Reviewed, UniProtKB/Swiss-Prot Q6MH10 (AMPA_BDEBA)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bd3755

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165723
Sites
	Active site	277	1	Potential
	Active site	351	1	Potential
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	270	1	Manganese 1 By similarity
	Metal binding	270	1	Manganese 2 By similarity
	Metal binding	288	1	Manganese 2 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	349	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MH10-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3C387D0431287A1C	FASTA	500	53,262
	10 20 30 40 50 60 MAFNLLNKDI DTLTCPALVV FSKSSSQKDK LAKVTHSEIH KALLPSLEEK TISGKHQETV 70 80 90 100 110 120 VFREFSFKGF RHVIVVGLGK ENEITHESVR QSMASAYEAI KALNVTEAAV HFDGITAGKK 130 140 150 160 170 180 DAADFAKATA EGLILTSYVF NELMSGKKET KEINVHVVSK MGNDKAVKAA FNEGAILGSV 190 200 210 220 230 240 VNFSRRLGDL PGNLMTPTIL ADSAVEGAKG IANLKVTVWD KARIKKEKMG GLLGVSNGSD 250 260 270 280 290 300 QEPRFIIMEY KGAAASKKPV CFVGKGLTFD CGGISIKPGA GMEEMKYDMC GGANVIGTLL 310 320 330 340 350 360 AIAKLKLKVN AVGLVASTEN LINGSATKPG DVHTARNGKT FEVNNTDAEG RLILADALSY 370 380 390 400 410 420 ATELQPQMIV DAATLTGAMV IALGNTHTGY FTRNSALKTK VEKAAAESGE WVWNMPLTDF 430 440 450 460 470 480 HVKDMKGTYA DLSNISSGKG AGSATAAAFL EQFVGEGIPW AHFDIAGTGW AVGNRLPYCP 490 500 KKGASGAMIR TFVEIAKQYT

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842656 Genomic DNA. Translation: CAE81117.1.
RefSeq	NP_970463.1.
3D structure databases
SMR	Q6MH10. Positions 1-498, 40-500.
ModBase	Search...
Genome annotation databases
GeneID	2737464.
KEGG	bba:Bd3755.
NMPDR	fig|264462.1.peg.3436.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	EGHAIMA.
PhylomeDB	Q6MH10.
Enzyme and pathway databases
BioCyc	BBAC264462:BD3755-MONOMER.
BRENDA	3.4.11.1. 3459.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BDEBA

Accession

Primary (citable) accession number: Q6MH10

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)