Q6ML97 (TRMD_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Bd2119

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060333
Regions
	Region	131 – 136	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	111	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ML97 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9BA78C3993DA90D9	FASTA	275	30,752
	10 20 30 40 50 60 MLKIDVITLF PEMIQGAVSH GVLGQALKGD LLSVQAHTPR EFASDKHRTV DDRPFGGGDG 70 80 90 100 110 120 MIMLSETLEK SIQKVQHKNS KVIYLSPQGS VLTDDKAREL SQAEHLVLIC GRYGGIDQRI 130 140 150 160 170 180 INTYVDEEIS IGDYVLSGGE LGALVVIDAL SRFIPGVLGH VDSADKDSFS EGLLEHPNFT 190 200 210 220 230 240 RPREYLGQDV PEVLLSGNHK LIADWKEKVS ALVTLKKRPD LFIIYLAEER EKYRALKKKK 250 260 270 TAEPLKELFK FWQQLSPQDR EVLGLEDLNE ESFNG

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842651 Genomic DNA. Translation: CAE79960.1.
RefSeq	NP_968967.1. NC_005363.1.
3D structure databases
ProteinModelPortal	Q6ML97.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2733717.
GenomeReviews	Gene locus Bd2119 in contig BX842601_GR.
KEGG	bba:Bd2119.
NMPDR	fig|264462.1.peg.1938.
PATRIC	21078734. VBIBdeBac73187_1933.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	NRYDLYL.
PhylomeDB	Q6ML97.
Enzyme and pathway databases
BioCyc	BBAC264462:BD2119-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR000910. HMG_HMG1/HMG2. IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 1 hit. G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BDEBA

Accession

Primary (citable) accession number: Q6ML97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program