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Protein

Decapping and exoribonuclease protein

Gene

Dxo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei101SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Metal bindingi192Magnesium 1By similarity1
Metal bindingi192Magnesium 2By similarity1
Binding sitei217SubstrateBy similarity1
Metal bindingi234Magnesium 2By similarity1
Binding sitei234SubstrateBy similarity1
Metal bindingi236Magnesium 1By similarity1
Metal bindingi236Magnesium 2By similarity1
Metal bindingi253Magnesium 1By similarity1
Metal bindingi254Magnesium 1; via carbonyl oxygenBy similarity1
Binding sitei280SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Decapping and exoribonuclease protein (EC:3.1.13.-, EC:3.6.1.-)
Short name:
DXO
Alternative name(s):
Dom-3 homolog Z
Gene namesi
Name:Dxo
Synonyms:Dom3z
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1303267. Dxo.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498241 – 397Decapping and exoribonuclease proteinAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei392PhosphothreonineCombined sources1
Modified residuei394PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6MG77.

PTM databases

iPTMnetiQ6MG77.
PhosphoSitePlusiQ6MG77.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000422.
GenevisibleiQ6MG77. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000483.

Family & Domainsi

Sequence similaritiesi

Belongs to the DXO/Dom3Z family.Curated

Phylogenomic databases

eggNOGiKOG1982. Eukaryota.
ENOG410ZAFR. LUCA.
GeneTreeiENSGT00390000006425.
HOGENOMiHOG000200647.
HOVERGENiHBG080627.
InParanoidiQ6MG77.
KOiK14845.
OMAiHSPGQWR.
OrthoDBiEOG091G0F0X.
PhylomeDBiQ6MG77.
TreeFamiTF322812.

Family and domain databases

InterProiIPR013961. RAI1.
[Graphical view]
PfamiPF08652. RAI1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MG77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRGTKRKA EKTEVAKTWN KLPRSLPSLR TQPSLYSGPF PFYRRPSELG
60 70 80 90 100
CFSLDAQRQY HGDARALRYY SPPPINGPGP DFDLRDGYPD RYQPRDEEVR
110 120 130 140 150
ERLDHLLRWV LEHRNQLEGG PGWLAGATVT WRGHLTKLLT TPYERQEGWQ
160 170 180 190 200
LAASRFQGTL YLSEVETPAA RAQRLARPPL LRELMYMGYK FEQYMCADKP
210 220 230 240 250
GGSPDPSGEV NTNVAFCSVL RSRLGNHPLL FSGEVDCLNP QAPCTQPPSC
260 270 280 290 300
YVELKTSKEM HSPGQWRSFY RHKLLKWWAQ SFLPGVPYVV AGFRNPEGFV
310 320 330 340 350
CSLKTFPTME MFENVRNDQE GWNPSVCMNF CAAFLSFAQS TVVQDDPRLV
360 370 380 390
HLFSWEPGGP VTVSVHRDAP YAFLPSWYVE AMTQDLPSLS KTPSPKD
Length:397
Mass (Da):45,299
Last modified:July 5, 2004 - v1
Checksum:i29572F58C6138ACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83969.1.
BC082083 mRNA. Translation: AAH82083.1.
RefSeqiNP_997662.1. NM_212497.2.
UniGeneiRn.20463.

Genome annotation databases

EnsembliENSRNOT00000000483; ENSRNOP00000000483; ENSRNOG00000000422.
GeneIDi361799.
KEGGirno:361799.
UCSCiRGD:1303267. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83969.1.
BC082083 mRNA. Translation: AAH82083.1.
RefSeqiNP_997662.1. NM_212497.2.
UniGeneiRn.20463.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000483.

PTM databases

iPTMnetiQ6MG77.
PhosphoSitePlusiQ6MG77.

Proteomic databases

PaxDbiQ6MG77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000483; ENSRNOP00000000483; ENSRNOG00000000422.
GeneIDi361799.
KEGGirno:361799.
UCSCiRGD:1303267. rat.

Organism-specific databases

CTDi1797.
RGDi1303267. Dxo.

Phylogenomic databases

eggNOGiKOG1982. Eukaryota.
ENOG410ZAFR. LUCA.
GeneTreeiENSGT00390000006425.
HOGENOMiHOG000200647.
HOVERGENiHBG080627.
InParanoidiQ6MG77.
KOiK14845.
OMAiHSPGQWR.
OrthoDBiEOG091G0F0X.
PhylomeDBiQ6MG77.
TreeFamiTF322812.

Miscellaneous databases

PROiQ6MG77.

Gene expression databases

BgeeiENSRNOG00000000422.
GenevisibleiQ6MG77. RN.

Family and domain databases

InterProiIPR013961. RAI1.
[Graphical view]
PfamiPF08652. RAI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDXO_RAT
AccessioniPrimary (citable) accession number: Q6MG77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.