWK TLEADPEGTK QVITTTLNMF 

       490        500        510        520        530        540 
RMLAIYLKPV LPFYSQKVAK LLGEKDYVWS DLNTVLTNRE INDYEHLATR IEADKVKAMV 

       550        560        570        580        590        600 
EEGRKINEEI QAAKKAASTA KPAAAPAPAA AATAGDRPAE IEFADFDKVD LRIGQVIEAE 

       610        620        630        640        650        660 
EIKEADKLLR LKIDIGEGQI RQIISGIKAA YKPEQLVGRK VLVCVNLKPR KMKFGMSEGM 

       670        680 
VLAAGTGGSD LFVLSADDGA QVGQRVK

« Hide

References

[1]"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
Science 303:689-692(2004) [PubMed: 14752164] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842650 Genomic DNA. Translation: CAE79602.1.
RefSeqNP_968609.1. NC_005363.1.

3D structure databases

ProteinModelPortalQ6MMA3.
SMRQ6MMA3. Positions 138-163.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2735203.
GenomeReviewsGene locus Bd1733 in contig BX842601_GR.
KEGGbba:Bd1733.
NMPDRfig|264462.1.peg.1579.
PATRIC21077996. VBIBdeBac73187_1573.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG721667.
OMAEEKTRNV.
PhylomeDBQ6MMA3.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBBAC264462:BD1733-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BDEBA
AccessionPrimary (citable) accession number: Q6MMA3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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