Q6M0T4 (RIFK_METMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	MMP0184

Organism

Methanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]

Taxonomic identifier

267377 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Riboflavin kinase HAMAP MF_01285		PRO_0000322071
Regions
	Nucleotide binding	10 – 15	6	CDP By similarity
	Nucleotide binding	109 – 112	4	CDP By similarity
Sites
	Metal binding	39	1	Magnesium By similarity
	Metal binding	41	1	Magnesium By similarity
	Binding site	96	1	FMN By similarity
	Binding site	104	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6M0T4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0CACEEADA69E0E43	FASTA	127	14,345
	10 20 30 40 50 60 MEIFGHVVSG LGEGKFFVGL THYKNKFEEL TGFTPFEGTL NVKLKHNFNL DEFNPIEFDG 70 80 90 100 110 120 FEIDGKKYFG GKVLLIKLFN KQGNSINCAV VAPKKTDHSK KTLEIIAPIQ LRKFLLLKNS DVVKLVI

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950229 Genomic DNA. Translation: CAF29740.1.
RefSeq	NP_987304.1. NC_005791.1.
3D structure databases
ProteinModelPortal	Q6M0T4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2761170.
GenomeReviews	Gene locus MMP0184 in contig BX950229_GR.
KEGG	mmp:MMP0184.
NMPDR	fig|267377.1.peg.184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	PYEGTLN.
ProtClustDB	PRK14132.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0184-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METMP

Accession

Primary (citable) accession number: Q6M0T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program