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Q6MG60

- DDAH2_RAT

UniProt

Q6MG60 - DDAH2_RAT

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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

Ddah2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation (By similarity).By similarity

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Proton donorBy similarity
Active sitei276 – 2761NucleophileSequence Analysis

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. dimethylargininase activity Source: UniProtKB

GO - Biological processi

  1. arginine metabolic process Source: RGD
  2. citrulline metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene namesi
Name:Ddah2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi1302955. Ddah2.

Subcellular locationi

Cytoplasm By similarity. Mitochondrion By similarity
Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2PRO_0000270760Add
BLAST

Proteomic databases

PaxDbiQ6MG60.
PRIDEiQ6MG60.

Expressioni

Gene expression databases

GenevestigatoriQ6MG60.

Interactioni

Protein-protein interaction databases

BioGridi254630. 1 interaction.
IntActiQ6MG60. 1 interaction.
STRINGi10116.ENSRNOP00000001118.

Structurei

3D structure databases

ProteinModelPortaliQ6MG60.
SMRiQ6MG60. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiNOG86337.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ6MG60.
KOiK01482.
OMAiALPFLLH.
OrthoDBiEOG73V6KV.
PhylomeDBiQ6MG60.
TreeFamiTF314737.

Family and domain databases

InterProiIPR003198. Amidino_trans.
[Graphical view]
PfamiPF02274. Amidinotransf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MG60-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGAS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA
210 220 230 240 250
ALTDHPYASL TLPDDAASDC LFLRPGLPGT TPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKVGA GLSSLCLVLS TRPHC
Length:285
Mass (Da):29,688
Last modified:July 5, 2004 - v1
Checksum:iC28D60FF20CEAEFF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX883045 Genomic DNA. Translation: CAE83986.1.
BC086443 mRNA. Translation: AAH86443.1.
BC097930 mRNA. Translation: AAH97930.1.
RefSeqiNP_001159408.1. NM_001165936.1.
NP_997697.1. NM_212532.2.
UniGeneiRn.203056.

Genome annotation databases

EnsembliENSRNOT00000001118; ENSRNOP00000001118; ENSRNOG00000000842.
GeneIDi294239.
KEGGirno:294239.
UCSCiRGD:1302955. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX883045 Genomic DNA. Translation: CAE83986.1 .
BC086443 mRNA. Translation: AAH86443.1 .
BC097930 mRNA. Translation: AAH97930.1 .
RefSeqi NP_001159408.1. NM_001165936.1.
NP_997697.1. NM_212532.2.
UniGenei Rn.203056.

3D structure databases

ProteinModelPortali Q6MG60.
SMRi Q6MG60. Positions 8-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 254630. 1 interaction.
IntActi Q6MG60. 1 interaction.
STRINGi 10116.ENSRNOP00000001118.

Chemistry

BindingDBi Q6MG60.

Proteomic databases

PaxDbi Q6MG60.
PRIDEi Q6MG60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001118 ; ENSRNOP00000001118 ; ENSRNOG00000000842 .
GeneIDi 294239.
KEGGi rno:294239.
UCSCi RGD:1302955. rat.

Organism-specific databases

CTDi 23564.
RGDi 1302955. Ddah2.

Phylogenomic databases

eggNOGi NOG86337.
GeneTreei ENSGT00390000009331.
HOGENOMi HOG000161035.
HOVERGENi HBG055937.
InParanoidi Q6MG60.
KOi K01482.
OMAi ALPFLLH.
OrthoDBi EOG73V6KV.
PhylomeDBi Q6MG60.
TreeFami TF314737.

Miscellaneous databases

NextBioi 637810.
PROi Q6MG60.

Gene expression databases

Genevestigatori Q6MG60.

Family and domain databases

InterProi IPR003198. Amidino_trans.
[Graphical view ]
Pfami PF02274. Amidinotransf. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Thymus.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 106-142; 148-173; 183-194 AND 268-285, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.

Entry informationi

Entry nameiDDAH2_RAT
AccessioniPrimary (citable) accession number: Q6MG60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3