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Protein

E3 ubiquitin-protein ligase RING1

Gene

Ring1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity (By similarity).By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri48 – 88RING-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor, Transferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-2559580. Oxidative Stress Induced Senescence.
R-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
R-RNO-4551638. SUMOylation of chromatin organization proteins.
R-RNO-8939243. RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known.
R-RNO-8943724. Regulation of PTEN gene transcription.
R-RNO-8953750. Transcriptional Regulation by E2F6.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RING1 (EC:2.3.2.27)
Alternative name(s):
Polycomb complex protein RING1
RING finger protein 1
RING-type E3 ubiquitin transferase RING1Curated
Gene namesi
Name:Ring1
Synonyms:Rnf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3576. Ring1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563871 – 406E3 ubiquitin-protein ligase RING1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphothreonineBy similarity1
Modified residuei38PhosphoserineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei187PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei215PhosphothreonineBy similarity1
Modified residuei229PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1
Modified residuei254PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6MGB6.
PRIDEiQ6MGB6.

PTM databases

iPTMnetiQ6MGB6.
PhosphoSitePlusiQ6MGB6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000467.
ExpressionAtlasiQ6MGB6. baseline and differential.
GenevisibleiQ6MGB6. RN.

Interactioni

Subunit structurei

Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of a PRC1-like complex. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2. Interacts with BMI1, PHC2, PCGF2, RNF2; CBX6, CBX7 and CBX8 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000543.

Structurei

3D structure databases

ProteinModelPortaliQ6MGB6.
SMRiQ6MGB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 234Necessary for transcriptional repressionBy similarityAdd BLAST205
Regioni230 – 406Necessary for interaction with CBX2By similarityAdd BLAST177

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 204Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi171 – 344Gly-richAdd BLAST174
Compositional biasi246 – 258Pro-richAdd BLAST13

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri48 – 88RING-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
HOGENOMiHOG000273917.
HOVERGENiHBG079942.
InParanoidiQ6MGB6.
KOiK10695.
OMAiQSASKTW.
OrthoDBiEOG091G06VO.
PhylomeDBiQ6MGB6.
TreeFamiTF105501.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF16207. RAWUL. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6MGB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPANAQNA SKTWELSLYE LHRTPQEAIM DGTEIAVSPR SLHSELMCPI
60 70 80 90 100
CLDMLKNTMT TKECLHRFCS DCIVTALRSG NKECPTCRKK LVSKRSLRPD
110 120 130 140 150
PNFDALISKI YPSREEYEAH QDRVLIRLSR LHNQQALSSS IEEGLRMQAM
160 170 180 190 200
HRAQRVRRPM PGSDQTTTMS GGEGEPGEGE GDGEDISSDS APDSAPGPAP
210 220 230 240 250
KRPRGGGAGG SSVGTGGGAA GGACGGAGSE DSGDRGGTLG GGTLGPPSPP
260 270 280 290 300
GAPSPPEPGG EIELVFRPHP LLVEKGEYCQ TRYVKTTGNA TVDHLSKYLA
310 320 330 340 350
LRIALERRQQ QETVEPGGPG GGASDTGGPD GGGGERGVSG GGEGPEEPAL
360 370 380 390 400
PSLEGVSEKQ YTIYIAPGGG AFTTLNGSLT LELVNEKFWK VSRPLELCYA

PTKDPK
Length:406
Mass (Da):42,661
Last modified:March 21, 2006 - v2
Checksum:i61CC002752031C9F
GO
Isoform 2 (identifier: Q6MGB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Show »
Length:377
Mass (Da):39,377
Checksum:iFC939166B83D4E5D
GO

Sequence cautioni

The sequence AAH98635 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65L → C in CAA64746 (PubMed:8662089).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0176981 – 29Missing in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83930.1.
BC098635 mRNA. Translation: AAH98635.2. Different initiation.
X95474 Genomic DNA. Translation: CAA64746.1.
RefSeqiNP_997714.2. NM_212549.2. [Q6MGB6-1]
UniGeneiRn.116589.

Genome annotation databases

EnsembliENSRNOT00000000543; ENSRNOP00000000543; ENSRNOG00000000467. [Q6MGB6-1]
GeneIDi309626.
KEGGirno:309626.
UCSCiRGD:3576. rat. [Q6MGB6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRING1_RAT
AccessioniPrimary (citable) accession number: Q6MGB6
Secondary accession number(s): Q4KMC1, Q63510
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 21, 2006
Last modified: November 22, 2017
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways