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Protein

Bromodomain-containing protein 2

Gene

Brd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly (By similarity). May play a role in spermatogenesis or folliculogenesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 2
Alternative name(s):
Protein RING3
Gene namesi
Name:Brd2
Synonyms:Ring3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1303324. Brd2.

Subcellular locationi

  • Nucleus By similarity

  • Note: Detected on chromatin and nucleosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Bromodomain-containing protein 2PRO_0000274006Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei6 – 61PhosphothreonineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineBy similarity
Modified residuei304 – 3041PhosphoserineBy similarity
Modified residuei631 – 6311PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6MGA9.
PRIDEiQ6MGA9.

PTM databases

iPTMnetiQ6MGA9.
PhosphoSiteiQ6MGA9.

Expressioni

Gene expression databases

ExpressionAtlasiQ6MGA9. baseline.
GenevisibleiQ6MGA9. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with E2F1 and with histone H4 acetylated at 'Lys-13' (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000056940.

Structurei

3D structure databases

ProteinModelPortaliQ6MGA9.
SMRiQ6MGA9. Positions 72-185, 347-454, 635-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 16273Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini363 – 43573Bromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini630 – 71283NETPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi553 – 5575Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 634Poly-Pro
Compositional biasi228 – 331104Pro-richAdd
BLAST
Compositional biasi478 – 52346Glu-richAdd
BLAST
Compositional biasi549 – 5579Poly-Lys
Compositional biasi589 – 5924Poly-Gly
Compositional biasi632 – 6365Poly-Glu
Compositional biasi761 – 79737Ser-richAdd
BLAST

Domaini

One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.By similarity

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ6MGA9.
KOiK08871.
OMAiTESSVAQ.
OrthoDBiEOG7TTQ86.
PhylomeDBiQ6MGA9.
TreeFamiTF317345.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MGA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQNVTPHKL PGEGNAGLLG LGPEAAAPGK RIRKPSLLYE GFESPTMASV
60 70 80 90 100
PALQLAPANP PPPEVSNPKK PGRVTNQLQY LHKVVMKALW KHQFAWPFRQ
110 120 130 140 150
PVDAVKLGLP DYHKIIKQPM DMGTIKRRLE NNYYWAASEC MQDFNTMFTN
160 170 180 190 200
CYIYNKPTDD IVLMAQTLEK IFLQKVASMP QEEQELVVTI PKNSHKKGAK
210 220 230 240 250
LAALQGSITS AHQVPAVSSV SHTALYTPPP EIPTTVLNIP HPSVISSPLL
260 270 280 290 300
KSLHSAGPPL LAVSAAPPAQ PLAKKKGVKR KADTTTPTPT AILAPGSPAS
310 320 330 340 350
PPGSLEPKAA RLPPMRRESG RPIKPPRKDL PDSQQQHQSS KKGKLSEQLK
360 370 380 390 400
HCNGILKELL SKKHAAYAWP FYKPVDASAL GLHDYHDIIK HPMDLSTVKR
410 420 430 440 450
KMENRDYRDA QEFAADVRLM FSNCYKYNPP DHDVVAMARK LQDVFEFRYA
460 470 480 490 500
KMPDEPLEPG PLPVSTALPP GLAKSSSESS SEESSSESSS EEEEEEDEED
510 520 530 540 550
EEEESESSDS EEERAHRLAE LQEQLRAVHE QLAALSQGPI SKPKRKREKK
560 570 580 590 600
EKKKKRKAEK HRGRIGIDED DKGPRAPRPL QPKKSKKAGG GGSNATTLSH
610 620 630 640 650
PGFGTSAGSS NKLPKKAQKT APPVLPTGYD SEEEEESRPM SYDEKRQLSL
660 670 680 690 700
DINKLPGEKL GRVVHIIQAR EPSLRDSNPE EIEIDFETLK PSTLRELERY
710 720 730 740 750
VLSCLRKKPR KPYTIRKPVG KTKEELALEK KRELEKRLQD VSGQLNSTKK
760 770 780 790
PPKKASEKTE SSAQQVAVSR LSASSSSSDS SSSSSSSSSS DTSDSDSG
Length:798
Mass (Da):88,051
Last modified:July 5, 2004 - v1
Checksum:iA6A530A04AB12EC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83937.1.
RefSeqiNP_997660.1. NM_212495.1.
XP_006256099.1. XM_006256037.2.
UniGeneiRn.98146.

Genome annotation databases

EnsembliENSRNOT00000000535; ENSRNOP00000000535; ENSRNOG00000000461.
GeneIDi100909544.
294276.
KEGGirno:100909544.
rno:294276.
UCSCiRGD:1303324. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883042 Genomic DNA. Translation: CAE83937.1.
RefSeqiNP_997660.1. NM_212495.1.
XP_006256099.1. XM_006256037.2.
UniGeneiRn.98146.

3D structure databases

ProteinModelPortaliQ6MGA9.
SMRiQ6MGA9. Positions 72-185, 347-454, 635-712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000056940.

PTM databases

iPTMnetiQ6MGA9.
PhosphoSiteiQ6MGA9.

Proteomic databases

PaxDbiQ6MGA9.
PRIDEiQ6MGA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000535; ENSRNOP00000000535; ENSRNOG00000000461.
GeneIDi100909544.
294276.
KEGGirno:100909544.
rno:294276.
UCSCiRGD:1303324. rat.

Organism-specific databases

CTDi6046.
RGDi1303324. Brd2.

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ6MGA9.
KOiK08871.
OMAiTESSVAQ.
OrthoDBiEOG7TTQ86.
PhylomeDBiQ6MGA9.
TreeFamiTF317345.

Miscellaneous databases

PROiQ6MGA9.

Gene expression databases

ExpressionAtlasiQ6MGA9. baseline.
GenevisibleiQ6MGA9. RN.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297 AND SER-631, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBRD2_RAT
AccessioniPrimary (citable) accession number: Q6MGA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.