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Q6MG60

- DDAH2_RAT

UniProt

Q6MG60 - DDAH2_RAT

Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

Ddah2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation By similarity.By similarity

    Catalytic activityi

    N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei171 – 1711Proton donorBy similarity
    Active sitei276 – 2761NucleophileSequence Analysis

    GO - Molecular functioni

    1. amino acid binding Source: RGD
    2. dimethylargininase activity Source: UniProtKB

    GO - Biological processi

    1. arginine metabolic process Source: RGD
    2. citrulline metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
    Short name:
    DDAH-2
    Short name:
    Dimethylarginine dimethylaminohydrolase 2
    Alternative name(s):
    DDAHII
    Dimethylargininase-2
    Gene namesi
    Name:Ddah2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 20

    Organism-specific databases

    RGDi1302955. Ddah2.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity
    Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2PRO_0000270760Add
    BLAST

    Proteomic databases

    PaxDbiQ6MG60.
    PRIDEiQ6MG60.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6MG60.

    Interactioni

    Protein-protein interaction databases

    BioGridi254630. 1 interaction.
    IntActiQ6MG60. 1 interaction.
    STRINGi10116.ENSRNOP00000001118.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6MG60.
    SMRiQ6MG60. Positions 8-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DDAH family.Curated

    Phylogenomic databases

    eggNOGiNOG86337.
    GeneTreeiENSGT00390000009331.
    HOGENOMiHOG000161035.
    HOVERGENiHBG055937.
    InParanoidiQ6MG60.
    KOiK01482.
    OMAiALPFLLH.
    OrthoDBiEOG73V6KV.
    PhylomeDBiQ6MG60.
    TreeFamiTF314737.

    Family and domain databases

    InterProiIPR003198. Amidino_trans.
    [Graphical view]
    PfamiPF02274. Amidinotransf. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6MG60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG    50
    KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV 100
    DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE 150
    IVADTFRDFA VSTVPVSGAS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA 200
    ALTDHPYASL TLPDDAASDC LFLRPGLPGT TPFLLHRGGG DLPNSQEALQ 250
    KLSDVTLVPV SCSELEKVGA GLSSLCLVLS TRPHC 285
    Length:285
    Mass (Da):29,688
    Last modified:July 5, 2004 - v1
    Checksum:iC28D60FF20CEAEFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX883045 Genomic DNA. Translation: CAE83986.1.
    BC086443 mRNA. Translation: AAH86443.1.
    BC097930 mRNA. Translation: AAH97930.1.
    RefSeqiNP_001159408.1. NM_001165936.1.
    NP_997697.1. NM_212532.2.
    UniGeneiRn.203056.

    Genome annotation databases

    EnsembliENSRNOT00000001118; ENSRNOP00000001118; ENSRNOG00000000842.
    GeneIDi294239.
    KEGGirno:294239.
    UCSCiRGD:1302955. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX883045 Genomic DNA. Translation: CAE83986.1 .
    BC086443 mRNA. Translation: AAH86443.1 .
    BC097930 mRNA. Translation: AAH97930.1 .
    RefSeqi NP_001159408.1. NM_001165936.1.
    NP_997697.1. NM_212532.2.
    UniGenei Rn.203056.

    3D structure databases

    ProteinModelPortali Q6MG60.
    SMRi Q6MG60. Positions 8-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 254630. 1 interaction.
    IntActi Q6MG60. 1 interaction.
    STRINGi 10116.ENSRNOP00000001118.

    Chemistry

    BindingDBi Q6MG60.

    Proteomic databases

    PaxDbi Q6MG60.
    PRIDEi Q6MG60.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001118 ; ENSRNOP00000001118 ; ENSRNOG00000000842 .
    GeneIDi 294239.
    KEGGi rno:294239.
    UCSCi RGD:1302955. rat.

    Organism-specific databases

    CTDi 23564.
    RGDi 1302955. Ddah2.

    Phylogenomic databases

    eggNOGi NOG86337.
    GeneTreei ENSGT00390000009331.
    HOGENOMi HOG000161035.
    HOVERGENi HBG055937.
    InParanoidi Q6MG60.
    KOi K01482.
    OMAi ALPFLLH.
    OrthoDBi EOG73V6KV.
    PhylomeDBi Q6MG60.
    TreeFami TF314737.

    Miscellaneous databases

    NextBioi 637810.
    PROi Q6MG60.

    Gene expression databases

    Genevestigatori Q6MG60.

    Family and domain databases

    InterProi IPR003198. Amidino_trans.
    [Graphical view ]
    Pfami PF02274. Amidinotransf. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
      Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
      Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary and Thymus.
    3. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 106-142; 148-173; 183-194 AND 268-285, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiDDAH2_RAT
    AccessioniPrimary (citable) accession number: Q6MG60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3