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Q6MG49 (BAG6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large proline-rich protein BAG6
Alternative name(s):
BCL2-associated athanogene 6
HLA-B-associated transcript 3
Gene names
Name:Bag6
Synonyms:Bat3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1146 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane By similarity.

Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) By similarity.

Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity By similarity.

Subunit structure

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Nucleus By similarity. Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells By similarity.

Post-translational modification

Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis By similarity.

Sequence similarities

Contains 1 ubiquitin-like domain.

Ontologies

Keywords
   Biological processApoptosis
Differentiation
Immunity
Spermatogenesis
Transport
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   Molecular functionChaperone
Chromatin regulator
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from sequence or structural similarity. Source: UniProtKB

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin modification

Inferred from electronic annotation. Source: UniProtKB-KW

embryo development

Inferred from sequence or structural similarity. Source: UniProtKB

immune system process

Inferred from electronic annotation. Source: UniProtKB-KW

internal peptidyl-lysine acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

Inferred from sequence or structural similarity. Source: UniProtKB

intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

Inferred from sequence or structural similarity. Source: UniProtKB

kidney development

Inferred from sequence or structural similarity. Source: UniProtKB

lung development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of proteasomal ubiquitin-dependent protein catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of proteolysis

Inferred from sequence or structural similarity. Source: UniProtKB

protein stabilization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell proliferation

Inferred from expression pattern Ref.1. Source: RGD

spermatogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

synaptonemal complex assembly

Inferred from sequence or structural similarity. Source: UniProtKB

tail-anchored membrane protein insertion into ER membrane

Inferred from sequence or structural similarity. Source: UniProtKB

transport

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-dependent protein catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentBAT3 complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.1. Source: RGD

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionpolyubiquitin binding

Inferred from sequence or structural similarity. Source: UniProtKB

proteasome binding

Inferred from sequence or structural similarity. Source: UniProtKB

ribosome binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6MG49-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6MG49-2)

The sequence of this isoform differs from the canonical sequence as follows:
     522-522: Missing.
     1067-1115: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11461146Large proline-rich protein BAG6
PRO_0000114899

Regions

Domain17 – 9276Ubiquitin-like
Repeat236 – 265301
Repeat410 – 438292
Repeat589 – 616283
Repeat622 – 650294
Region237 – 6504144 X 29 AA approximate repeats
Compositional bias196 – 26873Pro-rich
Compositional bias389 – 711323Pro-rich
Compositional bias558 – 60245Ala-rich

Sites

Site1015 – 10162Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue9781Phosphoserine By similarity
Modified residue9871Phosphoserine By similarity
Modified residue10951Phosphoserine By similarity
Modified residue11311Phosphoserine By similarity

Natural variations

Alternative sequence5221Missing in isoform 2.
VSP_040420
Alternative sequence1067 – 111549Missing in isoform 2.
VSP_040421

Experimental info

Sequence conflict41S → N in BAA76607. Ref.1
Sequence conflict111M → V in BAA76607. Ref.1
Sequence conflict471A → R in BAA76607. Ref.1
Sequence conflict741D → E in BAA76607. Ref.1
Sequence conflict931Q → H in BAA76607. Ref.1
Sequence conflict1141L → C in BAA76607. Ref.1
Sequence conflict1891T → N in BAA76607. Ref.1
Sequence conflict264 – 2652NH → TQ in BAA76607. Ref.1
Sequence conflict5671T → TAQ in BAA76607. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: 9CAA4CD9C04F3C29

FASTA1,146120,012
        10         20         30         40         50         60 
MEPSDSTSTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 

        70         80         90        100        110        120 
YQGRVLQDDK KLQDYNVGGK VIHLVERAPP QTQLPSGASS GTGSASATHG GGPLPGTRGP 

       130        140        150        160        170        180 
GASGHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 

       190        200        210        220        230        240 
SRMECRGGTQ AQASQPPPQT PTVASETVAL NSQTSEPVES EAPPREPMES EEMEERPPTQ 

       250        260        270        280        290        300 
TPELPPSGPA PAGPAPAPET NAPNHPSPAE HVEVLQELQR LQRRLQPFLQ RYCEVLGAAA 

       310        320        330        340        350        360 
TTDYNNNHEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL HVVRPMSHYT 

       370        380        390        400        410        420 
TPMVLQQAAI PIQINVGTTV TMTGNGARPP PAPGAEAASP GSGQASSLPP SSATVDSSTE 

