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Q6MG60 (DDAH2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Short name=DDAH-2
Short name=Dimethylarginine dimethylaminohydrolase 2
EC=3.5.3.18
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene names
Name:Ddah2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation By similarity.

Catalytic activity

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Subcellular location

Cytoplasm By similarity. Mitochondrion By similarity. Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes By similarity.

Sequence similarities

Belongs to the DDAH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
PRO_0000270760

Sites

Active site1711Proton donor By similarity
Active site2761Nucleophile Potential

Sequences

Sequence LengthMass (Da)Tools
Q6MG60 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C28D60FF20CEAEFF

FASTA28529,688
        10         20         30         40         50         60 
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG KLRQRLGLQL 

        70         80         90        100        110        120 
LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV DGVRKALQDL GLRIVEMGDE 

       130        140        150        160        170        180 
NATLDGTDVL FTGREFFVGL SKWTNHRGAE IVADTFRDFA VSTVPVSGAS HLRGLCGMGG 

       190        200        210        220        230        240 
PRTVVAGSSE AAQKAVRAMA ALTDHPYASL TLPDDAASDC LFLRPGLPGT TPFLLHRGGG 

       250        260        270        280 
DLPNSQEALQ KLSDVTLVPV SCSELEKVGA GLSSLCLVLS TRPHC 

« Hide

References

« Hide 'large scale' references
[1]"The genomic sequence and comparative analysis of the rat major histocompatibility complex."
Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary and Thymus.
[3]Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 106-142; 148-173; 183-194 AND 268-285, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain, Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX883045 Genomic DNA. Translation: CAE83986.1.
BC086443 mRNA. Translation: AAH86443.1.
BC097930 mRNA. Translation: AAH97930.1.
RefSeqNP_001159408.1. NM_001165936.1.
NP_997697.1. NM_212532.2.
UniGeneRn.203056.

3D structure databases

ProteinModelPortalQ6MG60.
SMRQ6MG60. Positions 8-280.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid254630. 1 interaction.
IntActQ6MG60. 1 interaction.
STRING10116.ENSRNOP00000001118.

Chemistry

BindingDBQ6MG60.

Proteomic databases

PaxDbQ6MG60.
PRIDEQ6MG60.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000001118; ENSRNOP00000001118; ENSRNOG00000000842.
GeneID294239.
KEGGrno:294239.
UCSCRGD:1302955. rat.

Organism-specific databases

CTD23564.
RGD1302955. Ddah2.

Phylogenomic databases

eggNOGNOG86337.
GeneTreeENSGT00390000009331.
HOGENOMHOG000161035.
HOVERGENHBG055937.
InParanoidQ6MG60.
KOK01482.
OMAALPFLLH.
OrthoDBEOG73V6KV.
PhylomeDBQ6MG60.
TreeFamTF314737.

Gene expression databases

GenevestigatorQ6MG60.

Family and domain databases

InterProIPR003198. Amidino_trans.
[Graphical view]
PfamPF02274. Amidinotransf. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio637810.
PROQ6MG60.

Entry information

Entry nameDDAH2_RAT
AccessionPrimary (citable) accession number: Q6MG60
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families