Q6MKR6 (SYE_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Bd2319

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119514
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00022_B
	Motif	259 – 263	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	262	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MKR6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 93CFE5194BA53A1E	FASTA	490	55,717
	10 20 30 40 50 60 MSSKISPHVR VRFAPSPTGY LHVGGARTAL YNYLFAKKNG GEFILRIEDT DEARSTQESL 70 80 90 100 110 120 RGVVDDLVWL GLKWDEGVDP VTLKDVGPNG PYRQSERLHI YKEIADQLLK EGKAYYCFMT 130 140 150 160 170 180 EEDIEKQKAA AGSFAHLVSP YQDWTLDQAL ERLKTGDKAV VRFKTKNLVK DYIFTDLVRG 190 200 210 220 230 240 EVKFPSDMVG DFVLLRSDGM PVYNFCCVVD DHLMKMTHVF RAEEHLPNTL RQLMIYEAMN 250 260 270 280 290 300 WPTPEFGHMA LILDEDRQKL SKRKGAVACG QLKDEGYLAS AVLNFIALLG WSDPQGREIL 310 320 330 340 350 360 SVKDMLEVFD ISRLNPSGAI FDRVKFKWMN AQHLRALPNA ELWQAVAPFL ARENMDLPTD 370 380 390 400 410 420 PAWQSKSLDL FKPYMEILAD AIELYRPLND KSYVILPEAD ETLKWESTKA VLTSWKNLLQ 430 440 450 460 470 480 AHPSDYMTEE EFLKMQDEVK NQTGSKGKHL FMPIRVAVIG KPHGAELKTL VPLMKKSSLV 490 ARAEQALAKV

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842652 Genomic DNA. Translation: CAE80141.1.
RefSeq	NP_969148.1. NC_005363.1.
3D structure databases
ProteinModelPortal	Q6MKR6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2735761.
GenomeReviews	Gene locus Bd2319 in contig BX842601_GR.
KEGG	bba:Bd2319.
NMPDR	fig|264462.1.peg.2119.
PATRIC	21079114. VBIBdeBac73187_2120.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	ANFNDES.
PhylomeDB	Q6MKR6.
Enzyme and pathway databases
BioCyc	BBAC264462:BD2319-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BDEBA

Accession

Primary (citable) accession number: Q6MKR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program