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Q6MKB6 (RUVC_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Bd2489
OrganismBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]
Taxonomic identifier264462 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBdellovibrionaceaeBdellovibrio

Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168168Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000225123

Sites

Metal binding91Magnesium By similarity
Metal binding691Magnesium By similarity
Metal binding1411Magnesium By similarity
Metal binding1441Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MKB6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A1E06F11AB33BBD5

FASTA16818,158
        10         20         30         40         50         60 
MSLVILGVDP GSRITGFGVV RVANGKIEHI NHGVIVMDGD DAFPRRMTEL GSAFREVMEK 

        70         80         90        100        110        120 
YKPEQVVIEK IFLGKNADSA FKLGHARGVI MYEAGLGGAE VQEYATRSVK KGVTGNGGAS 

       130        140        150        160 
KEDVQAILKV MLSLKTISRI DASDALAMAC YHAFEMKKKA LMQRAVSL 

« Hide

References

[1]"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective."
Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.
Science 303:689-692(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX842652 Genomic DNA. Translation: CAE80292.1.
RefSeqNP_969299.1. NC_005363.1.

3D structure databases

ProteinModelPortalQ6MKB6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264462.Bd2489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE80292; CAE80292; Bd2489.
GeneID2735966.
KEGGbba:Bd2489.
PATRIC21079428. VBIBdeBac73187_2277.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_BDEBA
AccessionPrimary (citable) accession number: Q6MKB6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families