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Q6MG49

- BAG6_RAT

UniProt

Q6MG49 - BAG6_RAT

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Protein

Large proline-rich protein BAG6

Gene

Bag6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Moreover, it regulates the stability and the degradation of proteins by the proteasome. For instance, it is required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. It is also involved in ubiquitin-mediated proteasomal degradation of proteins of the secretory pathway that are mislocalized to the cytosol. Binds the mislocalized proteins, preventing their aggregation in the cytosol, and promotes their ubiquitination. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2.By similarity
Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) (By similarity).By similarity
Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cells (NK) activation and cytotoxicity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1015 – 10162Cleavage; by caspase-3By similarity

GO - Molecular functioni

  1. polyubiquitin binding Source: UniProtKB
  2. proteasome binding Source: UniProtKB
  3. ribosome binding Source: UniProtKB

GO - Biological processi

  1. brain development Source: UniProtKB
  2. cell differentiation Source: UniProtKB-KW
  3. chromatin modification Source: UniProtKB-KW
  4. embryo development Source: UniProtKB
  5. immune system process Source: UniProtKB-KW
  6. internal peptidyl-lysine acetylation Source: UniProtKB
  7. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  8. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  9. kidney development Source: UniProtKB
  10. lung development Source: UniProtKB
  11. negative regulation of apoptotic process Source: Ensembl
  12. negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  13. negative regulation of proteolysis Source: UniProtKB
  14. protein stabilization Source: UniProtKB
  15. regulation of cell proliferation Source: RGD
  16. spermatogenesis Source: UniProtKB
  17. synaptonemal complex assembly Source: UniProtKB
  18. tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
  19. transport Source: UniProtKB-KW
  20. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Chromatin regulator

Keywords - Biological processi

Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Large proline-rich protein BAG6
Alternative name(s):
BCL2-associated athanogene 6
HLA-B-associated transcript 3
Gene namesi
Name:Bag6
Synonyms:Bat3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi71064. Bag6.

Subcellular locationi

Cytoplasmcytosol By similarity. Nucleus By similarity
Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells (By similarity).By similarity

GO - Cellular componenti

  1. BAT3 complex Source: UniProtKB
  2. cytoplasm Source: RGD
  3. cytosol Source: UniProtKB
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11461146Large proline-rich protein BAG6PRO_0000114899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei978 – 9781PhosphoserineBy similarity
Modified residuei987 – 9871PhosphoserineBy similarity
Modified residuei1095 – 10951PhosphoserineBy similarity
Modified residuei1131 – 11311PhosphoserineBy similarity

Post-translational modificationi

Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6MG49.
PRIDEiQ6MG49.

PTM databases

PhosphoSiteiQ6MG49.

Expressioni

Gene expression databases

GenevestigatoriQ6MG49.

Interactioni

Subunit structurei

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with NCR3. Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol.By similarity

Protein-protein interaction databases

BioGridi250197. 1 interaction.
IntActiQ6MG49. 2 interactions.
MINTiMINT-4566953.
STRINGi10116.ENSRNOP00000057557.

Structurei

3D structure databases

ProteinModelPortaliQ6MG49.
SMRiQ6MG49. Positions 17-101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 9276Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST
Repeati236 – 265301Add
BLAST
Repeati410 – 438292Add
BLAST
Repeati589 – 616283Add
BLAST
Repeati622 – 650294Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 6504144 X 29 AA approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi196 – 26873Pro-richAdd
BLAST
Compositional biasi389 – 711323Pro-richAdd
BLAST
Compositional biasi558 – 60245Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG297129.
GeneTreeiENSGT00390000016199.
HOGENOMiHOG000095177.
HOVERGENiHBG002193.
InParanoidiQ6MG49.
OMAiQEPTPGN.
OrthoDBiEOG7B5WXH.
PhylomeDBiQ6MG49.
TreeFamiTF328437.

Family and domain databases

InterProiIPR021925. DUF3538.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6MG49) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSDSTSTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV
60 70 80 90 100
SIPSEKQRLI YQGRVLQDDK KLQDYNVGGK VIHLVERAPP QTQLPSGASS
110 120 130 140 150
GTGSASATHG GGPLPGTRGP GASGHDRNAN SYVMVGTFNL PSDGSAVDVH
160 170 180 190 200
INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL SRMECRGGTQ AQASQPPPQT
210 220 230 240 250
PTVASETVAL NSQTSEPVES EAPPREPMES EEMEERPPTQ TPELPPSGPA
260 270 280 290 300
PAGPAPAPET NAPNHPSPAE HVEVLQELQR LQRRLQPFLQ RYCEVLGAAA
310 320 330 340 350
TTDYNNNHEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL
360 370 380 390 400
HVVRPMSHYT TPMVLQQAAI PIQINVGTTV TMTGNGARPP PAPGAEAASP
410 420 430 440 450
GSGQASSLPP SSATVDSSTE GAPPPGPAPP PATSHPRVIR ISHQSVEPVV
460 470 480 490 500
MMHMNIQDSG AQPGGVPSAP TGPLGPPGHG QSLGQQVPGF PTAPTRVVIA
510 520 530 540 550
RPTPPQARPS HPGGPPVSGA LQGAGLGTNT SLAQMVSGLV GQLLMQPVLV
560 570 580 590 600
AQGTPGMAPA SASAPATAQA QAPAPAPAPA PAPATASASA GTTNTATTAG
610 620 630 640 650
PAPGGPAQPP PPQPSAADLQ FSQLLGNLLG PAGPGAGGPS LASPTITVAV
660 670 680 690 700
PGVPAFLQGM TEFLQASQAA PPPPPPPPPP PPAPEQQTTP PPGSPSGGTA
710 720 730 740 750
SPGGLGPESL PPEFFTSVVQ GVLSSLLGSL GARAGSSESI AAFIQRLSGS
760 770 780 790 800
SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR
810 820 830 840 850
SFFHQHYLGG QEPTSSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR
860 870 880 890 900
TNLEFLQEQF NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG
910 920 930 940 950
QQMELAAVIN GRIRRMSRGV NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI
960 970 980 990 1000
LRYVRRIGDP PQALPEEPME VQGAERTSPE PQREDASPAP GTTAEEAMSR
1010 1020 1030 1040 1050
GPPPAPEGGS RDEQDGASAD AEPWAAAVPP EWVPIIQQDI QSQRKVKPQP
1060 1070 1080 1090 1100
PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS
1110 1120 1130 1140
RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAHR AFADDP
Length:1,146
Mass (Da):120,012
Last modified:January 11, 2011 - v2
Checksum:i9CAA4CD9C04F3C29
GO
Isoform 2 (identifier: Q6MG49-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     522-522: Missing.
     1067-1115: Missing.

