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Protein

Chloride intracellular channel protein 1

Gene

Clic1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions (By similarity).By similarity

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 1By similarityImported
Gene namesi
Name:Clic1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1303043. Clic1.

Subcellular locationi

  • Nucleus By similarity
  • Nucleus membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity; Single-pass membrane protein By similarity

  • Note: Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei26 – 46Helical; Note=After insertion into the membraneSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003651032 – 241Chloride intracellular channel protein 1By similarityAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei13N6-acetyllysineBy similarity1
Disulfide bondi24 ↔ 59By similarity
Modified residuei119N6-acetyllysineBy similarity1
Modified residuei121PhosphoserineBy similarity1
Modified residuei131N6-acetyllysineBy similarity1
Modified residuei156PhosphoserineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Modified residuei233PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ6MG61.
PRIDEiQ6MG61.

PTM databases

iPTMnetiQ6MG61.
PhosphoSitePlusiQ6MG61.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029682.
GenevisibleiQ6MG61. RN.

Interactioni

Subunit structurei

Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060240.

Structurei

3D structure databases

ProteinModelPortaliQ6MG61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 233GST C-terminalSequence analysisAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 90Required for insertion into the membraneBy similarityAdd BLAST89

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Sequence analysis
Contains 1 GST C-terminal domain.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INGZ. Eukaryota.
ENOG410ZZZX. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ6MG61.
KOiK05021.
OMAiVCLKYRS.
OrthoDBiEOG091G0IHT.
PhylomeDBiQ6MG61.
TreeFamiTF315438.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030259. CLIC-1.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF210. PTHR11260:SF210. 2 hits.
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6MG61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR
60 70 80 90 100
RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN
110 120 130 140 150
PESNTSGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE
160 170 180 190 200
EVDETSAEDE GISQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP
210 220 230 240
EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVARAL K
Length:241
Mass (Da):26,981
Last modified:July 5, 2004 - v1
Checksum:i1774F7190D44B01C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83985.1.
CH474121 Genomic DNA. Translation: EDL83493.1.
BC099823 mRNA. Translation: AAH99823.1.
RefSeqiNP_001002807.1. NM_001002807.2.
UniGeneiRn.203139.

Genome annotation databases

EnsembliENSRNOT00000068435; ENSRNOP00000060240; ENSRNOG00000029682.
GeneIDi406864.
KEGGirno:406864.
UCSCiRGD:1303043. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83985.1.
CH474121 Genomic DNA. Translation: EDL83493.1.
BC099823 mRNA. Translation: AAH99823.1.
RefSeqiNP_001002807.1. NM_001002807.2.
UniGeneiRn.203139.

3D structure databases

ProteinModelPortaliQ6MG61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060240.

PTM databases

iPTMnetiQ6MG61.
PhosphoSitePlusiQ6MG61.

Proteomic databases

PaxDbiQ6MG61.
PRIDEiQ6MG61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068435; ENSRNOP00000060240; ENSRNOG00000029682.
GeneIDi406864.
KEGGirno:406864.
UCSCiRGD:1303043. rat.

Organism-specific databases

CTDi1192.
RGDi1303043. Clic1.

Phylogenomic databases

eggNOGiENOG410INGZ. Eukaryota.
ENOG410ZZZX. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ6MG61.
KOiK05021.
OMAiVCLKYRS.
OrthoDBiEOG091G0IHT.
PhylomeDBiQ6MG61.
TreeFamiTF315438.

Miscellaneous databases

PROiQ6MG61.

Gene expression databases

BgeeiENSRNOG00000029682.
GenevisibleiQ6MG61. RN.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030259. CLIC-1.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF210. PTHR11260:SF210. 2 hits.
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLIC1_RAT
AccessioniPrimary (citable) accession number: Q6MG61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.