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Q6MG08 (ABCF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family F member 1
Alternative name(s):
ATP-binding cassette 50
Gene names
Name:Abcf1
Synonyms:Abc50
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length839 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for efficient Cap- and IRES-mediated mRNA translation initiation. Not involved in the ribosome biogenesis By similarity. Ref.2

Subunit structure

Interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Associates (via both ABC transporter domains) with the ribosomes By similarity. Ref.2

Subcellular location

Cytoplasm By similarity. Nucleus › nucleoplasm By similarity. Nucleus envelope By similarity.

Post-translational modification

Phosphorylated at phosphoserine and phosphothreonine. Phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes By similarity.

Sequence similarities

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 839839ATP-binding cassette sub-family F member 1
PRO_0000093322

Regions

Domain298 – 542245ABC transporter 1
Domain619 – 834216ABC transporter 2
Nucleotide binding330 – 3378ATP 1 By similarity
Nucleotide binding652 – 6598ATP 2 By similarity
Compositional bias85 – 939Poly-Asp
Compositional bias139 – 14911Poly-Glu

Amino acid modifications

Modified residue221Phosphoserine By similarity
Modified residue1051Phosphoserine By similarity
Modified residue1091Phosphoserine; by CK2 By similarity
Modified residue1401Phosphoserine; by CK2 By similarity
Modified residue1971Phosphoserine By similarity
Modified residue5891Phosphoserine By similarity
Cross-link567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link576Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict2011D → DK in AAG23960. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q6MG08 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 0EB651DD0B3F5B24

FASTA83995,252
        10         20         30         40         50         60 
MPKGPKQQPP EPEWIGDGEG TSPADKVVKK GKKDKKTKKT FFEELAVEDK QAGEEEKLQK 

        70         80         90        100        110        120 
EKEQQQQQQQ QKKKRDTRKG RRKKDVDDDD DGDERVLMER LKQLSVPASD EEDEVPVPVP 

       130        140        150        160        170        180 
RGRKKAKGGN VFEALIQDES EEEKEEEEEK PVLKPAKPEK NRINKAVAEE PPGLRNKKGK 

       190        200        210        220        230        240 
EEKSKGKAKN KPSATDSEGE DDEDMTKEKE PPRPGKDKDK KGAEQGSEEE KEEKEGEVKA 

       250        260        270        280        290        300 
NDPYAHLSKK EKKKLKKQMD YERQVESLKA ANAAENDFSV SQAEVSSRQA MLENASDIKL 

       310        320        330        340        350        360 
EKFSISAHGK ELFVNADLYI VAGRRYGLVG PNGKGKTTLL KHIANRALSI PPNIDVLLCE 

       370        380        390        400        410        420 
QEVVADETPA VQAVLRADTK RLRLLEEEKR LQGQLEQGDD TAAEKLEKVY EELRATGAAA 

       430        440        450        460        470        480 
AEAKARRILA GLGFDPEMQN RPTQKFSGGW RMRVSLARAL FMEPTLLMLD EPTNHLDLNA 

       490        500        510        520        530        540 
VIWLNNYLQG WRKTLLIVSH DQGFLDDVCT DIIHLDTQRL HYYRGNYMTF KKMYQQKQKE 

       550        560        570        580        590        600 
LLKQYEKQEK KLKELKAGGK STKQAEKQTK EVLTRKQQKC RRKNQDEESQ DPPELLKRPR 

       610        620        630        640        650        660 
EYTVRFTFPD PPPLSPPVLG LHGVTFGYEG QKPLFKNLDF GIDMDSRICI VGPNGVGKST 

       670        680        690        700        710        720 
LLLLLTGKLT PTNGEMRKNH RLKIGFFNQQ YAEQLHMEET PTEYLQRGFN LPYQDARKCL 

       730        740        750        760        770        780 
GRFGLESHAH TIQICKLSGG QKARVVFAEL ACREPDVLIL DEPTNNLDIE SIDALGEAIN 

       790        800        810        820        830 
EYKGAVIVVS HDARLITETN CQLWVVEEQS VSQIDGDFDD YKREVLEALG EVMVNRPRD

« Hide

References

« Hide 'large scale' references
[1]"The genomic sequence and comparative analysis of the rat major histocompatibility complex."
Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"ABC50 interacts with eukaryotic initiation factor 2 and associates with the ribosome in an ATP-dependent manner."
Tyzack J.K., Wang X., Belsham G.J., Proud C.G.
J. Biol. Chem. 275:34131-34139(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-839, FUNCTION, ASSOCIATION WITH RIBOSOMES, INTERACTION WITH EIF2.
Strain: Wistar.
Tissue: Skeletal muscle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX883048 Genomic DNA. Translation: CAE84039.1.
AF293383 mRNA. Translation: AAG23960.1.
IPIIPI00421969.
RefSeqNP_001103353.1. NM_001109883.2.
UniGeneRn.14350.

3D structure databases

ProteinModelPortalQ6MG08.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000001049.

PTM databases

PhosphoSiteQ6MG08.

Proteomic databases

PaxDbQ6MG08.
PRIDEQ6MG08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000001049; ENSRNOP00000001049; ENSRNOG00000000799.
GeneID85493.
KEGGrno:85493.
UCSCRGD:620286. rat.

Organism-specific databases

CTD23.
RGD620286. Abcf1.

Phylogenomic databases

eggNOGCOG0488.
GeneTreeENSGT00630000089843.
HOGENOMHOG000271637.
HOVERGENHBG050440.
InParanoidQ6MG08.
KOK06184.
OrthoDBEOG4Z36DF.

Gene expression databases

ArrayExpressQ6MG08.
GenevestigatorQ6MG08.
GermOnlineENSRNOG00000000799. Rattus norvegicus.

Family and domain databases

InterProIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio617588.

Entry information

Entry nameABCF1_RAT
AccessionPrimary (citable) accession number: Q6MG08
Secondary accession number(s): Q9ERQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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