Reviewed, UniProtKB/Swiss-Prot Q6MJE4 (MAO1_BDEBA)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	Bd2834

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160213
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MJE4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6DAB2AFA0710B994	FASTA	565	62,686
	10 20 30 40 50 60 METDNKYARP VYIPYSGTVL LEMSLLNKGS AFSEEERTHL NLHGLVPQTI ETIEEQAERV 70 80 90 100 110 120 YSQYRLLKAD IDKHVYLRNL QDTNETLFYH LVVNHLTEMM PIIYTPVVGE ACEHFSEIYR 130 140 150 160 170 180 ASRGLFISYP NKDRIDEMLR NVTKQKVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDVGTN NQALLNDPLY MGWRQPRITG KEYEDFVDAF VQAVKRRWPD 250 260 270 280 290 300 VLLQFEDFAQ KNASPILKRY RDEICSFNDD IQGTAAVTVG CLIAASRANG GRLRDQRVVF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAEMKH EGLSDEQARQ RVFMVDRFGL LTDKMPNLLE FQSPLVQKTE 370 380 390 400 410 420 TLAGWEVTND EISLLDTVRN AKPTILIGVS AQPGLFTEEV IRTMQSHCEH PIVMPLSNPT 430 440 450 460 470 480 SRVEATPADI LRWTDGKALV ATGSPFMPVH MNDQIYPIAQ CNNSYIFPGI GLGVLACGAR 490 500 510 520 530 540 RVTDSMLMAA SSALADCSPL AQNGKGALLP ELSQIEKVSR TIAFAVAKAA QADEVAVKVS 550 560 DEELLSLIDK NYWYPKYRSY KHASS

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842653 Genomic DNA. Translation: CAE80616.1.
RefSeq	NP_969623.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2736386.
GenomeReviews	Gene locus Bd2834 in contig BX842601_GR.
KEGG	bba:Bd2834.
NMPDR	fig|264462.1.peg.2595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MJE4.
Enzyme and pathway databases
BioCyc	BBAC264462:BD2834-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_BDEBA

Accession

Primary (citable) accession number: Q6MJE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)