Q6MG49 (BAG6_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 71.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Large proline-rich protein BAG6 Alternative name(s): BCL2-associated athanogene 6 HLA-B-associated transcript 3 | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1146 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane By similarity. Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) By similarity. Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity By similarity. |
| Subunit structure | Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain By similarity. |
| Subcellular location | Cytoplasm › cytosol By similarity. Nucleus By similarity. Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells By similarity. |
| Post-translational modification | Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis By similarity. |
| Sequence similarities | Contains 1 ubiquitin-like domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q6MG49-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q6MG49-2) The sequence of this isoform differs from the canonical sequence as follows: 522-522: Missing. 1067-1115: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1146 | 1146 | Large proline-rich protein BAG6 | PRO_0000114899 | |||||
Regions | |||||||||
| Domain | 17 – 92 | 76 | Ubiquitin-like | ||||||
| Repeat | 236 – 265 | 30 | 1 | ||||||
| Repeat | 410 – 438 | 29 | 2 | ||||||
| Repeat | 589 – 616 | 28 | 3 | ||||||
| Repeat | 622 – 650 | 29 | 4 | ||||||
| Region | 237 – 650 | 414 | 4 X 29 AA approximate repeats | ||||||
| Compositional bias | 196 – 268 | 73 | Pro-rich | ||||||
| Compositional bias | 389 – 711 | 323 | Pro-rich | ||||||
| Compositional bias | 558 – 602 | 45 | Ala-rich | ||||||
Sites | |||||||||
| Site | 1015 – 1016 | 2 | Cleavage; by caspase-3 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 978 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 987 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1095 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1131 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 522 | 1 | Missing in isoform 2. | VSP_040420 | |||||
| Alternative sequence | 1067 – 1115 | 49 | Missing in isoform 2. | VSP_040421 | |||||
Experimental info | |||||||||
| Sequence conflict | 4 | 1 | S → N in BAA76607. Ref.1 | ||||||
| Sequence conflict | 11 | 1 | M → V in BAA76607. Ref.1 | ||||||
| Sequence conflict | 47 | 1 | A → R in BAA76607. Ref.1 | ||||||
| Sequence conflict | 74 | 1 | D → E in BAA76607. Ref.1 | ||||||
| Sequence conflict | 93 | 1 | Q → H in BAA76607. Ref.1 | ||||||
| Sequence conflict | 114 | 1 | L → C in BAA76607. Ref.1 | ||||||
| Sequence conflict | 189 | 1 | T → N in BAA76607. Ref.1 | ||||||
| Sequence conflict | 264 – 265 | 2 | NH → TQ in BAA76607. Ref.1 | ||||||
| Sequence conflict | 567 | 1 | T → TAQ in BAA76607. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of rat BAT3 cDNA." Ozaki T., Hanaoka E., Naka M., Nakagawara A., Sakiyama S. DNA Cell Biol. 18:503-512(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [2] | "The genomic sequence and comparative analysis of the rat major histocompatibility complex." Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H. Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway. |
| [3] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB018791 mRNA. Translation: BAA76607.1. BX883045 Genomic DNA. Translation: CAE83997.1. CH474121 Genomic DNA. Translation: EDL83534.1. BC100141 mRNA. Translation: AAI00142.1. |
| IPI | IPI00203181. IPI00215312. |
| RefSeq | NP_001029140.1. NM_001033968.1. NP_446061.2. NM_053609.2. |
| UniGene | Rn.203343. |
3D structure databases | |
| ProteinModelPortal | Q6MG49. |
| SMR | Q6MG49. Positions 17-101. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q6MG49. 1 interaction. |
| MINT | MINT-4566953. |
| STRING | 10116.ENSRNOP00000057557. |
PTM databases | |
| PhosphoSite | Q6MG49. |
Proteomic databases | |
| PaxDb | Q6MG49. |
| PRIDE | Q6MG49. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000001129; ENSRNOP00000001129; ENSRNOG00000000851. ENSRNOT00000060832; ENSRNOP00000057557; ENSRNOG00000000851. |
| GeneID | 94342. |
| KEGG | rno:94342. |
| UCSC | RGD:71064. rat. |
Organism-specific databases | |
| CTD | 7917. |
| RGD | 71064. Bag6. |
Phylogenomic databases | |
| eggNOG | NOG297129. |
| GeneTree | ENSGT00390000016199. |
| HOGENOM | HOG000095177. |
| HOVERGEN | HBG002193. |
| InParanoid | Q498N5. |
| OMA | PQRENAS. |
Gene expression databases | |
| ArrayExpress | Q6MG49. |
| Genevestigator | Q6MG49. |
| GermOnline | ENSRNOG00000000851. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR021925. DUF3538. IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019955. Ubiquitin_supergroup. [Graphical view] |
| Pfam | PF12057. DUF3538. 1 hit. PF00240. ubiquitin. 1 hit. [Graphical view] |
| SMART | SM00213. UBQ. 1 hit. [Graphical view] |
| PROSITE | PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 617937. |
Entry information
| Entry name | BAG6_RAT | ||||||||
| Accession | Primary (citable) accession number: Q6MG49 Secondary accession number(s): Q498N5, Q9WTN8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |