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Protein

Estradiol 17-beta-dehydrogenase 8

Gene

Hsd17b8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis. Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD.By similarity

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.By similarity
Testosterone + NAD+ = androstenedione + NADH.By similarity

Pathwayi: estrogen biosynthesis

This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154SubstrateBy similarity1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 21NADBy similarity9
Nucleotide bindingi40 – 41NADBy similarity2
Nucleotide bindingi72 – 74NADBy similarity3
Nucleotide bindingi167 – 171NADBy similarity5
Nucleotide bindingi200 – 202NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-RNO-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
UniPathwayiUPA00094.
UPA00769.

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17-beta-dehydrogenase 8 (EC:1.1.1.62)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 8
Short name:
17-beta-HSD 8
3-ketoacyl-[acyl-carrier-protein] reductase alpha subunitBy similarity
Short name:
KAR alpha subunitBy similarity
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-By similarity)
Testosterone 17-beta-dehydrogenase 8 (EC:1.1.1.239)
Gene namesi
Name:Hsd17b8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1303158. Hsd17b8.

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003048771 – 259Estradiol 17-beta-dehydrogenase 8Add BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei66N6-acetyllysineBy similarity1
Modified residuei158N6-succinyllysineBy similarity1
Modified residuei171N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6MGB5.
PRIDEiQ6MGB5.

PTM databases

iPTMnetiQ6MGB5.
PhosphoSitePlusiQ6MGB5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000466.
ExpressionAtlasiQ6MGB5. baseline and differential.
GenevisibleiQ6MGB5. RN.

Interactioni

Subunit structurei

Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.By similarity

Protein-protein interaction databases

IntActiQ6MGB5. 1 interactor.
STRINGi10116.ENSRNOP00000000542.

Structurei

3D structure databases

ProteinModelPortaliQ6MGB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ6MGB5.
KOiK13370.
PhylomeDBiQ6MGB5.
TreeFamiTF313099.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MGB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQLRLRSA LALVTGAGSG IGRAISVRLA AEGAAVAACD LDGAAAQDTV
60 70 80 90 100
RLLGNPGSED REPRGKHAAF QADVSEGPAA KRLLEQVQAC FFRPPSVVVS
110 120 130 140 150
CAGITRDEFL LHMSEEDWDR VIAVNLKGTF LVTQAAAQAL VSSGGRGSII
160 170 180 190 200
NISSIVGKVG NIGQTNYASS KAGVIGLTQT AARELGRHGI RCNSVLPGFI
210 220 230 240 250
ATPMTQKMPE KVKDKVTAMI PLGHMGDPED VADVVAFLAS EDSGYITGAS

VEVSGGLFM
Length:259
Mass (Da):26,791
Last modified:July 5, 2004 - v1
Checksum:i5E23ECCE525E6781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83931.1.
RefSeqiNP_997694.1. NM_212529.1.
UniGeneiRn.137469.

Genome annotation databases

EnsembliENSRNOT00000000542; ENSRNOP00000000542; ENSRNOG00000000466.
GeneIDi361802.
KEGGirno:361802.
UCSCiRGD:1303158. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83931.1.
RefSeqiNP_997694.1. NM_212529.1.
UniGeneiRn.137469.

3D structure databases

ProteinModelPortaliQ6MGB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6MGB5. 1 interactor.
STRINGi10116.ENSRNOP00000000542.

PTM databases

iPTMnetiQ6MGB5.
PhosphoSitePlusiQ6MGB5.

Proteomic databases

PaxDbiQ6MGB5.
PRIDEiQ6MGB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000542; ENSRNOP00000000542; ENSRNOG00000000466.
GeneIDi361802.
KEGGirno:361802.
UCSCiRGD:1303158. rat.

Organism-specific databases

CTDi7923.
RGDi1303158. Hsd17b8.

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ6MGB5.
KOiK13370.
PhylomeDBiQ6MGB5.
TreeFamiTF313099.

Enzyme and pathway databases

UniPathwayiUPA00094.
UPA00769.
ReactomeiR-RNO-535734. Fatty acid, triacylglycerol, and ketone body metabolism.

Miscellaneous databases

PROiQ6MGB5.

Gene expression databases

BgeeiENSRNOG00000000466.
ExpressionAtlasiQ6MGB5. baseline and differential.
GenevisibleiQ6MGB5. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHB8_RAT
AccessioniPrimary (citable) accession number: Q6MGB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.