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Q6MG49

- BAG6_RAT

UniProt

Q6MG49 - BAG6_RAT

Protein

Large proline-rich protein BAG6

Gene

Bag6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane By similarity.By similarity
    Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) By similarity.By similarity
    Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1015 – 10162Cleavage; by caspase-3By similarity

    GO - Molecular functioni

    1. polyubiquitin binding Source: UniProtKB
    2. proteasome binding Source: UniProtKB
    3. ribosome binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: UniProtKB
    2. cell differentiation Source: UniProtKB-KW
    3. chromatin modification Source: UniProtKB-KW
    4. embryo development Source: UniProtKB
    5. immune system process Source: UniProtKB-KW
    6. internal peptidyl-lysine acetylation Source: UniProtKB
    7. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
    8. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
    9. kidney development Source: UniProtKB
    10. lung development Source: UniProtKB
    11. negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    12. negative regulation of proteolysis Source: UniProtKB
    13. protein stabilization Source: UniProtKB
    14. regulation of cell proliferation Source: RGD
    15. spermatogenesis Source: UniProtKB
    16. synaptonemal complex assembly Source: UniProtKB
    17. tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
    18. transport Source: UniProtKB-KW
    19. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone, Chromatin regulator

    Keywords - Biological processi

    Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large proline-rich protein BAG6
    Alternative name(s):
    BCL2-associated athanogene 6
    HLA-B-associated transcript 3
    Gene namesi
    Name:Bag6
    Synonyms:Bat3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 20

    Organism-specific databases

    RGDi71064. Bag6.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Nucleus By similarity
    Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells By similarity.By similarity

    GO - Cellular componenti

    1. BAT3 complex Source: UniProtKB
    2. cytoplasm Source: RGD
    3. cytosol Source: UniProtKB
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11461146Large proline-rich protein BAG6PRO_0000114899Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei978 – 9781PhosphoserineBy similarity
    Modified residuei987 – 9871PhosphoserineBy similarity
    Modified residuei1095 – 10951PhosphoserineBy similarity
    Modified residuei1131 – 11311PhosphoserineBy similarity

    Post-translational modificationi

    Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ6MG49.
    PRIDEiQ6MG49.

    PTM databases

    PhosphoSiteiQ6MG49.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6MG49.

    Interactioni

    Subunit structurei

    Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250197. 1 interaction.
    IntActiQ6MG49. 2 interactions.
    MINTiMINT-4566953.
    STRINGi10116.ENSRNOP00000057557.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6MG49.
    SMRiQ6MG49. Positions 17-101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 9276Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Repeati236 – 265301Add
    BLAST
    Repeati410 – 438292Add
    BLAST
    Repeati589 – 616283Add
    BLAST
    Repeati622 – 650294Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 6504144 X 29 AA approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi196 – 26873Pro-richAdd
    BLAST
    Compositional biasi389 – 711323Pro-richAdd
    BLAST
    Compositional biasi558 – 60245Ala-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG297129.
    GeneTreeiENSGT00390000016199.
    HOGENOMiHOG000095177.
    HOVERGENiHBG002193.
    InParanoidiQ498N5.
    OMAiQEPTPGN.
    OrthoDBiEOG7B5WXH.
    PhylomeDBiQ6MG49.
    TreeFamiTF328437.

    Family and domain databases

    InterProiIPR021925. DUF3538.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PfamiPF12057. DUF3538. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6MG49-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPSDSTSTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV     50
    SIPSEKQRLI YQGRVLQDDK KLQDYNVGGK VIHLVERAPP QTQLPSGASS 100
    GTGSASATHG GGPLPGTRGP GASGHDRNAN SYVMVGTFNL PSDGSAVDVH 150
    INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL SRMECRGGTQ AQASQPPPQT 200
    PTVASETVAL NSQTSEPVES EAPPREPMES EEMEERPPTQ TPELPPSGPA 250
    PAGPAPAPET NAPNHPSPAE HVEVLQELQR LQRRLQPFLQ RYCEVLGAAA 300
    TTDYNNNHEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL 350
    HVVRPMSHYT TPMVLQQAAI PIQINVGTTV TMTGNGARPP PAPGAEAASP 400
    GSGQASSLPP SSATVDSSTE GAPPPGPAPP PATSHPRVIR ISHQSVEPVV 450
    MMHMNIQDSG AQPGGVPSAP TGPLGPPGHG QSLGQQVPGF PTAPTRVVIA 500
    RPTPPQARPS HPGGPPVSGA LQGAGLGTNT SLAQMVSGLV GQLLMQPVLV 550
    AQGTPGMAPA SASAPATAQA QAPAPAPAPA PAPATASASA GTTNTATTAG 600
    PAPGGPAQPP PPQPSAADLQ FSQLLGNLLG PAGPGAGGPS LASPTITVAV 650
    PGVPAFLQGM TEFLQASQAA PPPPPPPPPP PPAPEQQTTP PPGSPSGGTA 700
    SPGGLGPESL PPEFFTSVVQ GVLSSLLGSL GARAGSSESI AAFIQRLSGS 750
    SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR 800
    SFFHQHYLGG QEPTSSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR 850
    TNLEFLQEQF NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG 900
    QQMELAAVIN GRIRRMSRGV NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI 950
    LRYVRRIGDP PQALPEEPME VQGAERTSPE PQREDASPAP GTTAEEAMSR 1000
    GPPPAPEGGS RDEQDGASAD AEPWAAAVPP EWVPIIQQDI QSQRKVKPQP 1050
    PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS 1100
    RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAHR AFADDP 1146
    Length:1,146
    Mass (Da):120,012
    Last modified:January 11, 2011 - v2
    Checksum:i9CAA4CD9C04F3C29
    GO
    Isoform 2 (identifier: Q6MG49-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         522-522: Missing.
         1067-1115: Missing.

