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Q6MKX0

- SYI_BDEBA

UniProt

Q6MKX0 - SYI_BDEBA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Bd2257
Organism
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Bd2257
OrganismiBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Taxonomic identifieri264462 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio
ProteomesiUP000008080: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098523Add
BLAST

Proteomic databases

PRIDEiQ6MKX0.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi264462.Bd2257.

Structurei

3D structure databases

ProteinModelPortaliQ6MKX0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionUniRule annotation
Add
BLAST
Motifi603 – 6075"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MKX0-1 [UniParc]FASTAAdd to Basket

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MTNANTRSTP YSAVKPDVNL AKQEETILDF WDQEKIFAQS LNPEGKKTYS     50
FYDGPPFATG LPHYGHLLAG VLKDVVPRYW TMKGYTVPRR FGWDCHGLPV 100
EYEINKTHKI ESRKDVFKMG VANYNDACRS IVKRYSTEWK TTVRRVGRWV 150
DMENPYFTMD VSFMQSVWWV FQQLFNKGLI YEGYKVVPYS VGISTSLSNF 200
EANQNYKMVQ DPAITVMFKL VNQPDTAIMA WTTTPWTLPS NLALAVGNDI 250
EYVKVQEKAT GRKLIMAQAL LSSVFKKADE EVEVLQMMKG TELVGLTYEP 300
LFPYFGDRAD KGAFRIISSD HVTTESGTGV VHMAPAFGEE DYYACAKAGI 350
PMVNPVDDDG MFTMEVPDYA GKRVKEADKD IIADLKKRGN LFKQDTIQHS 400
YPFCYRSDTP LIYRAVSSWF VAVEKIKEEL IANNKQTSWV PDHLRDGRFG 450
NWLEGARDWA ISRNRFWGTP LPIWRNAEGE VMCIGSRAEL EKLSGQKVDD 500
LHIEFVDKIT IPSPTGKSPL KRVDGVLDCW FESGSMPYAQ WGYPETSVED 550
FKKAFPADFI AEGLDQTRGW FYTLSIIGTA LFNQAPFKNV VVNGLVLAED 600
GRKMSKSLKN YPDPMEVLNQ HGADALRLYL IDSPVVKAQE LKFSEKGVYD 650
IVRKILLRWW NSYSFFANYA NIDGFVPKGD AKKSPNILDQ WVLSRLNGLI 700
ANTHKEMDAY RLYNVVPHLL QFIEDLTNTY IRFNRSLFWQ DGMPETKRYA 750
YETLHEVLVT LSRLMAPFAP FMSEVTYKNL AQVLKDKKDS VHLESFPTAD 800
LSMLRPELEE AVKAMDTLVT LGRNHREKIG VKAKIPLNEI KIIHRSAELL 850
ETLKKFEPFF VDELNFRKVV YNPNEDQFVQ VTAKANFPVL GKRLGPKMKA 900
VGAGIMSMSL ENILKLEGGG VVVIEGEEIS LSDVEIRRAP KGDNANLSVH 950
QIVSIEVDPT VTPEQEREGL AREIMRKIQV ARKTADFQMD DKITLEIACD 1000
GALLDALNAH KDMITGETLT KNLNILALTA EPNGKHTETS DIDGQVIKIG 1050
VTNLPR 1056
Length:1,056
Mass (Da):119,738
Last modified:July 5, 2004 - v1
Checksum:i2B200E2A1FCA95CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX842652 Genomic DNA. Translation: CAE80087.1.
RefSeqiNP_969094.1. NC_005363.1.

Genome annotation databases

EnsemblBacteriaiCAE80087; CAE80087; Bd2257.
GeneIDi2733851.
KEGGibba:Bd2257.
PATRICi21079000. VBIBdeBac73187_2065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX842652 Genomic DNA. Translation: CAE80087.1 .
RefSeqi NP_969094.1. NC_005363.1.

3D structure databases

ProteinModelPortali Q6MKX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264462.Bd2257.

Proteomic databases

PRIDEi Q6MKX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE80087 ; CAE80087 ; Bd2257 .
GeneIDi 2733851.
KEGGi bba:Bd2257.
PATRICi 21079000. VBIBdeBac73187_2065.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
    Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
    Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Entry informationi

Entry nameiSYI_BDEBA
AccessioniPrimary (citable) accession number: Q6MKX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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