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Q6MBP0 (TRMB_PARUW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:pc1285
OrganismProtochlamydia amoebophila (strain UWE25) [Complete proteome] [HAMAP]
Taxonomic identifier264201 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesParachlamydiaceaeCandidatus Protochlamydia

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171367

Sites

Active site1311 By similarity
Binding site561S-adenosyl-L-methionine By similarity
Binding site811S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1311S-adenosyl-L-methionine By similarity
Binding site1351Substrate By similarity
Binding site1671Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6MBP0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: FD16CA5200615259

FASTA22526,668
        10         20         30         40         50         60 
MKPKDLKSPF KWEHRQIVIQ DRVWYVPDQH LSDIPFIFPG WTHDQTFAQD KPIYLEYCSG 

        70         80         90        100        110        120 
NGAWIASKAI EQSQYNWVGI EKKFDRTRKI WSKIKKFELD NLLTICGEGF HVTRDFFANE 

       130        140        150        160        170        180 
SVDAVFINFP DPWPKKRHAK HRIVQPAFVK EIHRILKSDK LFTLVTDDAA YSKIMIDVIY 

       190        200        210        220 
QTGGFISLFE APYYTTSYPG YGSSYFEELW REKGKSIHYH VFRKI 

« Hide

References

[1]"Illuminating the evolutionary history of chlamydiae."
Horn M., Collingro A., Schmitz-Esser S., Beier C.L., Purkhold U., Fartmann B., Brandt P., Nyakatura G.J., Droege M., Frishman D., Rattei T., Mewes H.-W., Wagner M.
Science 304:728-730(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UWE25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX908798 Genomic DNA. Translation: CAF24009.1.
RefSeqYP_008284.1. NC_005861.1.

3D structure databases

ProteinModelPortalQ6MBP0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264201.pc1285.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAF24009; CAF24009; pc1285.
GeneID2780395.
KEGGpcu:pc1285.
PATRIC31997960. VBICanPro72727_1314.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000034940.
KOK03439.
OMAIVELAMN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycPAMO264201:GH0M-1315-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PARUW
AccessionPrimary (citable) accession number: Q6MBP0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways