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Q6MDY1

- SYI_PARUW

UniProt

Q6MDY1 - SYI_PARUW

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Protochlamydia amoebophila (strain UWE25)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPAMO264201:GH0M-506-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:pc0494
OrganismiProtochlamydia amoebophila (strain UWE25)
Taxonomic identifieri264201 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesParachlamydiaceaeCandidatus Protochlamydia
ProteomesiUP000000529: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Isoleucine--tRNA ligasePRO_0000098553Add
BLAST

Proteomic databases

PRIDEiQ6MDY1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264201.pc0494.

Structurei

3D structure databases

ProteinModelPortaliQ6MDY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6MDY1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFEEVLQESF DEREKKVLKF WQEGQLFERS VENRKGQPLF TFYDGPPFAT
60 70 80 90 100
GLPHYGHILA GTIKDVVLRY KTMKGFCAPR RFGWDCHGLP VENEIEKTFG
110 120 130 140 150
LSGAKSIEEF GIAKFNEECR NIVLRYTEEW KFTVNRMGRW VDFNQTYRTM
160 170 180 190 200
DLPFMESVWW VFKQLYAKGL VYEGLKVMPF SAKLGTPLSN FEASENYKEV
210 220 230 240 250
DDPSLTVAFQ SRDNSNTYFL AWTTTPWTLV SNLALMVSPM IEYAEVQDHV
260 270 280 290 300
SKRNYILATE RLKGYYKDSG EYTIVRKFPG SELEGQHYIP LFDYFNDRAH
310 320 330 340 350
SGAFKIILED SISVEEGTGI VQTAPAFGEI DFYACQKAGI DPVCPVDNNG
360 370 380 390 400
QFTDEIPEYK GIFVKEADKD IIKRLKQQAK VIHQGTCHHR YPFCPRSDTP
410 420 430 440 450
LIYKTVRTWF VAVEKIKDRL LAANSQIHWT PEHIQYGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPLWRAQDGE IHVVGSIEEL KQLTGNPLTD LHRHFIDEMS
510 520 530 540 550
FEKNGKTFKR IPEVFDCWFE SGSMPYAQNH YPFENRELFE QNFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVLSAALF DQPAMKNVIV NGLILAENGA KMSKRLKNYP
610 620 630 640 650
DPAEVIQQYG ADAIRLYMLH SPAVKADDLS FSKSGVELVL RQILLPLWNA
660 670 680 690 700
YTFFLTYARI YNWKPGKLVQ KPELAIDQWI ISLLNKLVHE VEQGMDDYDL
710 720 730 740 750
SRSVEPFVNF VDQLTNWYIR RSRRRFWDDK ESPNRTQAFE TLYYVLIELT
760 770 780 790 800
KISAPYVPFI SEAIYQNLRS CDMPESVHLC DFPHYQQLSR HEKLEAEMEA
810 820 830 840 850
VQVTVSLGHA LRKEHKLKVR QPLATAQLAS ADPKVLDFLK EQQHLISEEL
860 870 880 890 900
NVKEITFSSN EKDFVSLKAK PNFRVLGKKV GKLMKLAQLT IEQFGQKELT
910 920 930 940 950
ELLNHRSVEI ILEGHPVLLT SEDVQVERIV REGIIAANQG TITIALNTNL
960 970 980 990 1000
NKELLLEGLA REIVNKVNTM RREANFAVTD RIQLYMQTTT RVIECFDQYK
1010 1020 1030
NYICQEVLAT DVQFGPYEGT DWDLNGEPTK IIIKKSEY
Length:1,038
Mass (Da):120,225
Last modified:July 5, 2004 - v1
Checksum:i9A85E181CB977895
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX908798 Genomic DNA. Translation: CAF23218.1.
RefSeqiYP_007493.1. NC_005861.1.

Genome annotation databases

EnsemblBacteriaiCAF23218; CAF23218; pc0494.
GeneIDi2779943.
KEGGipcu:pc0494.
PATRICi31996292. VBICanPro72727_0498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX908798 Genomic DNA. Translation: CAF23218.1 .
RefSeqi YP_007493.1. NC_005861.1.

3D structure databases

ProteinModelPortali Q6MDY1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264201.pc0494.

Proteomic databases

PRIDEi Q6MDY1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAF23218 ; CAF23218 ; pc0494 .
GeneIDi 2779943.
KEGGi pcu:pc0494.
PATRICi 31996292. VBICanPro72727_0498.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PAMO264201:GH0M-506-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UWE25.

Entry informationi

Entry nameiSYI_PARUW
AccessioniPrimary (citable) accession number: Q6MDY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3