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Q6MG49

- BAG6_RAT

UniProt

Q6MG49 - BAG6_RAT

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Protein
Large proline-rich protein BAG6
Gene
Bag6, Bat3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane By similarity.
Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) By similarity.
Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1015 – 10162Cleavage; by caspase-3 By similarity

GO - Molecular functioni

  1. polyubiquitin binding Source: UniProtKB
  2. proteasome binding Source: UniProtKB
  3. ribosome binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. brain development Source: UniProtKB
  2. cell differentiation Source: UniProtKB-KW
  3. chromatin modification Source: UniProtKB-KW
  4. embryo development Source: UniProtKB
  5. immune system process Source: UniProtKB-KW
  6. internal peptidyl-lysine acetylation Source: UniProtKB
  7. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  8. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  9. kidney development Source: UniProtKB
  10. lung development Source: UniProtKB
  11. negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  12. negative regulation of proteolysis Source: UniProtKB
  13. protein stabilization Source: UniProtKB
  14. regulation of cell proliferation Source: RGD
  15. spermatogenesis Source: UniProtKB
  16. synaptonemal complex assembly Source: UniProtKB
  17. tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
  18. transport Source: UniProtKB-KW
  19. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Chromatin regulator

Keywords - Biological processi

Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Large proline-rich protein BAG6
Alternative name(s):
BCL2-associated athanogene 6
HLA-B-associated transcript 3
Gene namesi
Name:Bag6
Synonyms:Bat3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi71064. Bag6.

Subcellular locationi

Cytoplasmcytosol By similarity. Nucleus By similarity
Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells By similarity.

GO - Cellular componenti

  1. BAT3 complex Source: UniProtKB
  2. cytoplasm Source: RGD
  3. cytosol Source: UniProtKB
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11461146Large proline-rich protein BAG6
PRO_0000114899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei978 – 9781Phosphoserine By similarity
Modified residuei987 – 9871Phosphoserine By similarity
Modified residuei1095 – 10951Phosphoserine By similarity
Modified residuei1131 – 11311Phosphoserine By similarity

Post-translational modificationi

Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6MG49.
PRIDEiQ6MG49.

PTM databases

PhosphoSiteiQ6MG49.

Expressioni

Gene expression databases

GenevestigatoriQ6MG49.

Interactioni

Subunit structurei

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain By similarity.

Protein-protein interaction databases

BioGridi250197. 1 interaction.
IntActiQ6MG49. 2 interactions.
MINTiMINT-4566953.
STRINGi10116.ENSRNOP00000057557.

Structurei

3D structure databases

ProteinModelPortaliQ6MG49.
SMRiQ6MG49. Positions 17-101.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 9276Ubiquitin-like
Add
BLAST
Repeati236 – 265301
Add
BLAST
Repeati410 – 438292
Add
BLAST
Repeati589 – 616283
Add
BLAST
Repeati622 – 650294
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 6504144 X 29 AA approximate repeats
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi196 – 26873Pro-rich
Add
BLAST
Compositional biasi389 – 711323Pro-rich
Add
BLAST
Compositional biasi558 – 60245Ala-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG297129.
GeneTreeiENSGT00390000016199.
HOGENOMiHOG000095177.
HOVERGENiHBG002193.
InParanoidiQ498N5.
OMAiQEPTPGN.
OrthoDBiEOG7B5WXH.
PhylomeDBiQ6MG49.
TreeFamiTF328437.

Family and domain databases

InterProiIPR021925. DUF3538.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6MG49-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEPSDSTSTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV     50
SIPSEKQRLI YQGRVLQDDK KLQDYNVGGK VIHLVERAPP QTQLPSGASS 100
GTGSASATHG GGPLPGTRGP GASGHDRNAN SYVMVGTFNL PSDGSAVDVH 150
INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL SRMECRGGTQ AQASQPPPQT 200
PTVASETVAL NSQTSEPVES EAPPREPMES EEMEERPPTQ TPELPPSGPA 250
PAGPAPAPET NAPNHPSPAE HVEVLQELQR LQRRLQPFLQ RYCEVLGAAA 300
TTDYNNNHEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL 350
HVVRPMSHYT TPMVLQQAAI PIQINVGTTV TMTGNGARPP PAPGAEAASP 400
GSGQASSLPP SSATVDSSTE GAPPPGPAPP PATSHPRVIR ISHQSVEPVV 450
MMHMNIQDSG AQPGGVPSAP TGPLGPPGHG QSLGQQVPGF PTAPTRVVIA 500
RPTPPQARPS HPGGPPVSGA LQGAGLGTNT SLAQMVSGLV GQLLMQPVLV 550
AQGTPGMAPA SASAPATAQA QAPAPAPAPA PAPATASASA GTTNTATTAG 600
PAPGGPAQPP PPQPSAADLQ FSQLLGNLLG PAGPGAGGPS LASPTITVAV 650
PGVPAFLQGM TEFLQASQAA PPPPPPPPPP PPAPEQQTTP PPGSPSGGTA 700
SPGGLGPESL PPEFFTSVVQ GVLSSLLGSL GARAGSSESI AAFIQRLSGS 750
SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR 800
SFFHQHYLGG QEPTSSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR 850
TNLEFLQEQF NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG 900
QQMELAAVIN GRIRRMSRGV NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI 950
LRYVRRIGDP PQALPEEPME VQGAERTSPE PQREDASPAP GTTAEEAMSR 1000
GPPPAPEGGS RDEQDGASAD AEPWAAAVPP EWVPIIQQDI QSQRKVKPQP 1050
PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS 1100
RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAHR AFADDP 1146
Length:1,146
Mass (Da):120,012
Last modified:January 11, 2011 - v2
Checksum:i9CAA4CD9C04F3C29
GO
Isoform 2 (identifier: Q6MG49-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     522-522: Missing.
     1067-1115: Missing.

Show »
Length:1,096
Mass (Da):114,647
Checksum:iE372D70E024D8F15
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei522 – 5221Missing in isoform 2.
VSP_040420
Alternative sequencei1067 – 111549Missing in isoform 2.
VSP_040421Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → N in BAA76607. 1 Publication
Sequence conflicti11 – 111M → V in BAA76607. 1 Publication
Sequence conflicti47 – 471A → R in BAA76607. 1 Publication
Sequence conflicti74 – 741D → E in BAA76607. 1 Publication
Sequence conflicti93 – 931Q → H in BAA76607. 1 Publication
Sequence conflicti114 – 1141L → C in BAA76607. 1 Publication
Sequence conflicti189 – 1891T → N in BAA76607. 1 Publication
Sequence conflicti264 – 2652NH → TQ in BAA76607. 1 Publication
Sequence conflicti567 – 5671T → TAQ in BAA76607. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018791 mRNA. Translation: BAA76607.1.
BX883045 Genomic DNA. Translation: CAE83997.1.
CH474121 Genomic DNA. Translation: EDL83534.1.
BC100141 mRNA. Translation: AAI00142.1.
RefSeqiNP_001029140.1. NM_001033968.1. [Q6MG49-1]
NP_446061.2. NM_053609.2. [Q6MG49-2]
UniGeneiRn.203343.

Genome annotation databases

EnsembliENSRNOT00000001129; ENSRNOP00000001129; ENSRNOG00000000851. [Q6MG49-2]
ENSRNOT00000060832; ENSRNOP00000057557; ENSRNOG00000000851. [Q6MG49-1]
GeneIDi94342.
KEGGirno:94342.
UCSCiRGD:71064. rat. [Q6MG49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018791 mRNA. Translation: BAA76607.1 .
BX883045 Genomic DNA. Translation: CAE83997.1 .
CH474121 Genomic DNA. Translation: EDL83534.1 .
BC100141 mRNA. Translation: AAI00142.1 .
RefSeqi NP_001029140.1. NM_001033968.1. [Q6MG49-1 ]
NP_446061.2. NM_053609.2. [Q6MG49-2 ]
UniGenei Rn.203343.

3D structure databases

ProteinModelPortali Q6MG49.
SMRi Q6MG49. Positions 17-101.
ModBasei Search...

Protein-protein interaction databases

BioGridi 250197. 1 interaction.
IntActi Q6MG49. 2 interactions.
MINTi MINT-4566953.
STRINGi 10116.ENSRNOP00000057557.

PTM databases

PhosphoSitei Q6MG49.

Proteomic databases

PaxDbi Q6MG49.
PRIDEi Q6MG49.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001129 ; ENSRNOP00000001129 ; ENSRNOG00000000851 . [Q6MG49-2 ]
ENSRNOT00000060832 ; ENSRNOP00000057557 ; ENSRNOG00000000851 . [Q6MG49-1 ]
GeneIDi 94342.
KEGGi rno:94342.
UCSCi RGD:71064. rat. [Q6MG49-1 ]

Organism-specific databases

CTDi 7917.
RGDi 71064. Bag6.

Phylogenomic databases

eggNOGi NOG297129.
GeneTreei ENSGT00390000016199.
HOGENOMi HOG000095177.
HOVERGENi HBG002193.
InParanoidi Q498N5.
OMAi QEPTPGN.
OrthoDBi EOG7B5WXH.
PhylomeDBi Q6MG49.
TreeFami TF328437.

Miscellaneous databases

NextBioi 617937.
PROi Q6MG49.

Gene expression databases

Genevestigatori Q6MG49.

Family and domain databases

InterProi IPR021925. DUF3538.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view ]
Pfami PF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
SMARTi SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiBAG6_RAT
AccessioniPrimary (citable) accession number: Q6MG49
Secondary accession number(s): Q498N5, Q9WTN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 11, 2011
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi