Reviewed, UniProtKB/Swiss-Prot Q6M191 (DP2L_METMP)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MMP0026

Organism

Methanococcus maripaludis [Complete proteome] [HAMAP]

Taxonomic identifier

39152 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294686

Sequences

	Sequence		Length	Mass (Da)
	Q6M191-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3A000B7DAC5961DE	FASTA	1,131	128,381
	10 20 30 40 50 60 MLHVSASKGM TEYFKNILDD VSNLYNLAEE CRKNGYDVTD HVEIPLAKDM ADRVEGIVGP 70 80 90 100 110 120 KNVAERIREL VSEFGKEPAA LEIAKEIVEG KFGEFGREVG AEQAVRTALA VITEGIVAAP 130 140 150 160 170 180 LEGIAYVKIK KNSDNSEYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMG LDRFKPTEDE 190 200 210 220 230 240 VERYGEEVDL YQSEVTTFQY QPKAEEIRVA VRNISVEITG EATDDVEVSG HRDLPRVETN 250 260 270 280 290 300 QIRGGALLAL VEGVLLKAPK ILRHVDKLNI EGWNWLKELK SKKEEVIEEL EEENDEYNYE 310 320 330 340 350 360 DEEDLSQYED YEVEAVTKFI GEVIAGRPVF SHPSKKGGFR LRYGRSRNTG FATDGFHPAI 370 380 390 400 410 420 MYLVDDFMAV GTQLKTERPG KATCVVPVDS IEGPIVKLND GSVLKIDTVE KAKQYTDEVQ 430 440 450 460 470 480 EILFLGDILV NYGDFLENNH TVLPSSWCTE WYEKILKSQN LEYTEEFIKN PGQKEAVNYA 490 500 510 520 530 540 KITKTPLHPK YTYFWHDISK ENISTLRSWV IGGKYNPSND SWELNYDPED EEISNAKRYL 550 560 570 580 590 600 ELIGCPHIVM EEKVEIFEYY PFLYSLGYDF DEKRDMIDNI DEKLQNTKNN MHFINTIAPF 610 620 630 640 650 660 EIRRNAYIYV GARMGRPEKA AARKMKPPVN GLFPIGNAGA LVRLINKAVD EGKTDEIEIA 670 680 690 700 710 720 NVKCSCGKVS LYRTCPFCGN SVEPTGPSRI KLPIKDYWYK TLENLKINKP GDVKCIKGMT 730 740 750 760 770 780 SKDKIIEPLE KAILRAKNNI YVFKDGTTRF DCTDVPVTHF KPVEIHVPIE KLKSLGYLKD 790 800 810 820 830 840 IHGNPLENED QVLELKVQDV IVPESCMDYF LNVSGFIDDL LEKYYKKDRF YNVNTREDLV 850 860 870 880 890 900 GHLIIGMAPH TSAGMVGRII GYSNANVGYA HPYFHASKRR NCDGDEDAFF LLLDAFMNFS 910 920 930 940 950 960 KRFLPDKRGG QMDAPLVLTT ILDPKEVDGE VHNMDSMWEY PLEFYEKSLE GIAPKEIKKM 970 980 990 1000 1010 1020 METIEDRLDK DSQYEGIGYT HETSKIDEGP PICAYKTLGS MMEKTSAQLA VAKKIRATDE 1030 1040 1050 1060 1070 1080 RDVAEKVIQS HFVPDLIGNL RAFSRQGVRC KCGAKYRRMP LKGVCRKCGS RLILTVSKGA 1090 1100 1110 1120 1130 VEKYMNVSQT MAEKYDASDY IKQRLEIIKS GIDSLFVNDK RKQVKIEDFF K

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950229 Genomic DNA. Translation: CAF29582.1.
RefSeq	NP_987146.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2762226.
GenomeReviews	Gene locus MMP0026 in contig BX950229_GR.
KEGG	mmp:MMP0026.
NMPDR	fig|267377.1.peg.26.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6M191.
OMA	Q6M191. NCDGDED.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0026-MON.
BRENDA	2.7.7.7. 20956.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METMP

Accession

Primary (citable) accession number: Q6M191

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 5, 2004
Last modified:	May 5, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)