Q6M0P4 (G1PDH_METMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	MMP0225

Organism

Methanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]

Taxonomic identifier

267377 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000350657
Regions
	Nucleotide binding	77 – 81	5	NAD By similarity
	Nucleotide binding	99 – 102	4	NAD By similarity
Sites
	Metal binding	147	1	Zinc; catalytic By similarity
	Metal binding	225	1	Zinc; catalytic By similarity
	Metal binding	246	1	Zinc; catalytic By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6M0P4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1E7C2EA43D4D4047	FASTA	334	36,819
	10 20 30 40 50 60 MIVIPRYTII KEKASFRIQE ILDNLNLKNP LVITGKNTQK YNKDFDFIYY DEIETSDLEN 70 80 90 100 110 120 IKNYANDYDS VIGIGGGRPI DIGKLIAHKS KKPFLSVPTT ASNDGIASPI VSLTQPSYMT 130 140 150 160 170 180 EAPIAIIADI DIIKKSPKKL LSAGMGDIVS NITAVLDWEL GKIEKSEKYS DSSGIFSKTI 190 200 210 220 230 240 AIELMDYVLN SNLEEYPKKL VKALIGSGIS IAIAHSSRPA SGSEHLFSHA LDTMKEKYSI 250 260 270 280 290 300 DTNSLHGEQC GVGTLATAQI YLEEGKLEVE TFEMLKTSLK VVDAPVTAKQ LGFDEEIVIE 310 320 330 ALSSAHALRN RHTILRNGIS KEKAREILEK SEII

References

[1]

"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950229 Genomic DNA. Translation: CAF29781.1.
RefSeq	NP_987345.1. NC_005791.1.
3D structure databases
ProteinModelPortal	Q6M0P4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2761192.
GenomeReviews	Gene locus MMP0225 in contig BX950229_GR.
KEGG	mmp:MMP0225.
NMPDR	fig|267377.1.peg.225.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672951.
OMA	DKPALHG.
ProtClustDB	CLSK876301.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP0225-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METMP

Accession

Primary (citable) accession number: Q6M0P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program