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Protein

ATP-binding cassette sub-family F member 1

Gene

Abcf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient Cap- and IRES-mediated mRNA translation initiation. Not involved in the ribosome biogenesis (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi330 – 3378ATP 1PROSITE-ProRule annotation
Nucleotide bindingi652 – 6598ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • positive regulation of translation Source: UniProtKB
  • translation Source: RGD
  • translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-382556. ABC-family proteins mediated transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family F member 1
Alternative name(s):
ATP-binding cassette 50
Gene namesi
Name:Abcf1
Synonyms:Abc50
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi620286. Abcf1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleusnucleoplasm By similarity
  • Nucleus envelope By similarity

GO - Cellular componenti

  • membrane Source: Ensembl
  • nuclear envelope Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • polysomal ribosome Source: UniProtKB
  • ribosome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 839839ATP-binding cassette sub-family F member 1PRO_0000093322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221PhosphoserineBy similarity
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei109 – 1091PhosphoserineCombined sources
Modified residuei140 – 1401PhosphoserineCombined sources
Modified residuei195 – 1951PhosphothreonineCombined sources
Modified residuei197 – 1971PhosphoserineCombined sources
Modified residuei227 – 2271PhosphoserineCombined sources
Cross-linki567 – 567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki576 – 576Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei589 – 5891PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at phosphoserine and phosphothreonine. Phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6MG08.
PRIDEiQ6MG08.

PTM databases

iPTMnetiQ6MG08.
PhosphoSiteiQ6MG08.

Expressioni

Gene expression databases

GenevisibleiQ6MG08. RN.

Interactioni

Subunit structurei

Interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Associates (via both ABC transporter domains) with the ribosomes (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001049.

Structurei

3D structure databases

ProteinModelPortaliQ6MG08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini298 – 542245ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini619 – 834216ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi85 – 939Poly-Asp
Compositional biasi139 – 14911Poly-GluAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0066. Eukaryota.
COG0488. LUCA.
GeneTreeiENSGT00630000089843.
HOGENOMiHOG000271637.
HOVERGENiHBG050440.
InParanoidiQ6MG08.
KOiK06184.
OMAiKKLTHKE.
OrthoDBiEOG7WDN23.
PhylomeDBiQ6MG08.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032781. ABC_tran_Xtn.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF12848. ABC_tran_Xtn. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MG08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKGPKQQPP EPEWIGDGEG TSPADKVVKK GKKDKKTKKT FFEELAVEDK
60 70 80 90 100
QAGEEEKLQK EKEQQQQQQQ QKKKRDTRKG RRKKDVDDDD DGDERVLMER
110 120 130 140 150
LKQLSVPASD EEDEVPVPVP RGRKKAKGGN VFEALIQDES EEEKEEEEEK
160 170 180 190 200
PVLKPAKPEK NRINKAVAEE PPGLRNKKGK EEKSKGKAKN KPSATDSEGE
210 220 230 240 250
DDEDMTKEKE PPRPGKDKDK KGAEQGSEEE KEEKEGEVKA NDPYAHLSKK
260 270 280 290 300
EKKKLKKQMD YERQVESLKA ANAAENDFSV SQAEVSSRQA MLENASDIKL
310 320 330 340 350
EKFSISAHGK ELFVNADLYI VAGRRYGLVG PNGKGKTTLL KHIANRALSI
360 370 380 390 400
PPNIDVLLCE QEVVADETPA VQAVLRADTK RLRLLEEEKR LQGQLEQGDD
410 420 430 440 450
TAAEKLEKVY EELRATGAAA AEAKARRILA GLGFDPEMQN RPTQKFSGGW
460 470 480 490 500
RMRVSLARAL FMEPTLLMLD EPTNHLDLNA VIWLNNYLQG WRKTLLIVSH
510 520 530 540 550
DQGFLDDVCT DIIHLDTQRL HYYRGNYMTF KKMYQQKQKE LLKQYEKQEK
560 570 580 590 600
KLKELKAGGK STKQAEKQTK EVLTRKQQKC RRKNQDEESQ DPPELLKRPR
610 620 630 640 650
EYTVRFTFPD PPPLSPPVLG LHGVTFGYEG QKPLFKNLDF GIDMDSRICI
660 670 680 690 700
VGPNGVGKST LLLLLTGKLT PTNGEMRKNH RLKIGFFNQQ YAEQLHMEET
710 720 730 740 750
PTEYLQRGFN LPYQDARKCL GRFGLESHAH TIQICKLSGG QKARVVFAEL
760 770 780 790 800
ACREPDVLIL DEPTNNLDIE SIDALGEAIN EYKGAVIVVS HDARLITETN
810 820 830
CQLWVVEEQS VSQIDGDFDD YKREVLEALG EVMVNRPRD
Length:839
Mass (Da):95,252
Last modified:July 5, 2004 - v1
Checksum:i0EB651DD0B3F5B24
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011D → DK in AAG23960 (PubMed:10931828).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883048 Genomic DNA. Translation: CAE84039.1.
AF293383 mRNA. Translation: AAG23960.1.
RefSeqiNP_001103353.1. NM_001109883.2.
UniGeneiRn.14350.

Genome annotation databases

EnsembliENSRNOT00000001049; ENSRNOP00000001049; ENSRNOG00000000799.
GeneIDi85493.
KEGGirno:85493.
UCSCiRGD:620286. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883048 Genomic DNA. Translation: CAE84039.1.
AF293383 mRNA. Translation: AAG23960.1.
RefSeqiNP_001103353.1. NM_001109883.2.
UniGeneiRn.14350.

3D structure databases

ProteinModelPortaliQ6MG08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001049.

PTM databases

iPTMnetiQ6MG08.
PhosphoSiteiQ6MG08.

Proteomic databases

PaxDbiQ6MG08.
PRIDEiQ6MG08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001049; ENSRNOP00000001049; ENSRNOG00000000799.
GeneIDi85493.
KEGGirno:85493.
UCSCiRGD:620286. rat.

Organism-specific databases

CTDi23.
RGDi620286. Abcf1.

Phylogenomic databases

eggNOGiKOG0066. Eukaryota.
COG0488. LUCA.
GeneTreeiENSGT00630000089843.
HOGENOMiHOG000271637.
HOVERGENiHBG050440.
InParanoidiQ6MG08.
KOiK06184.
OMAiKKLTHKE.
OrthoDBiEOG7WDN23.
PhylomeDBiQ6MG08.

Enzyme and pathway databases

ReactomeiR-RNO-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiQ6MG08.

Gene expression databases

GenevisibleiQ6MG08. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032781. ABC_tran_Xtn.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF12848. ABC_tran_Xtn. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "ABC50 interacts with eukaryotic initiation factor 2 and associates with the ribosome in an ATP-dependent manner."
    Tyzack J.K., Wang X., Belsham G.J., Proud C.G.
    J. Biol. Chem. 275:34131-34139(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-839, FUNCTION, ASSOCIATION WITH RIBOSOMES, INTERACTION WITH EIF2.
    Strain: Wistar.
    Tissue: Skeletal muscle.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109; SER-140; THR-195; SER-197 AND SER-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiABCF1_RAT
AccessioniPrimary (citable) accession number: Q6MG08
Secondary accession number(s): Q9ERQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.