Reviewed, UniProtKB/Swiss-Prot Q6MGB5 (DHB8_RAT)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 8
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase 8
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 8
      Short name=17-beta-HSD 8

Gene names

Name:

Hsd17b8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Uses estradiol as its preferred substrate.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Estradiol 17-beta-dehydrogenase 8		PRO_0000304877
Regions
	Nucleotide binding	13 – 37	25	NAD By similarity
Sites
	Active site	167	1	Proton acceptor By similarity
	Binding site	154	1	Substrate By similarity
Amino acid modifications
	Modified residue	66	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MGB5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5E23ECCE525E6781	FASTA	259	26,791
	10 20 30 40 50 60 MASQLRLRSA LALVTGAGSG IGRAISVRLA AEGAAVAACD LDGAAAQDTV RLLGNPGSED 70 80 90 100 110 120 REPRGKHAAF QADVSEGPAA KRLLEQVQAC FFRPPSVVVS CAGITRDEFL LHMSEEDWDR 130 140 150 160 170 180 VIAVNLKGTF LVTQAAAQAL VSSGGRGSII NISSIVGKVG NIGQTNYASS KAGVIGLTQT 190 200 210 220 230 240 AARELGRHGI RCNSVLPGFI ATPMTQKMPE KVKDKVTAMI PLGHMGDPED VADVVAFLAS 250 EDSGYITGAS VEVSGGLFM

References

[1]

"The genomic sequence and comparative analysis of the rat major histocompatibility complex." Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H. Genome Res. 14:631-639(2004) [PubMed: 15060004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX883042 Genomic DNA. Translation: CAE83931.1.
RefSeq	NP_997694.1.
UniGene	Rn.137469
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000000466. Rattus norvegicus. [Contig view]
GeneID	361802.
KEGG	rno:361802.
NMPDR	fig|10116.3.peg.17523.
Organism-specific databases
RGD	1303158. Hsd17b8.
Phylogenomic databases
HOVERGEN	Q6MGB5.
Gene expression databases
ArrayExpress	Q6MGB5.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	677656.

Entry information

Entry name

DHB8_RAT

Accession

Primary (citable) accession number: Q6MGB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)