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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

Ddah2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation (By similarity).By similarity

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Proton donorBy similarity
Active sitei276 – 2761NucleophileSequence Analysis

GO - Molecular functioni

  • amino acid binding Source: RGD
  • dimethylargininase activity Source: UniProtKB

GO - Biological processi

  • arginine metabolic process Source: RGD
  • citrulline metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.3.18. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene namesi
Name:Ddah2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1302955. Ddah2.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity

  • Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2PRO_0000270760Add
BLAST

Proteomic databases

PaxDbiQ6MG60.
PRIDEiQ6MG60.

Expressioni

Gene expression databases

GenevestigatoriQ6MG60.

Interactioni

Protein-protein interaction databases

BioGridi254630. 1 interaction.
IntActiQ6MG60. 1 interaction.
STRINGi10116.ENSRNOP00000001118.

Structurei

3D structure databases

ProteinModelPortaliQ6MG60.
SMRiQ6MG60. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiNOG86337.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ6MG60.
KOiK01482.
OMAiMANVCPY.
OrthoDBiEOG73V6KV.
PhylomeDBiQ6MG60.
TreeFamiTF314737.

Family and domain databases

InterProiIPR003198. Amidino_trans.
[Graphical view]
PfamiPF02274. Amidinotransf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MG60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGAS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA
210 220 230 240 250
ALTDHPYASL TLPDDAASDC LFLRPGLPGT TPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKVGA GLSSLCLVLS TRPHC
Length:285
Mass (Da):29,688
Last modified:July 5, 2004 - v1
Checksum:iC28D60FF20CEAEFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83986.1.
BC086443 mRNA. Translation: AAH86443.1.
BC097930 mRNA. Translation: AAH97930.1.
RefSeqiNP_001159408.1. NM_001165936.1.
NP_997697.1. NM_212532.2.
XP_008770936.1. XM_008772714.1.
UniGeneiRn.203056.

Genome annotation databases

EnsembliENSRNOT00000001118; ENSRNOP00000001118; ENSRNOG00000000842.
GeneIDi294239.
KEGGirno:294239.
UCSCiRGD:1302955. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83986.1.
BC086443 mRNA. Translation: AAH86443.1.
BC097930 mRNA. Translation: AAH97930.1.
RefSeqiNP_001159408.1. NM_001165936.1.
NP_997697.1. NM_212532.2.
XP_008770936.1. XM_008772714.1.
UniGeneiRn.203056.

3D structure databases

ProteinModelPortaliQ6MG60.
SMRiQ6MG60. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254630. 1 interaction.
IntActiQ6MG60. 1 interaction.
STRINGi10116.ENSRNOP00000001118.

Proteomic databases

PaxDbiQ6MG60.
PRIDEiQ6MG60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001118; ENSRNOP00000001118; ENSRNOG00000000842.
GeneIDi294239.
KEGGirno:294239.
UCSCiRGD:1302955. rat.

Organism-specific databases

CTDi23564.
RGDi1302955. Ddah2.

Phylogenomic databases

eggNOGiNOG86337.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ6MG60.
KOiK01482.
OMAiMANVCPY.
OrthoDBiEOG73V6KV.
PhylomeDBiQ6MG60.
TreeFamiTF314737.

Enzyme and pathway databases

BRENDAi3.5.3.18. 5301.

Miscellaneous databases

NextBioi637810.
PROiQ6MG60.

Gene expression databases

GenevestigatoriQ6MG60.

Family and domain databases

InterProiIPR003198. Amidino_trans.
[Graphical view]
PfamiPF02274. Amidinotransf. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Thymus.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 106-142; 148-173; 183-194 AND 268-285, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.

Entry informationi

Entry nameiDDAH2_RAT
AccessioniPrimary (citable) accession number: Q6MG60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2004
Last modified: May 27, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.