Q6MBP0 (TRMB_PARUW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	pc1285

Organism

Protochlamydia amoebophila (strain UWE25) [Complete proteome] [HAMAP]

Taxonomic identifier

264201 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Parachlamydiaceae › Candidatus Protochlamydia › Candidatus Protochlamydia amoebophila

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171367
Sites
	Binding site	56	1	S-adenosyl-L-methionine By similarity
	Binding site	81	1	S-adenosyl-L-methionine By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	131	1	S-adenosyl-L-methionine By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	167	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6MBP0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FD16CA5200615259	FASTA	225	26,668
	10 20 30 40 50 60 MKPKDLKSPF KWEHRQIVIQ DRVWYVPDQH LSDIPFIFPG WTHDQTFAQD KPIYLEYCSG 70 80 90 100 110 120 NGAWIASKAI EQSQYNWVGI EKKFDRTRKI WSKIKKFELD NLLTICGEGF HVTRDFFANE 130 140 150 160 170 180 SVDAVFINFP DPWPKKRHAK HRIVQPAFVK EIHRILKSDK LFTLVTDDAA YSKIMIDVIY 190 200 210 220 QTGGFISLFE APYYTTSYPG YGSSYFEELW REKGKSIHYH VFRKI

References

[1]

"Illuminating the evolutionary history of chlamydiae." Horn M., Collingro A., Schmitz-Esser S., Beier C.L., Purkhold U., Fartmann B., Brandt P., Nyakatura G.J., Droege M., Frishman D., Rattei T., Mewes H.-W., Wagner M. Science 304:728-730(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UWE25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX908798 Genomic DNA. Translation: CAF24009.1.
RefSeq	YP_008284.1. NC_005861.1.
3D structure databases
ProteinModelPortal	Q6MBP0.
ModBase	Search...
Protein-protein interaction databases
STRING	264201.pc1285.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAF24009; CAF24009; pc1285.
GeneID	2780395.
KEGG	pcu:pc1285.
PATRIC	31997960. VBICanPro72727_1314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000034940.
KO	K03439.
OMA	NLRIVCG.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	PAMO264201:GH0M-1286-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_PARUW

Accession

Primary (citable) accession number: Q6MBP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program