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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Protochlamydia amoebophila (strain UWE25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPRO264201:G1G3R-481-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:pc0494
OrganismiProtochlamydia amoebophila (strain UWE25)
Taxonomic identifieri264201 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeParachlamydialesParachlamydiaceaeCandidatus Protochlamydia
Proteomesi
  • UP000000529 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985531 – 1038Isoleucine--tRNA ligaseAdd BLAST1038

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264201.pc0494

Structurei

3D structure databases

ProteinModelPortaliQ6MDY1
SMRiQ6MDY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q6MDY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEEVLQESF DEREKKVLKF WQEGQLFERS VENRKGQPLF TFYDGPPFAT
60 70 80 90 100
GLPHYGHILA GTIKDVVLRY KTMKGFCAPR RFGWDCHGLP VENEIEKTFG
110 120 130 140 150
LSGAKSIEEF GIAKFNEECR NIVLRYTEEW KFTVNRMGRW VDFNQTYRTM
160 170 180 190 200
DLPFMESVWW VFKQLYAKGL VYEGLKVMPF SAKLGTPLSN FEASENYKEV
210 220 230 240 250
DDPSLTVAFQ SRDNSNTYFL AWTTTPWTLV SNLALMVSPM IEYAEVQDHV
260 270 280 290 300
SKRNYILATE RLKGYYKDSG EYTIVRKFPG SELEGQHYIP LFDYFNDRAH
310 320 330 340 350
SGAFKIILED SISVEEGTGI VQTAPAFGEI DFYACQKAGI DPVCPVDNNG
360 370 380 390 400
QFTDEIPEYK GIFVKEADKD IIKRLKQQAK VIHQGTCHHR YPFCPRSDTP
410 420 430 440 450
LIYKTVRTWF VAVEKIKDRL LAANSQIHWT PEHIQYGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPLWRAQDGE IHVVGSIEEL KQLTGNPLTD LHRHFIDEMS
510 520 530 540 550
FEKNGKTFKR IPEVFDCWFE SGSMPYAQNH YPFENRELFE QNFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVLSAALF DQPAMKNVIV NGLILAENGA KMSKRLKNYP
610 620 630 640 650
DPAEVIQQYG ADAIRLYMLH SPAVKADDLS FSKSGVELVL RQILLPLWNA
660 670 680 690 700
YTFFLTYARI YNWKPGKLVQ KPELAIDQWI ISLLNKLVHE VEQGMDDYDL
710 720 730 740 750
SRSVEPFVNF VDQLTNWYIR RSRRRFWDDK ESPNRTQAFE TLYYVLIELT
760 770 780 790 800
KISAPYVPFI SEAIYQNLRS CDMPESVHLC DFPHYQQLSR HEKLEAEMEA
810 820 830 840 850
VQVTVSLGHA LRKEHKLKVR QPLATAQLAS ADPKVLDFLK EQQHLISEEL
860 870 880 890 900
NVKEITFSSN EKDFVSLKAK PNFRVLGKKV GKLMKLAQLT IEQFGQKELT
910 920 930 940 950
ELLNHRSVEI ILEGHPVLLT SEDVQVERIV REGIIAANQG TITIALNTNL
960 970 980 990 1000
NKELLLEGLA REIVNKVNTM RREANFAVTD RIQLYMQTTT RVIECFDQYK
1010 1020 1030
NYICQEVLAT DVQFGPYEGT DWDLNGEPTK IIIKKSEY
Length:1,038
Mass (Da):120,225
Last modified:July 5, 2004 - v1
Checksum:i9A85E181CB977895
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX908798 Genomic DNA Translation: CAF23218.1
RefSeqiWP_011175044.1, NC_005861.1

Genome annotation databases

EnsemblBacteriaiCAF23218; CAF23218; pc0494
KEGGipcu:pc0494

Similar proteinsi

Entry informationi

Entry nameiSYI_PARUW
AccessioniPrimary (citable) accession number: Q6MDY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health