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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Protochlamydia amoebophila (strain UWE25)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPAMO264201:GH0M-506-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:pc0494
OrganismiProtochlamydia amoebophila (strain UWE25)
Taxonomic identifieri264201 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesParachlamydiaceaeCandidatus Protochlamydia
ProteomesiUP000000529 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Isoleucine--tRNA ligasePRO_0000098553Add
BLAST

Proteomic databases

PRIDEiQ6MDY1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264201.pc0494.

Structurei

3D structure databases

ProteinModelPortaliQ6MDY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiREGCEEF.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MDY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEEVLQESF DEREKKVLKF WQEGQLFERS VENRKGQPLF TFYDGPPFAT
60 70 80 90 100
GLPHYGHILA GTIKDVVLRY KTMKGFCAPR RFGWDCHGLP VENEIEKTFG
110 120 130 140 150
LSGAKSIEEF GIAKFNEECR NIVLRYTEEW KFTVNRMGRW VDFNQTYRTM
160 170 180 190 200
DLPFMESVWW VFKQLYAKGL VYEGLKVMPF SAKLGTPLSN FEASENYKEV
210 220 230 240 250
DDPSLTVAFQ SRDNSNTYFL AWTTTPWTLV SNLALMVSPM IEYAEVQDHV
260 270 280 290 300
SKRNYILATE RLKGYYKDSG EYTIVRKFPG SELEGQHYIP LFDYFNDRAH
310 320 330 340 350
SGAFKIILED SISVEEGTGI VQTAPAFGEI DFYACQKAGI DPVCPVDNNG
360 370 380 390 400
QFTDEIPEYK GIFVKEADKD IIKRLKQQAK VIHQGTCHHR YPFCPRSDTP
410 420 430 440 450
LIYKTVRTWF VAVEKIKDRL LAANSQIHWT PEHIQYGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPLWRAQDGE IHVVGSIEEL KQLTGNPLTD LHRHFIDEMS
510 520 530 540 550
FEKNGKTFKR IPEVFDCWFE SGSMPYAQNH YPFENRELFE QNFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVLSAALF DQPAMKNVIV NGLILAENGA KMSKRLKNYP
610 620 630 640 650
DPAEVIQQYG ADAIRLYMLH SPAVKADDLS FSKSGVELVL RQILLPLWNA
660 670 680 690 700
YTFFLTYARI YNWKPGKLVQ KPELAIDQWI ISLLNKLVHE VEQGMDDYDL
710 720 730 740 750
SRSVEPFVNF VDQLTNWYIR RSRRRFWDDK ESPNRTQAFE TLYYVLIELT
760 770 780 790 800
KISAPYVPFI SEAIYQNLRS CDMPESVHLC DFPHYQQLSR HEKLEAEMEA
810 820 830 840 850
VQVTVSLGHA LRKEHKLKVR QPLATAQLAS ADPKVLDFLK EQQHLISEEL
860 870 880 890 900
NVKEITFSSN EKDFVSLKAK PNFRVLGKKV GKLMKLAQLT IEQFGQKELT
910 920 930 940 950
ELLNHRSVEI ILEGHPVLLT SEDVQVERIV REGIIAANQG TITIALNTNL
960 970 980 990 1000
NKELLLEGLA REIVNKVNTM RREANFAVTD RIQLYMQTTT RVIECFDQYK
1010 1020 1030
NYICQEVLAT DVQFGPYEGT DWDLNGEPTK IIIKKSEY
Length:1,038
Mass (Da):120,225
Last modified:July 5, 2004 - v1
Checksum:i9A85E181CB977895
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX908798 Genomic DNA. Translation: CAF23218.1.
RefSeqiWP_011175044.1. NC_005861.1.

Genome annotation databases

EnsemblBacteriaiCAF23218; CAF23218; pc0494.
KEGGipcu:pc0494.
PATRICi31996292. VBICanPro72727_0498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX908798 Genomic DNA. Translation: CAF23218.1.
RefSeqiWP_011175044.1. NC_005861.1.

3D structure databases

ProteinModelPortaliQ6MDY1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264201.pc0494.

Proteomic databases

PRIDEiQ6MDY1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF23218; CAF23218; pc0494.
KEGGipcu:pc0494.
PATRICi31996292. VBICanPro72727_0498.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiREGCEEF.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciPAMO264201:GH0M-506-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UWE25.

Entry informationi

Entry nameiSYI_PARUW
AccessioniPrimary (citable) accession number: Q6MDY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.