Show »
Length:1,096
Mass (Da):114,647
Checksum:iE372D70E024D8F15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → N in BAA76607. (PubMed:10390159)Curated
Sequence conflicti11 – 111M → V in BAA76607. (PubMed:10390159)Curated
Sequence conflicti47 – 471A → R in BAA76607. (PubMed:10390159)Curated
Sequence conflicti74 – 741D → E in BAA76607. (PubMed:10390159)Curated
Sequence conflicti93 – 931Q → H in BAA76607. (PubMed:10390159)Curated
Sequence conflicti114 – 1141L → C in BAA76607. (PubMed:10390159)Curated
Sequence conflicti189 – 1891T → N in BAA76607. (PubMed:10390159)Curated
Sequence conflicti264 – 2652NH → TQ in BAA76607. (PubMed:10390159)Curated
Sequence conflicti567 – 5671T → TAQ in BAA76607. (PubMed:10390159)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei522 – 5221Missing in isoform 2. 1 PublicationVSP_040420
Alternative sequencei1067 – 111549Missing in isoform 2. 1 PublicationVSP_040421Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018791 mRNA. Translation: BAA76607.1.
BX883045 Genomic DNA. Translation: CAE83997.1.
CH474121 Genomic DNA. Translation: EDL83534.1.
BC100141 mRNA. Translation: AAI00142.1.
RefSeqiNP_001029140.1. NM_001033968.1. [Q6MG49-1]
NP_446061.2. NM_053609.2. [Q6MG49-2]
UniGeneiRn.203343.

Genome annotation databases

EnsembliENSRNOT00000001129; ENSRNOP00000001129; ENSRNOG00000000851. [Q6MG49-2]
ENSRNOT00000060832; ENSRNOP00000057557; ENSRNOG00000000851. [Q6MG49-1]
GeneIDi94342.
KEGGirno:94342.
UCSCiRGD:71064. rat. [Q6MG49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018791 mRNA. Translation: BAA76607.1 .
BX883045 Genomic DNA. Translation: CAE83997.1 .
CH474121 Genomic DNA. Translation: EDL83534.1 .
BC100141 mRNA. Translation: AAI00142.1 .
RefSeqi NP_001029140.1. NM_001033968.1. [Q6MG49-1 ]
NP_446061.2. NM_053609.2. [Q6MG49-2 ]
UniGenei Rn.203343.

3D structure databases

ProteinModelPortali Q6MG49.
SMRi Q6MG49. Positions 17-101.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250197. 1 interaction.
IntActi Q6MG49. 2 interactions.
MINTi MINT-4566953.
STRINGi 10116.ENSRNOP00000057557.

PTM databases

PhosphoSitei Q6MG49.

Proteomic databases

PaxDbi Q6MG49.
PRIDEi Q6MG49.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001129 ; ENSRNOP00000001129 ; ENSRNOG00000000851 . [Q6MG49-2 ]
ENSRNOT00000060832 ; ENSRNOP00000057557 ; ENSRNOG00000000851 . [Q6MG49-1 ]
GeneIDi 94342.
KEGGi rno:94342.
UCSCi RGD:71064. rat. [Q6MG49-1 ]

Organism-specific databases

CTDi 7917.
RGDi 71064. Bag6.

Phylogenomic databases

eggNOGi NOG297129.
GeneTreei ENSGT00390000016199.
HOGENOMi HOG000095177.
HOVERGENi HBG002193.
InParanoidi Q6MG49.
OMAi QEPTPGN.
OrthoDBi EOG7B5WXH.
PhylomeDBi Q6MG49.
TreeFami TF328437.

Miscellaneous databases

NextBioi 617937.
PROi Q6MG49.

Gene expression databases

Genevestigatori Q6MG49.

Family and domain databases

InterProi IPR021925. DUF3538.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view ]
Pfami PF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
SMARTi SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiBAG6_RAT
AccessioniPrimary (citable) accession number: Q6MG49
Secondary accession number(s): Q498N5, Q9WTN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 11, 2011
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3