Q6MEK1 (RNC_PARUW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	pc0274

Organism

Protochlamydia amoebophila (strain UWE25) [Complete proteome] [HAMAP]

Taxonomic identifier

264201 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Parachlamydiaceae › Candidatus Protochlamydia › Candidatus Protochlamydia amoebophila

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity. HAMAP-Rule MF_00104
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00104
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000228559
Regions
	Domain	18 – 146	129	RNase III
	Domain	172 – 241	70	DRBM
Sites
	Active site	63	1	Potential
	Active site	135	1	By similarity
	Metal binding	59	1	Magnesium By similarity
	Metal binding	132	1	Magnesium By similarity
	Metal binding	135	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6MEK1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B03C64D8A37D8CC1	FASTA	242	27,542
	10 20 30 40 50 60 MLPRILYMNP IEHVIRQAPA IEAKLGYTFK DPQLLVLAFV HRSFINENRE VNQHNERLEF 70 80 90 100 110 120 LGDSVLGMLI SDYLYCKLPK TPEGQLSYLR SRLVEASSCV HYIQSLDLSG YLLLGKGERM 130 140 150 160 170 180 NDGRGRESIL ADLFEAIIGA IYLDGGLQAA KDFLFKNFHQ HIEIILATPL RNWKALLQDY 190 200 210 220 230 240 CQKNYQQTPL YQVLHESGPD HSKVFQISVW IQDRELGRGK GTSKKEAQQA AAADALSRVE LP

References

[1]

"Illuminating the evolutionary history of chlamydiae." Horn M., Collingro A., Schmitz-Esser S., Beier C.L., Purkhold U., Fartmann B., Brandt P., Nyakatura G.J., Droege M., Frishman D., Rattei T., Mewes H.-W., Wagner M. Science 304:728-730(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UWE25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX908798 Genomic DNA. Translation: CAF22998.1.
RefSeq	YP_007273.1. NC_005861.1.
3D structure databases
ProteinModelPortal	Q6MEK1.
ModBase	Search...
Protein-protein interaction databases
STRING	264201.pc0274.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAF22998; CAF22998; pc0274.
GeneID	2780184.
KEGG	pcu:pc0274.
PATRIC	31995842. VBICanPro72727_0281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
ProtClustDB	CLSK2762116.
Enzyme and pathway databases
BioCyc	PAMO264201:GH0M-278-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_PARUW

Accession

Primary (citable) accession number: Q6MEK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program