Q6M7C6 (DNAE2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Cgl0638, cg0738

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	1055 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.
Sequence caution	The sequence BAB98031.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1055	1055	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103378

Sequences

	Sequence		Length	Mass (Da)
	Q6M7C6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 91B4912894FEB6C2	FASTA	1,055	115,099
	10 20 30 40 50 60 MYPIIMEWNG GGSFNGRPLS WSKLERILSG KKVESLRPVL HEPDAQAPSS AMQGEASVPF 70 80 90 100 110 120 AELHATSSYN FLTGASDPSD VVVQAKKLGL VALSVMDRDG FYGAVRFAEA AAEAGMHTVY 130 140 150 160 170 180 GAELSLQEGV LTVLCKNPEG YKKLSHLISD AKMATGEKGE VRYPPLPMVA EHAAGDWVVL 190 200 210 220 230 240 AGFQWLDKID YVIDCFKPEN IVLEFGSTMT PEDADRNEYL RRTQAKFQLR GILSTNPESA 250 260 270 280 290 300 ARGSVRLAGA KQALARKMPL ADAESELHPM GTTWMRSGDT LLKAHPDYAD LIATTVELAA 310 320 330 340 350 360 ECAFTLDLVA PNLPKWDTPG EHTEMSWLAH LVSTRIDTRY VGRSADIKAR AATQIDYELG 370 380 390 400 410 420 VIEKLGFPGY FLVVNDLVEF CRDSNILCQG RGSAANSAVC FVLGITNAEP ISAGLLFERF 430 440 450 460 470 480 LSPDRDGPPD IDIDIESGRR EEVIQYVYEK YGRDNAAQVA NVITYRTKGA MRDAARALGY 490 500 510 520 530 540 PQGAADAWAK GTSEPPDDVL ELAAQFKGQP RHLGIHSGGM VICDRPIADV VPVEWARMDN 550 560 570 580 590 600 RSVVQWDKDD CATAGLVKFD LLGLGMLEAI HHMLDLVAEH RGKKINLWEL DLAEPEVYDM 610 620 630 640 650 660 LCKADAVGVF QVESRAQLST LPRLKPRTFF DLVVEVALIR PGPIQGGSVH PYLRRRAGEE 670 680 690 700 710 720 AITYDHPVLE KSLGKTLGIP LFQEQLMQVA VDAAGFSGGE ADSLRRAMGS KRSPERMAAL 730 740 750 760 770 780 RSRFFQGLKD TNGIVGETAE KLWNKIVAFA AYGFPESHSQ SFASLVYFSA WFKYHYPAEF 790 800 810 820 830 840 CVGLLRAQPM GFYSPQSLIS DARRHGVSIL PITVNDSGVE ADAPNGAIRL GLNLVKGLGH 850 860 870 880 890 900 DAAQRIEDNA PFDSIPDLSR RADLNVAQVE ALARAGAVDC LGVGRRQALW QAGVAATEKP 910 920 930 940 950 960 GMLPGLSVIE APALPGMSAF ELMATNISAT GVTADYQPMA LIRERMEELG IVPADRLLEV 970 980 990 1000 1010 1020 EDGTRLRIAG IVTHRQRPQT ASGLTFLGME DETGLMNVMV SVGLWQRQRV LARNAKALII 1030 1040 1050 RGIVQNAQGV ATVVADRLEP LDMGEFLSRG SRDFR

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98031.1. Different initiation. BX927149 Genomic DNA. Translation: CAF19345.1.
RefSeq	NP_599872.1. NC_003450.3. YP_224931.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q6M7C6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1018640. 3342922.
GenomeReviews	Gene locus Cgl0638 in contig BA000036_GR. Gene locus cg0738 in contig BX927147_GR.
KEGG	cgb:cg0738. cgl:NCgl0611.
PATRIC	21493316. VBICorGlu203724_0624.
Phylogenomic databases
HOGENOM	HBG734490.
OMA	YRAKGAI.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	CGLU196627:CG0738-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAE2_CORGL

Accession

Primary (citable) accession number: Q6M7C6
Secondary accession number(s): Q8NSN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program