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Protein

Hypoxanthine/guanine phosphoribosyltransferase

Gene

hpt

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathway:iIMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine/guanine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-166-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRTase
Gene namesi
Name:hpt
Ordered Locus Names:MMP0145
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000590 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Can be used as a counterselectable marker when transforming this organism.1 Publication

Disruption phenotypei

Increased resistance to the base analog 8-azahypoxanthine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Hypoxanthine/guanine phosphoribosyltransferasePRO_0000149498Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi267377.MMP0145.

Structurei

3D structure databases

ProteinModelPortaliQ6M0X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0503.
HOGENOMiHOG000228520.
KOiK00759.
OMAiIATDIPY.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6M0X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLLEESLK TCPIVKRGEY HYFIHPISDG VPLVEPELLR DVSTRVIKMI
60 70 80 90 100
DTDVDKIVTA EAMGIPIVTA VSIATDIPYV IMRKREYLLE GEIPVHQETG
110 120 130 140 150
YSKGELYLNG INKGDKVIIL DDVISTGGTL VAIINALKRA GADIKDVLCI
160 170 180
IDRGNGQNIV EEKTGYKVKT IVKIEVVDGK VNILE
Length:185
Mass (Da):20,402
Last modified:July 5, 2004 - v1
Checksum:i4E99371CB6FE0EC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF29701.1.
RefSeqiNP_987265.1. NC_005791.1.
WP_011170089.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF29701; CAF29701; MMP0145.
GeneIDi2762740.
KEGGimmp:MMP0145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF29701.1.
RefSeqiNP_987265.1. NC_005791.1.
WP_011170089.1. NC_005791.1.

3D structure databases

ProteinModelPortaliQ6M0X3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267377.MMP0145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF29701; CAF29701; MMP0145.
GeneIDi2762740.
KEGGimmp:MMP0145.

Phylogenomic databases

eggNOGiCOG0503.
HOGENOMiHOG000228520.
KOiK00759.
OMAiIATDIPY.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciMMAR267377:GJ77-166-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S2 / LL.
  2. "Markerless mutagenesis in Methanococcus maripaludis demonstrates roles for alanine dehydrogenase, alanine racemase, and alanine permease."
    Moore B.C., Leigh J.A.
    J. Bacteriol. 187:972-979(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY, DISRUPTION PHENOTYPE.
    Strain: S2 / LL.

Entry informationi

Entry nameiHPRT_METMP
AccessioniPrimary (citable) accession number: Q6M0X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: May 27, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.