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Protein

Estradiol 17-beta-dehydrogenase 8

Gene

Hsd17b8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (By similarity). The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria (By similarity).By similarity

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Testosterone + NAD+ = androstenedione + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Pathwayi: estrogen biosynthesis

This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei154 – 1541SubstrateBy similarity
Active sitei167 – 1671Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 219NADBy similarity
Nucleotide bindingi40 – 412NADBy similarity
Nucleotide bindingi72 – 743NADBy similarity
Nucleotide bindingi167 – 1715NADBy similarity
Nucleotide bindingi200 – 2023NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00094.
UPA00769.

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17-beta-dehydrogenase 8 (EC:1.1.1.62)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 8
Short name:
17-beta-HSD 8
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-)
Testosterone 17-beta-dehydrogenase 8 (EC:1.1.1.239)
Gene namesi
Name:Hsd17b8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1303158. Hsd17b8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Estradiol 17-beta-dehydrogenase 8PRO_0000304877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581PhosphoserineCombined sources
Modified residuei66 – 661N6-acetyllysineBy similarity
Modified residuei158 – 1581N6-succinyllysineBy similarity
Modified residuei171 – 1711N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6MGB5.
PRIDEiQ6MGB5.

PTM databases

PhosphoSiteiQ6MGB5.

Expressioni

Gene expression databases

ExpressionAtlasiQ6MGB5. baseline and differential.
GenevisibleiQ6MGB5. RN.

Interactioni

Subunit structurei

Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.By similarity

Protein-protein interaction databases

IntActiQ6MGB5. 1 interaction.
STRINGi10116.ENSRNOP00000000542.

Structurei

3D structure databases

ProteinModelPortaliQ6MGB5.
SMRiQ6MGB5. Positions 7-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ6MGB5.
KOiK13370.
OMAiLPAMIEQ.
OrthoDBiEOG73Z2VD.
PhylomeDBiQ6MGB5.
TreeFamiTF313099.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MGB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQLRLRSA LALVTGAGSG IGRAISVRLA AEGAAVAACD LDGAAAQDTV
60 70 80 90 100
RLLGNPGSED REPRGKHAAF QADVSEGPAA KRLLEQVQAC FFRPPSVVVS
110 120 130 140 150
CAGITRDEFL LHMSEEDWDR VIAVNLKGTF LVTQAAAQAL VSSGGRGSII
160 170 180 190 200
NISSIVGKVG NIGQTNYASS KAGVIGLTQT AARELGRHGI RCNSVLPGFI
210 220 230 240 250
ATPMTQKMPE KVKDKVTAMI PLGHMGDPED VADVVAFLAS EDSGYITGAS

VEVSGGLFM
Length:259
Mass (Da):26,791
Last modified:July 5, 2004 - v1
Checksum:i5E23ECCE525E6781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83931.1.
RefSeqiNP_997694.1. NM_212529.1.
UniGeneiRn.137469.

Genome annotation databases

EnsembliENSRNOT00000000542; ENSRNOP00000000542; ENSRNOG00000000466.
GeneIDi361802.
KEGGirno:361802.
UCSCiRGD:1303158. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83931.1.
RefSeqiNP_997694.1. NM_212529.1.
UniGeneiRn.137469.

3D structure databases

ProteinModelPortaliQ6MGB5.
SMRiQ6MGB5. Positions 7-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6MGB5. 1 interaction.
STRINGi10116.ENSRNOP00000000542.

PTM databases

PhosphoSiteiQ6MGB5.

Proteomic databases

PaxDbiQ6MGB5.
PRIDEiQ6MGB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000542; ENSRNOP00000000542; ENSRNOG00000000466.
GeneIDi361802.
KEGGirno:361802.
UCSCiRGD:1303158. rat.

Organism-specific databases

CTDi7923.
RGDi1303158. Hsd17b8.

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ6MGB5.
KOiK13370.
OMAiLPAMIEQ.
OrthoDBiEOG73Z2VD.
PhylomeDBiQ6MGB5.
TreeFamiTF313099.

Enzyme and pathway databases

UniPathwayiUPA00094.
UPA00769.

Miscellaneous databases

NextBioi677656.
PROiQ6MGB5.

Gene expression databases

ExpressionAtlasiQ6MGB5. baseline and differential.
GenevisibleiQ6MGB5. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHB8_RAT
AccessioniPrimary (citable) accession number: Q6MGB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.