Reviewed, UniProtKB/Swiss-Prot Q6MIF8 (MURD_BDEBA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Bd3200

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108973
Regions
	Nucleotide binding	120 – 126	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6MIF8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 746915D646499FD1	FASTA	462	51,466
	10 20 30 40 50 60 MYKEYSDLKD KRILVVGLGK TGVSLAHFLT KHGAQVTVTD HKSKPELSVQ LEQLGELPIK 70 80 90 100 110 120 FELGGHSPKT FIAQDLVILS PGVPSNLKIF DYARSQGIKI TGEFEFSAGF IKEPIIGLTG 130 140 150 160 170 180 TNGKTTVAKI TEAILTESGV KTWVGGANEK PLVDYLRLDD KAQVVIAEVS SFMLEHCDTF 190 200 210 220 230 240 NPGNIVFTNL AENHLDRYRS MEEYVNAKRR IFKNTNQATT SILNADDNAV VELARDPAVQ 250 260 270 280 290 300 RGRIFYFSRK PALEPQIMNI GGAVNIGDEI RVRTGPEIES FNIKGMKMRG KHSVENIMAA 310 320 330 340 350 360 ILASREHGAT REAVQKVINT FTGLPHRIEY VRKVGGVMFY NDSKATNVHA VLRALDTFDE 370 380 390 400 410 420 NVILIAGGKD TNLNYEPLRT SVKRKVKTLI LVGEAKERIN RDLGDFSETF LIGTFEEAVL 430 440 450 460 IAYQKSRIGD VVLLSPGCSS FDMFDSFEER GDYFKEIVRK FH

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842654 Genomic DNA. Translation: CAE80955.1.
RefSeq	NP_969962.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2736817.
GenomeReviews	Gene locus Bd3200 in contig BX842601_GR.
KEGG	bba:Bd3200.
NMPDR	fig|264462.1.peg.2935.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MIF8.
OMA	DSKATNF.
Enzyme and pathway databases
BioCyc	BBAC264462:BD3200-MON.
BRENDA	6.3.2.9. 3459.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BDEBA

Accession

Primary (citable) accession number: Q6MIF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)