Reviewed, UniProtKB/Swiss-Prot Q6MID2 (TYSY_BDEBA)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Bd3230

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140936
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MID2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8122942856B5E102	FASTA	264	30,443
	10 20 30 40 50 60 MKQYHDLIKT VLEHGVKKED RTGTGTISMF GYQMRYNLQE GFPLLTTKKL HTRSIFHELL 70 80 90 100 110 120 WFLKGETNIK YLHDNKVTIW DEWADENGNL GPVYGKQWRS WETADGRTID QITNVVEQIK 130 140 150 160 170 180 KNPNSRRMLV VAFNPGDVDK MALPPCHAFF QFYVANGKLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTHMIAQ VCDLEVGDFV HTLGDAHLYL NHLEQANLQL TREFRPLPQL KLNPAKKDLF 250 260 DFTYEDIEII GYDPHPAIKA EVAV

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842655 Genomic DNA. Translation: CAE78047.1.
RefSeq	NP_969988.1.
3D structure databases
HSSP	HSSP built from PDB template 1AXW based on UniProtKB P00470.
ModBase	Search...
Genome annotation databases
GeneID	2736851.
GenomeReviews	Gene locus Bd3230 in contig BX842601_GR.
KEGG	bba:Bd3230.
NMPDR	fig|264462.1.peg.2961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6MID2.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BBAC264462:BD3230-MON.
BRENDA	2.1.1.45. 3459.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BDEBA

Accession

Primary (citable) accession number: Q6MID2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)