Q6MI59 (SYD_BDEBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Bd3311

Organism

Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) [Complete proteome] [HAMAP]

Taxonomic identifier

264462 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110833

Sequences

	Sequence		Length	Mass (Da)
	Q6MI59 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 273C93A64D23BA1F	FASTA	642	72,343
	10 20 30 40 50 60 MPLVEGPLYR FCYGEYKNMV FDCNGTICKS GAHEQGRHMK FVKELKRTHY CGSLGVSQAG 70 80 90 100 110 120 QKVVLMGWVD VRRDHGSLVF IDLRDREGIV QVVLDPNKAE TASSKNLRGE FVLAVEGVVR 130 140 150 160 170 180 ARPDGMKNAK IKTGEVEVEA IRCEILNESA VPPFQVSDTN VNEMLRLKYR YLDLRSARLS 190 200 210 220 230 240 SHLITRHKVA QLVRRFLSDN GFLEVETPIL YKSTPEGARD YLVPSRVNPG HFYALPQSPQ 250 260 270 280 290 300 TLKQLLMISG YDRYFQIARC FRDEDLRADR QPEFSQIDME MSYIDQEDIM EMNEKLLRTI 310 320 330 340 350 360 WKEIKGIDVG AIPRMTYQEA MDRYGIDKPD TRFGVEIKDL KSIVTGSGFK VFDDVLARGG 370 380 390 400 410 420 IVRGIAAPKG GSYSRGQLDK LTDMAKRAGA KGLVWIKSEA DGTLSSSVSK FFSPEKLAEM 430 440 450 460 470 480 FKACGGEAGD CALVVADDYD TACAALSTLR LHLGRELNLI DNSKYKFLWV VDFPLLEYSP 490 500 510 520 530 540 DEKRWVARHH PFTSPKDEFA QDLVNNNEAA YGKMLAKAYD LVCNGYEMGG GSIRIYRNEI 550 560 570 580 590 600 QQAMFRLLGM SEEETQHKFG FFLEALKYGT PPHGGIAWGM DRLVMLLCET DAIREVIAFP 610 620 630 640 KTAKATDLMS DCPSEVNRDQ LAEVGVRLST LAEKHLEDLK KS

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842655 Genomic DNA. Translation: CAE78121.1.
RefSeq	NP_970062.1. NC_005363.1.
3D structure databases
ProteinModelPortal	Q6MI59.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2736950.
GenomeReviews	Gene locus Bd3311 in contig BX842601_GR.
KEGG	bba:Bd3311.
NMPDR	fig|264462.1.peg.3035.
PATRIC	21080950. VBIBdeBac73187_3030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	Q6MI59.
Enzyme and pathway databases
BioCyc	BBAC264462:BD3311-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. Divergent sequence. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BDEBA

Accession

Primary (citable) accession number: Q6MI59

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program