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Protein

E3 ubiquitin-protein ligase RING1

Gene

Ring1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri48 – 8841RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-2559580. Oxidative Stress Induced Senescence.
R-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
R-RNO-4570464. SUMOylation of RNA binding proteins.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RING1 (EC:6.3.2.-)
Alternative name(s):
Polycomb complex protein RING1
RING finger protein 1
Gene namesi
Name:Ring1
Synonyms:Rnf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3576. Ring1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406E3 ubiquitin-protein ligase RING1PRO_0000056387Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241PhosphothreonineBy similarity
Modified residuei38 – 381PhosphoserineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei187 – 1871PhosphoserineBy similarity
Modified residuei190 – 1901PhosphoserineBy similarity
Modified residuei215 – 2151PhosphothreonineBy similarity
Modified residuei229 – 2291PhosphoserineBy similarity
Modified residuei232 – 2321PhosphoserineBy similarity
Modified residuei248 – 2481PhosphoserineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6MGB6.

Expressioni

Gene expression databases

ExpressionAtlasiQ6MGB6. baseline and differential.
GenevisibleiQ6MGB6. RN.

Interactioni

Subunit structurei

Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of a PRC1-like complex. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2. Interacts with BMI1, PHC2, PCGF2, RNF2; CBX6, CBX7 and CBX8 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000543.

Structurei

3D structure databases

ProteinModelPortaliQ6MGB6.
SMRiQ6MGB6. Positions 12-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 234205Necessary for transcriptional repressionBy similarityAdd
BLAST
Regioni230 – 406177Necessary for interaction with CBX2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 2044Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi171 – 344174Gly-richAdd
BLAST
Compositional biasi246 – 25813Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri48 – 8841RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
HOGENOMiHOG000273917.
HOVERGENiHBG079942.
InParanoidiQ6MGB6.
KOiK10695.
OMAiQSASKTW.
OrthoDBiEOG7NGQC3.
PhylomeDBiQ6MGB6.
TreeFamiTF105501.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6MGB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPANAQNA SKTWELSLYE LHRTPQEAIM DGTEIAVSPR SLHSELMCPI
60 70 80 90 100
CLDMLKNTMT TKECLHRFCS DCIVTALRSG NKECPTCRKK LVSKRSLRPD
110 120 130 140 150
PNFDALISKI YPSREEYEAH QDRVLIRLSR LHNQQALSSS IEEGLRMQAM
160 170 180 190 200
HRAQRVRRPM PGSDQTTTMS GGEGEPGEGE GDGEDISSDS APDSAPGPAP
210 220 230 240 250
KRPRGGGAGG SSVGTGGGAA GGACGGAGSE DSGDRGGTLG GGTLGPPSPP
260 270 280 290 300
GAPSPPEPGG EIELVFRPHP LLVEKGEYCQ TRYVKTTGNA TVDHLSKYLA
310 320 330 340 350
LRIALERRQQ QETVEPGGPG GGASDTGGPD GGGGERGVSG GGEGPEEPAL
360 370 380 390 400
PSLEGVSEKQ YTIYIAPGGG AFTTLNGSLT LELVNEKFWK VSRPLELCYA

PTKDPK
Length:406
Mass (Da):42,661
Last modified:March 21, 2006 - v2
Checksum:i61CC002752031C9F
GO
Isoform 2 (identifier: Q6MGB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Show »
Length:377
Mass (Da):39,377
Checksum:iFC939166B83D4E5D
GO

Sequence cautioni

The sequence AAH98635.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → C in CAA64746 (PubMed:8662089).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_017698Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83930.1.
BC098635 mRNA. Translation: AAH98635.2. Different initiation.
X95474 Genomic DNA. Translation: CAA64746.1.
RefSeqiNP_997714.2. NM_212549.2. [Q6MGB6-1]
UniGeneiRn.116589.

Genome annotation databases

EnsembliENSRNOT00000000543; ENSRNOP00000000543; ENSRNOG00000000467. [Q6MGB6-1]
GeneIDi309626.
KEGGirno:309626.
UCSCiRGD:3576. rat. [Q6MGB6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83930.1.
BC098635 mRNA. Translation: AAH98635.2. Different initiation.
X95474 Genomic DNA. Translation: CAA64746.1.
RefSeqiNP_997714.2. NM_212549.2. [Q6MGB6-1]
UniGeneiRn.116589.

3D structure databases

ProteinModelPortaliQ6MGB6.
SMRiQ6MGB6. Positions 12-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000543.

Proteomic databases

PaxDbiQ6MGB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000543; ENSRNOP00000000543; ENSRNOG00000000467. [Q6MGB6-1]
GeneIDi309626.
KEGGirno:309626.
UCSCiRGD:3576. rat. [Q6MGB6-1]

Organism-specific databases

CTDi6015.
RGDi3576. Ring1.

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
HOGENOMiHOG000273917.
HOVERGENiHBG079942.
InParanoidiQ6MGB6.
KOiK10695.
OMAiQSASKTW.
OrthoDBiEOG7NGQC3.
PhylomeDBiQ6MGB6.
TreeFamiTF105501.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-2559580. Oxidative Stress Induced Senescence.
R-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
R-RNO-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

NextBioi661076.
PROiQ6MGB6.

Gene expression databases

ExpressionAtlasiQ6MGB6. baseline and differential.
GenevisibleiQ6MGB6. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Brown Norway.
    Tissue: Lung.
  3. "Physical mapping of the Ring1, Ring2, Ke6, Ke4, Rxrb, Col11a2 and RT1.Hb genes of the rat major histocompatibility complex."
    Walter L., Fischer K., Guenther E.
    Immunogenetics 44:218-221(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-78.
    Strain: Lewis.

Entry informationi

Entry nameiRING1_RAT
AccessioniPrimary (citable) accession number: Q6MGB6
Secondary accession number(s): Q4KMC1, Q63510
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 21, 2006
Last modified: May 11, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.