       430        440        450        460        470        480 
GAPPPGPAPP PATSHPRVIR ISHQSVEPVV MMHMNIQDSG AQPGGVPSAP TGPLGPPGHG 

       490        500        510        520        530        540 
QSLGQQVPGF PTAPTRVVIA RPTPPQARPS HPGGPPVSGA LQGAGLGTNT SLAQMVSGLV 

       550        560        570        580        590        600 
GQLLMQPVLV AQGTPGMAPA SASAPATAQA QAPAPAPAPA PAPATASASA GTTNTATTAG 

       610        620        630        640        650        660 
PAPGGPAQPP PPQPSAADLQ FSQLLGNLLG PAGPGAGGPS LASPTITVAV PGVPAFLQGM 

       670        680        690        700        710        720 
TEFLQASQAA PPPPPPPPPP PPAPEQQTTP PPGSPSGGTA SPGGLGPESL PPEFFTSVVQ 

       730        740        750        760        770        780 
GVLSSLLGSL GARAGSSESI AAFIQRLSGS SNIFEPGADG ALGFFGALLS LLCQNFSMVD 

       790        800        810        820        830        840 
VVMLLHGHFQ PLQRLQPQLR SFFHQHYLGG QEPTSSNIRM ATHTLITGLE EYVRESFSLV 

       850        860        870        880        890        900 
QVQPGVDIIR TNLEFLQEQF NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG 

       910        920        930        940        950        960 
QQMELAAVIN GRIRRMSRGV NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI LRYVRRIGDP 

       970        980        990       1000       1010       1020 
PQALPEEPME VQGAERTSPE PQREDASPAP GTTAEEAMSR GPPPAPEGGS RDEQDGASAD 

      1030       1040       1050       1060       1070       1080 
AEPWAAAVPP EWVPIIQQDI QSQRKVKPQP PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA 

      1090       1100       1110       1120       1130       1140 
VSRAAKAAGA RPLTSPESLS RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAHR 


AFADDP 

« Hide

Isoform 2 [UniParc].

Checksum: E372D70E024D8F15
Show »

FASTA1,096114,647

References

« Hide 'large scale' references
[1]"Cloning and characterization of rat BAT3 cDNA."
Ozaki T., Hanaoka E., Naka M., Nakagawara A., Sakiyama S.
DNA Cell Biol. 18:503-512(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The genomic sequence and comparative analysis of the rat major histocompatibility complex."
Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB018791 mRNA. Translation: BAA76607.1.
BX883045 Genomic DNA. Translation: CAE83997.1.
CH474121 Genomic DNA. Translation: EDL83534.1.
BC100141 mRNA. Translation: AAI00142.1.
RefSeqNP_001029140.1. NM_001033968.1. [Q6MG49-1]
NP_446061.2. NM_053609.2. [Q6MG49-2]
UniGeneRn.203343.

3D structure databases

ProteinModelPortalQ6MG49.
SMRQ6MG49. Positions 17-101.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250197. 1 interaction.
IntActQ6MG49. 2 interactions.
MINTMINT-4566953.
STRING10116.ENSRNOP00000057557.

PTM databases

PhosphoSiteQ6MG49.

Proteomic databases

PaxDbQ6MG49.
PRIDEQ6MG49.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000001129; ENSRNOP00000001129; ENSRNOG00000000851. [Q6MG49-2]
ENSRNOT00000060832; ENSRNOP00000057557; ENSRNOG00000000851. [Q6MG49-1]
GeneID94342.
KEGGrno:94342.
UCSCRGD:71064. rat. [Q6MG49-1]

Organism-specific databases

CTD7917.
RGD71064. Bag6.

Phylogenomic databases

eggNOGNOG297129.
GeneTreeENSGT00390000016199.
HOGENOMHOG000095177.
HOVERGENHBG002193.
InParanoidQ498N5.
OMAQEPTPGN.
OrthoDBEOG7B5WXH.
PhylomeDBQ6MG49.
TreeFamTF328437.

Gene expression databases

GenevestigatorQ6MG49.

Family and domain databases

InterProIPR021925. DUF3538.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamPF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
PROSITEPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617937.
PROQ6MG49.

Entry information

Entry nameBAG6_RAT
AccessionPrimary (citable) accession number: Q6MG49
Secondary accession number(s): Q498N5, Q9WTN8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 11, 2011
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families