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Q6MKX0

- SYI_BDEBA

UniProt

Q6MKX0 - SYI_BDEBA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bd2257
OrganismiBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Taxonomic identifieri264462 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio
ProteomesiUP000008080: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Isoleucine--tRNA ligasePRO_0000098523Add
BLAST

Proteomic databases

PRIDEiQ6MKX0.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264462.Bd2257.

Structurei

3D structure databases

ProteinModelPortaliQ6MKX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MKX0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNANTRSTP YSAVKPDVNL AKQEETILDF WDQEKIFAQS LNPEGKKTYS
60 70 80 90 100
FYDGPPFATG LPHYGHLLAG VLKDVVPRYW TMKGYTVPRR FGWDCHGLPV
110 120 130 140 150
EYEINKTHKI ESRKDVFKMG VANYNDACRS IVKRYSTEWK TTVRRVGRWV
160 170 180 190 200
DMENPYFTMD VSFMQSVWWV FQQLFNKGLI YEGYKVVPYS VGISTSLSNF
210 220 230 240 250
EANQNYKMVQ DPAITVMFKL VNQPDTAIMA WTTTPWTLPS NLALAVGNDI
260 270 280 290 300
EYVKVQEKAT GRKLIMAQAL LSSVFKKADE EVEVLQMMKG TELVGLTYEP
310 320 330 340 350
LFPYFGDRAD KGAFRIISSD HVTTESGTGV VHMAPAFGEE DYYACAKAGI
360 370 380 390 400
PMVNPVDDDG MFTMEVPDYA GKRVKEADKD IIADLKKRGN LFKQDTIQHS
410 420 430 440 450
YPFCYRSDTP LIYRAVSSWF VAVEKIKEEL IANNKQTSWV PDHLRDGRFG
460 470 480 490 500
NWLEGARDWA ISRNRFWGTP LPIWRNAEGE VMCIGSRAEL EKLSGQKVDD
510 520 530 540 550
LHIEFVDKIT IPSPTGKSPL KRVDGVLDCW FESGSMPYAQ WGYPETSVED
560 570 580 590 600
FKKAFPADFI AEGLDQTRGW FYTLSIIGTA LFNQAPFKNV VVNGLVLAED
610 620 630 640 650
GRKMSKSLKN YPDPMEVLNQ HGADALRLYL IDSPVVKAQE LKFSEKGVYD
660 670 680 690 700
IVRKILLRWW NSYSFFANYA NIDGFVPKGD AKKSPNILDQ WVLSRLNGLI
710 720 730 740 750
ANTHKEMDAY RLYNVVPHLL QFIEDLTNTY IRFNRSLFWQ DGMPETKRYA
760 770 780 790 800
YETLHEVLVT LSRLMAPFAP FMSEVTYKNL AQVLKDKKDS VHLESFPTAD
810 820 830 840 850
LSMLRPELEE AVKAMDTLVT LGRNHREKIG VKAKIPLNEI KIIHRSAELL
860 870 880 890 900
ETLKKFEPFF VDELNFRKVV YNPNEDQFVQ VTAKANFPVL GKRLGPKMKA
910 920 930 940 950
VGAGIMSMSL ENILKLEGGG VVVIEGEEIS LSDVEIRRAP KGDNANLSVH
960 970 980 990 1000
QIVSIEVDPT VTPEQEREGL AREIMRKIQV ARKTADFQMD DKITLEIACD
1010 1020 1030 1040 1050
GALLDALNAH KDMITGETLT KNLNILALTA EPNGKHTETS DIDGQVIKIG

VTNLPR
Length:1,056
Mass (Da):119,738
Last modified:July 5, 2004 - v1
Checksum:i2B200E2A1FCA95CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842652 Genomic DNA. Translation: CAE80087.1.
RefSeqiNP_969094.1. NC_005363.1.

Genome annotation databases

EnsemblBacteriaiCAE80087; CAE80087; Bd2257.
GeneIDi2733851.
KEGGibba:Bd2257.
PATRICi21079000. VBIBdeBac73187_2065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX842652 Genomic DNA. Translation: CAE80087.1 .
RefSeqi NP_969094.1. NC_005363.1.

3D structure databases

ProteinModelPortali Q6MKX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264462.Bd2257.

Proteomic databases

PRIDEi Q6MKX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE80087 ; CAE80087 ; Bd2257 .
GeneIDi 2733851.
KEGGi bba:Bd2257.
PATRICi 21079000. VBIBdeBac73187_2065.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
    Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
    Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Entry informationi

Entry nameiSYI_BDEBA
AccessioniPrimary (citable) accession number: Q6MKX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3