    Show »
    Length:1,096
    Mass (Da):114,647
    Checksum:iE372D70E024D8F15
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41S → N in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti11 – 111M → V in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti47 – 471A → R in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti74 – 741D → E in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti93 – 931Q → H in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti114 – 1141L → C in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti189 – 1891T → N in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti264 – 2652NH → TQ in BAA76607. (PubMed:10390159)Curated
    Sequence conflicti567 – 5671T → TAQ in BAA76607. (PubMed:10390159)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei522 – 5221Missing in isoform 2. 1 PublicationVSP_040420
    Alternative sequencei1067 – 111549Missing in isoform 2. 1 PublicationVSP_040421Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018791 mRNA. Translation: BAA76607.1.
    BX883045 Genomic DNA. Translation: CAE83997.1.
    CH474121 Genomic DNA. Translation: EDL83534.1.
    BC100141 mRNA. Translation: AAI00142.1.
    RefSeqiNP_001029140.1. NM_001033968.1. [Q6MG49-1]
    NP_446061.2. NM_053609.2. [Q6MG49-2]
    UniGeneiRn.203343.

    Genome annotation databases

    EnsembliENSRNOT00000001129; ENSRNOP00000001129; ENSRNOG00000000851. [Q6MG49-2]
    ENSRNOT00000060832; ENSRNOP00000057557; ENSRNOG00000000851. [Q6MG49-1]
    GeneIDi94342.
    KEGGirno:94342.
    UCSCiRGD:71064. rat. [Q6MG49-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018791 mRNA. Translation: BAA76607.1 .
    BX883045 Genomic DNA. Translation: CAE83997.1 .
    CH474121 Genomic DNA. Translation: EDL83534.1 .
    BC100141 mRNA. Translation: AAI00142.1 .
    RefSeqi NP_001029140.1. NM_001033968.1. [Q6MG49-1 ]
    NP_446061.2. NM_053609.2. [Q6MG49-2 ]
    UniGenei Rn.203343.

    3D structure databases

    ProteinModelPortali Q6MG49.
    SMRi Q6MG49. Positions 17-101.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250197. 1 interaction.
    IntActi Q6MG49. 2 interactions.
    MINTi MINT-4566953.
    STRINGi 10116.ENSRNOP00000057557.

    PTM databases

    PhosphoSitei Q6MG49.

    Proteomic databases

    PaxDbi Q6MG49.
    PRIDEi Q6MG49.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001129 ; ENSRNOP00000001129 ; ENSRNOG00000000851 . [Q6MG49-2 ]
    ENSRNOT00000060832 ; ENSRNOP00000057557 ; ENSRNOG00000000851 . [Q6MG49-1 ]
    GeneIDi 94342.
    KEGGi rno:94342.
    UCSCi RGD:71064. rat. [Q6MG49-1 ]

    Organism-specific databases

    CTDi 7917.
    RGDi 71064. Bag6.

    Phylogenomic databases

    eggNOGi NOG297129.
    GeneTreei ENSGT00390000016199.
    HOGENOMi HOG000095177.
    HOVERGENi HBG002193.
    InParanoidi Q498N5.
    OMAi QEPTPGN.
    OrthoDBi EOG7B5WXH.
    PhylomeDBi Q6MG49.
    TreeFami TF328437.

    Miscellaneous databases

    NextBioi 617937.
    PROi Q6MG49.

    Gene expression databases

    Genevestigatori Q6MG49.

    Family and domain databases

    InterProi IPR021925. DUF3538.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    Pfami PF12057. DUF3538. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
      Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
      Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.

    Entry informationi

    Entry nameiBAG6_RAT
    AccessioniPrimary (citable) accession number: Q6MG49
    Secondary accession number(s): Q498N5, Q9WTN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3